LOCUS       sequence-331918-        3077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acceptor plasmid for odd level position 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: pCk1chlo
FEATURES             Location/Qualifiers
     source          1..3077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     544..563
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1347..1369
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1361..1378
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1362..1378
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1379..1435
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1448..1464)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1448..1464)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1461..1483)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1472..1488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1496..1502,1503..1520,1521..1526))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1541..1562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1788..1810)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1896..2036
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2051..2068)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2222..2810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2302..2321)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2898..3077,1..636))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(2898..3077,1..636))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
       61 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
      121 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
      181 cgcctgagcg agtcgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
      241 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
      301 ttcttctaat acctggaatg ctgttttccc tgggatcgca gtggtgagta accatgcatc
      361 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
      421 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
      481 caactctggc gcatcgggct tcccatacaa tcggtagatt gtcgcacctg attgcccgac
      541 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
      601 ccttgagcaa gacgtttccc gttgaatatg gctcataaca gaacttatta tttccttcct
      661 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
      721 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
      781 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
      841 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
      901 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
      961 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgaattcg cggccgcgct
     1021 cttcaatgag gagagagacc cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     1081 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     1141 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     1201 agaaaatacc gcatcaggcg ctcatccgcc attcaggctg cgcaactgtt gggaagggcg
     1261 atcggcgcag gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     1321 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     1381 attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt
     1441 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1501 tacgagccgg aagcatatag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1561 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctggggt
     1621 ctctcgctag catgaagagc gcggccgcct gcagtggccg ctcaaaaatg gctggcctac
     1681 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     1741 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     1801 cagctcccgg tgacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     1861 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     1921 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     1981 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     2041 catccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2101 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2161 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2221 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2281 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2341 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2401 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2461 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2521 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2581 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aattggtggc
     2641 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2701 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2761 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2821 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2881 tcatgcattc taggtgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     2941 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     3001 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     3061 ccaacatcaa tacaacc
//
