LOCUS       sequence-33192-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Elowitz MB, Leibler S
  TITLE     A synthetic oscillatory network of transcriptional regulators.
  JOURNAL   Nature. 2000 Jan 20. 403(6767):335-8.
  PUBMED    10659856
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2000 Jan 20. 403(6767):335-8."
COMMENT     Sequence Label: repressilator (pZS1-lTlrLLtCL)
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             53..64
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    106..122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(168..186)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        254..358
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(577..596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gctctaatgc gctgttaatc actttacttt tatctaatct ggacatggta cctttctcct
       61 ctttaatgaa ttcggtcagt gcgtcctgct gatgtgctca gtatcttgtt atccgctcac
      121 aatgtcaatt gttatccgct cacaattctc gaggtgaaga cgaaagggcc tcgtgatacg
      181 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      241 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      301 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      361 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      421 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      481 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      541 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      601 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      661 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      721 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      781 a
//
