LOCUS       sequence-331932-        6467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mEos3.2_H-NSdbd in Mycobacterium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Rashid FM, Mahlandt E, van der Vaart M, Boer DEC, Varela Alvarez M, 
            Henneman B, Brocken DJW, Voskamp P, Blok AJ, Shimizu TS, Meijer AH, 
            Luijsterburg MS, Goedhart J, Cremazy FGE, Dame RT
  TITLE     HI-NESS: a family of genetically encoded DNA labels based on a 
            bacterial nucleoid-associated protein.
  JOURNAL   Nucleic Acids Res. 2021 Nov 4. pii: 6414584. doi: 
            10.1093/nar/gkab993.
  PUBMED    34734265
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Nov 4. pii: 6414584. doi: 10.1093/nar/gkab993."
COMMENT     Sequence Label: pRD393
FEATURES             Location/Qualifiers
     source          1..6467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..57)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(42..58)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             112..789
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(1095..1114)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1179..1198)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3288..3876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3777..3796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5927..5943
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5927..5943
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gatgatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg
       61 gggggcccgg taccagatct ttaaatctag atttaagaag gagatataca tatgagtgcg
      121 attaagccag acatgaagat caaactccgt atggaaggca acgtaaacgg gcaccacttt
      181 gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa
      241 gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac
      301 ggcaacaggg tattcgccaa atatccagac aacatacaag actattttaa gcagtcgttt
      361 cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat ttgcaacgcc
      421 agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt ttatggtacc
      481 aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact
      541 gagaaaatgt atgtgcgtga tggagtgctg acgggtgata ttgagatggc tttgttgctt
      601 gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt
      661 gtcaagttac caggcgccca ctttgtggac cactgcattg agattttaag ccatgacaaa
      721 gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat
      781 gcaagacgag ctgccgttaa atctggcacc aaagctaaac gtgctcagcg tccggcaaaa
      841 tatagctacg ttgacgaaaa cggcgaaact aaaacctgga ctggccaagg ccgtactcca
      901 gctgtaatca aaaaagcaat ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag
      961 caataggcta gccacctgac gtcggggggg gggggaaagc cacgttgtgt ctcaaaatct
     1021 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1081 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctcgag
     1141 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     1201 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagccccatg cgccagagtt
     1261 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     1321 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     1381 tgactagagc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1561 ccccgacgtg gccgaccagc ccgtcatcgt caacgcctga tccgcggtgc ggacaggccg
     1621 tgtcgcgacc ggccgtgcgg aattaagccg gcccgtaccc tgtgaataga ggtccgctgt
     1681 gacacaagaa tccctgttac ttctcgaccg tattgattcg gatgattcct acgcgagcct
     1741 gcggaacgac caggaattct gggagccgct ggcccgccga gccctggagg agctcgggct
     1801 gccggtgccg ccggtgctgc gggtgcccgg cgagagcacc aaccccgtac tggtcggcga
     1861 gcccggcccg gtgatcaagc tgttcggcga gcactggtgc ggtccggaga gcctcgcgtc
     1921 ggagtcggag gcgtacgcgg tcctggcgga cgccccggtg ccggtgcccc gcctcctcgg
     1981 ccgcggcgag ctgcggcccg gcaccggagc ctggccgtgg ccctacctgg tgatgagccg
     2041 gatgaccggc accacctggc ggtccgcgat ggacggcacg accgaccgga acgcgctgct
     2101 cgccctggcc cgcgaactcg gccgggtgct cggccggctg cacagggtgc cgctgaccgg
     2161 gaacaccgtg ctcacccccc attccgaggt cttcccggaa ctgctgcggg aacgccgcgc
     2221 ggcgaccgtc gaggaccacc gcgggtgggg ctacctctcg ccccggctgc tggaccgcct
     2281 ggaggactgg ctgccggacg tggacacgct gctggccggc cgcgaacccc ggttcgtcca
     2341 cggcgacctg cacgggacca acatcttcgt ggacctggcc gcgaccgagg tcaccgggat
     2401 cgtcgacttc accgacgtct atgcgggaga ctcccgctac agcctggtgc aactgcatct
     2461 caacgccttc cggggcgacc gcgagatcct ggccgcgctg ctcgacgggg cgcagtggaa
     2521 gcggaccgag gacttcgccc gcgaactgct cgccttcacc ttcctgcacg acttcgaggt
     2581 gttcgaggag accccgctgg atctctccgg cttcaccgat ccggaggaac tggcgcagtt
