LOCUS       sequence-33189-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Shigetomi E, Kracun S, Khakh BS.
  TITLE     Monitoring astrocyte calcium microdomains with improved membrane 
            targeted GCaMP reporters.
  JOURNAL   Neuron Glia Biol. 2010 Dec 16:1-9. [Epub ahead of print]
  PUBMED    21205365
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron Glia
            Biol. 2010 Dec 16:1-9. [Epub ahead of print]"
COMMENT     Sequence Label: pN1-Lck-GCaMP3
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(79..100)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(313..369)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      381..390
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(397..411,412..420))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(424..456)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(460..477)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      603..612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(659..862)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(688..708)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcgcccttg ctcaccatgc tccctccggt accgcccttg tacagctcgt ccatgccgag
       61 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      121 gtctttcgaa agtttggact ggacgctcag gtagtggttg tcgggcagca gcacggggcc
      181 gtcgccgatg ggggtgttct gctggtagtg gtaggcgagc tgcacgccgc cgtcctcgat
      241 gttgtggcgg atcttgaagt tcgccttgat gccgttcttc tgcttgtcgg ccttgatata
      301 gacgttctcg agtgagctca gccgacctat agctctgact gcgtgacctg tcttattcca
      361 cttacgacgt gatgagtcga ccatggtggc gagatcctta tcgtcatcgt cgtacagatc
      421 ccgacccatt tgctgtccac cagtcatgct agccatacca tgatgatgat gatgatgaga
      481 accccgcatg gtggcgagat ccggtggatc aattcgaagc ttcggcgggg gataatggca
      541 gttctcacac acgtcaatgt tctccatcca gtcatcttca gggtttgagc tgcagccaca
      601 gcccatggtg gcagatctga gtccggtagc gctagcggat ctgacggttc actaaaccag
      661 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      721 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      781 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      841 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      901 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccagg
//
