LOCUS       sequence-331886-        9413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GPRC6A-Tango
FEATURES             Location/Qualifiers
     source          1..9413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3737..4351,4352..4738)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3804..3823)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4770..4787)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4776..5000
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5046..5474
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5133..5152)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5343..5364
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5483..5503)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5488..5817
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5668..5803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5730..5749
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5884..6678
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5938..5957)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6548..6567
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6852..6973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6889..6908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6943..6962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7022..7038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7022..7038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7035..7057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7046..7062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7070..7076,7077..7094,7095..7100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7115..7136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7253..7270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7424..8012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7504..7523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8183..8974,8975..9043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8806..8825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9044..9148)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9223..9242)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg cgccaccatg aagacgatca tcgccctgag
      781 ctacatcttc tgcctggtat tcgccgacta caaggacgat gatgacgcca gcatcgatat
      841 ggcatttctg ataatcctga ttacttgctt tgtgattatt ctggccacat cccaaccgtg
      901 tcaaaccccc gatgattttg tggcagcgac aagccccgga cacatcatta tcggtggcct
      961 gtttgctatt cacgaaaaaa tgctgagtag cgaggactct ccaagaagac cacagatcca
     1021 agaatgcgtg ggcttcgaga tcagtgtatt tctgcagacc ctggcaatga ttcatagtat
     1081 tgaaatgatc aataattcaa ccctgttgcc cggggtcaag ctggggtacg agatctatga
     1141 tacctgcaca gaagtcacag tggctatggc cgctacactt cgctttttgt ccaagttcaa
     1201 ttgttctcgc gagacagttg aattcaaatg cgactactcc tcctacatgc ccagggttaa
     1261 agccgtgatc ggaagcggct actctgaaat tacaatggct gtgtcccgga tgttgaactt
     1321 gcagctcatg ccccaagtag gatacgagag cacagcagaa atcctcagtg acaaaatccg
     1381 gttcccatcc ttcttgagga cggttcctag tgacttccac cagatcaagg ctatggctca
     1441 cctgattcaa aagagcggtt ggaattggat tggtattatt accacagatg atgactatgg
     1501 ccgactggca ttgaacacat ttatcatcca ggctgaagct aataacgtgt gtatcgcttt
     1561 taaagaagtg ttgccagcct ttctgagtga caacaccatc gaggtcagaa tcaaccgaac
     1621 actgaagaaa ataattctgg aagcgcaggt taacgtgatc gtagtctttc ttaggcagtt
     1681 ccacgttttc gacctcttca ataaggcaat tgaaatgaat attaataaga tgtggattgc
     1741 gtctgataac tggtctacag ccactaaaat caccactata cctaacgtta agaagattgg
     1801 aaaggtggtc ggttttgcat tcagacgagg caacatttcc agcttccact catttttgca
     1861 gaatctgcat ctgctcccat ccgacagcca caagctgttg cacgagtatg ccatgcactt
     1921 gagcgcttgc gcctacgtga aggacactga tctcagtcaa tgcatcttca accacagcca
     1981 acggaccctg gcttacaaag ccaacaaagc tatcgagagg aattttgtaa tgaggaatga
     2041 ttttctgtgg gactacgccg agcctggctt gattcattcc attcagctgg cagttttcgc
     2101 cctcggctac gcaattcgcg acctgtgcca ggccagagac tgccaaaatc ccaatgcttt
     2161 tcagccttgg gagctgctgg gggtgctgaa gaacgtaacc ttcaccgatg ggtggaattc
     2221 atttcatttt gacgcacacg gtgatctcaa tactgggtat gacgttgtcc tgtggaaaga
     2281 aatcaacggg cacatgacag tgacaaaaat ggctgagtac gacttgcaaa acgacgtctt
     2341 catcatcccc gatcaggaaa ccaaaaatga gttccgcaac ctgaagcaaa tacaaagtaa
     2401 atgctctaaa gaatgctctc ctggccagat gaaaaagaca acccggtccc agcacatatg
     2461 ctgttacgag tgtcaaaact gcccagagaa ccattataca aaccagacag atatgccaca
     2521 ctgtctgttg tgcaacaata aaacccattg ggctcctgtg cggtccacta tgtgcttcga
     2581 gaaggaggtg gagtatctga attggaacga ttctctggcc atcctcctgc ttatcctgtc
     2641 tcttctgggc attattttcg tcctcgttgt ggggattata ttcaccagga atcttaatac
     2701 acccgtcgtg aagtctagtg ggggtctgag ggtttgttat gtgattcttc tttgccactt
     2761 cttgaacttt gctagcacat ctttctttat cggagagccc caggatttca cttgcaagac
     2821 gagacagact atgtttggcg tctcctttac cctgtgtata tcttgtatcc tgacaaagag
     2881 cctcaagatc ctcctggcat tttcatttga ccctaagttg cagaagttct tgaaatgtct
     2941 ctatcgccca atcctgatca tattcacctg tacaggcatc caggttgtta tttgcaccct
     3001 gtggttgatc ttcgccgcac ctaccgttga ggtgaacgtt tctctgccgc gagttatcat
     3061 cttggagtgc gaggagggta gcatccttgc atttggtaca atgttgggct atatcgccat
     3121 cctggccttt atctgtttca tcttcgcatt taaggggaag tacgagaatt ataacgaggc
     3181 caagttcata acttttggta tgctgattta ctttatagca tggatcacct tcattccaat
     3241 ctatgccacc accttcggga aatacgttcc tgcagtagag attattgtga tccttatttc
     3301 taattacggc atcctgtact gcaccttcat tccgaaatgc tatgtaatta tttgcaaaca
     3361 agagataaac actaaatccg cctttctgaa gatgatttac agctattcct ctcatagtgt
     3421 gagtagtatc gctctgtcac cagccagtct ggacagtatg tccggtaacg ttactatgac
     3481 aaaccccagt tcttcaggca agtccgcaac ttggcagaaa tccaaggacc tgcaggccca
     3541 ggcctttgcc cacatctgcc gggagaacgc tacatcagtg agtaaaacac tcccgaggaa
     3601 gaggatgagc tccattatcg ataccggtgg acgcacccca cccagcctgg gtccccaaga