     2641 cctctggggg ccgccggaca ccgcccccgg cgcctgacgc cccctctagt aatcttttgc
     2701 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     2761 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     2821 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     2881 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     2941 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     3001 tgacttgacg ggacggcggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca
     3061 gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac
     3121 caactggtcc acctacaaca aagctctcac caaccgtggc tccctcactt tctggctgga
     3181 tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag gcagacctca
     3241 ctagttccat gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3301 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3361 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3421 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3481 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3541 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3601 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3661 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaggggag aaaggcggac
     3721 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3781 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3841 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3901 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3961 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4021 accgagcgca acgcgtgagc ccaccagctc cgtaagttcg ggcgctgtgt ggctcgtacc
     4081 cgcgcattca ggcggcaggg ggtctaacgg gtctaaggcg gcgtgtacgg ccgccacagc
     4141 ggctctcagc ggcccggaaa cgtcctcgaa acgacgcatg tgttcctcct ggttggtaca
     4201 ggtggttggg ggtgctcggc tgtcgctggt gttccaccac cagggctcga cgggagagcg
     4261 ggggagtgtg cagttgtggg gtggcccctc agcgaaatat ctgacttgga gctcgtgtcg
     4321 gaccatacac cggtgattaa tcgtggtcta ctaccaagcg tgagccacgt cgccgacgaa
     4381 tttgagcagc tctggctgcc gtactggccg ctggcaagcg acgatctgct cgaggggatc
     4441 taccgccaaa gccgcgcgtc ggccctaggc cgccggtaca tcgaggcgaa cccaacagcg
     4501 ctggcaaacc tgctggtcgt ggacgtagac catccagacg cagcgctccg agcgctcagc
     4561 gcccgggggt cccatccgct gcccaacgcg atcgtgggca atcgcgccaa cggccacgca
     4621 cacgcagtgt gggcactcaa cgcccctgtt ccacgcaccg aatacgcgcg gcgtaagccg
     4681 ctcgcataca tggcggcgtg cgccgaaggc cttcggcgcg ccgtcgatgg cgaccgcagt
     4741 tactcaggcc tcatgaccaa aaaccccggc cacatcgcct gggaaacgga atggctccac
     4801 tcagatctct acacactcag ccacatcgag gccgagctcg gcgcgaacat gccaccgccg
     4861 cgctggcgtc agcagaccac gtacaaagcg gctccgacgc cgctagggcg gaattgcgca
     4921 ctgttcgatt ccgtcaggtt gtgggcctat cgtcccgccc tcatgcggat ctacctgccg
     4981 acccggaacg tggacggact cggccgcgcg atctatgccg agtgccacgc gcgaaacgcc
     5041 gaatttccgt gcaacgacgt gtgtcccgga ccgctaccgg acagcgaggt ccgcgccatc
     5101 gccaacagca tttggcgttg gatcacaacc aagtcgcgca tttgggcgga cgggatcgtg
     5161 gtctacgagg ccacactcag tgcgcgccag tcggccatct cgcggaaggg cgcagcagcg
     5221 cgcacggcgg cgagcacagt tgcgcggcgc gcaaagtccg cgtcagccat ggaggcattg
     5281 ctatgagcga cggctacagc gacggctaca accggcagcc gactgtccgc aaaaagcggc
     5341 gcgtgaccgc cgccgaaggc gctcgaatca ccggactatc cgaacgccac gtcgtccggc
     5401 tcgtggcgca ggaacgcagc gagtggctcg ccgagcaggc tgcacgccgc gaacgcatcc
     5461 gcgcctatca cgacgacgag ggccactctt ggccgcaaac ggccaaacat ttcgggctgc
     5521 atctggacac cgttaagcga ctcggctatc gggcgaggaa agagcgtgcg gcagaacagg
     5581 aagcggctca aaaggcccac aacgaagccg acaatccacc gctgttctaa cgcaattggg
     5641 gagcgggtgt cgcgggggtt ccgtgggggg ttccgttgca acgggtcgga caggtaaaag
     5701 tcctggtaga cgctagtttt ctggtttggg ccatgcctgt ctcgttgcgt gtttcgttgc
     5761 gcccgttttg aataccagcc agacgagacg gggttctacg aatcttggtc gataccaagc
     5821 catttccgct gaatatcggg gagctcaccg ccagaatcgg tggttgtggt gatgtacgtg
     5881 gcgaactccg ttgtagtgcc tgtggtggca tccgtggcgc ggccgctcta gaactagtgg
     5941 atctgacccg ctccacaacc gctgagcagg gtggcagtca gagcgaccag gccgaccgat
     6001 acgaggcgga ttttgaacag gttcactcga agcctcccgc tgggttcgga gcatcgtcga
     6061 tcacatgacg gcgtgctgac accgattcga ctatgcacag tagtaggtag ccgccgcgga
     6121 cggtagcgca ggcggcctcg gcgccgtcgt ggcgtcgcga tggttggcgc cagacccgcc
     6181 tgatcggcgt cccgaccagg gccgccgggg tggtgagcct cggcttggtc cagccgtagt
     6241 gagacgggat gtcttcccgg ttgtcgggtt tgggtgggcg gtcacatgtt cgctggctgt
     6301 gggcgtgtcg ggggtcgtcg aagcggccgc gaacagggct ggacgatgca cgtcttgcac
     6361 ctcctgtcaa cccccaatgt tgagccgcga tgcccctgac ctgcaccgtt ggaccgcagt
     6421 cgttaggggg gcgtgttagc atggagtgac gaaaggggcc taaatca
//
