LOCUS       sequence-331893-        2119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Frangedakis E, Guzman-Chavez F, Rebmann M, Markel K, Yu Y, Perraki 
            A, Tse SW, Liu Y, Rever J, Sauret-Gueto S, Goffinet B, Schneider H, 
            Haseloff J
  TITLE     Construction of DNA Tools for Hyperexpression in Marchantia 
            Chloroplasts.
  JOURNAL   ACS Synth Biol. 2021 Jul 16;10(7):1651-1666. doi: 
            10.1021/acssynbio.0c00637. Epub 2021 Jun 7.
  PUBMED    34097383
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00637"; journalName: "ACS Synth Biol"; date: 
            "2021-07-16- 16"; volume: "10"; issue: "7"; pages: "1651-1666"
COMMENT     Sequence Label: 5UTR_Mppetb_short_chlo
FEATURES             Location/Qualifiers
     source          1..2119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(455..549)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(570..1229)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1144..1163
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1391..1409
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1413..1456)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1551..1608
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1632..1649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1803..2119,1..272))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1883..1902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
       61 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      121 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
      361 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
      421 atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc
      481 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
      541 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
      601 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
      661 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
      721 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
      781 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
      841 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
      901 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
      961 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     1021 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     1081 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     1141 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     1201 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     1261 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     1321 acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt
     1381 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
     1441 cgctaaggat gatttctgga attcggtctc tcattattca aaaatccttg aatttaataa
     1501 ttacacaatt aaactaccta aaataaaaaa atgagtaaga gaccctgcag tccggcaaaa
     1561 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
     1621 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1681 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1741 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1801 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1861 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1921 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1981 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2041 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2101 aactatcgtc ttgagtcca
//