     3661 tgagtcctgc accaccgcca gctcctccct ggccaaggac acttcatcga ccggtgagaa
     3721 cctgtacttc cagctaagat tagataaaag taaagtgatt aacagcgcat tagagctgct
     3781 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
     3841 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
     3901 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
     3961 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
     4021 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
     4081 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
     4141 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
     4201 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
     4261 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
     4321 agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc cgcgcgcgta cgaaaaacaa
     4381 ttacgggtct accatcgagg gcctgctcga tctcccggac gacgacgccc ccgaagaggc
     4441 ggggctggcg gctccgcgcc tgtcctttct ccccgcggga cacacgcgca gactgtcgac
     4501 ggcccccccg accgatgtca gcctggggga cgagctccac ttagacggcg aggacgtggc
     4561 gatggcgcat gccgacgcgc tagacgattt cgatctggac atgttggggg acggggattc
     4621 cccgggtccg ggatttaccc cccacgactc cgccccctac ggcgctctgg atatggccga
     4681 cttcgagttt gagcagatgt ttaccgatgc ccttggaatt gacgagtacg gtgggtagtc
     4741 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4801 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4861 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4921 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4981 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     5041 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5101 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5161 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5221 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5281 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5341 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5401 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5461 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5521 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5581 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5641 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5701 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     5761 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5821 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     5881 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     5941 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6001 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6061 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6121 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6181 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6241 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     6301 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     6361 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     6421 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     6481 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     6541 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6601 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6661 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     6721 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6781 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6841 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6901 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6961 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     7021 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7081 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7141 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7201 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7261 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7321 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7381 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7441 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7501 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7561 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7621 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7681 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7741 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7801 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7861 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7921 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7981 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8041 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8101 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8161 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8221 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8281 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8341 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8401 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8461 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8521 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8581 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8641 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8701 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8761 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8821 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8881 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8941 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9001 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9061 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9121 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     9181 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     9241 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     9301 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     9361 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtt
//
