LOCUS       sequence-331933-        6044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mEos3.2 in Mycobacterium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Rashid FM, Mahlandt E, van der Vaart M, Boer DEC, Varela Alvarez M, 
            Henneman B, Brocken DJW, Voskamp P, Blok AJ, Shimizu TS, Meijer AH, 
            Luijsterburg MS, Goedhart J, Cremazy FGE, Dame RT
  TITLE     HI-NESS: a family of genetically encoded DNA labels based on a 
            bacterial nucleoid-associated protein.
  JOURNAL   Nucleic Acids Res. 2021 Nov 4. pii: 6414584. doi: 
            10.1093/nar/gkab993.
  PUBMED    34734265
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Nov 4. pii: 6414584. doi: 10.1093/nar/gkab993."
COMMENT     Sequence Label: pRD445
FEATURES             Location/Qualifiers
     source          1..6044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..49)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(34..50)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             104..784
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     rep_origin      2857..3445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3346..3365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5496..5512
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5496..5512
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
       61 ggtaccagat ctttaaatct agatttaaga aggagatata catatgagtg cgattaagcc
      121 agacatgaag atcaaactcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga
      181 cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga
      241 gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag
      301 ggtattcgcc aaatatccag acaacataca agactatttt aagcagtcgt ttcctaaggg
      361 gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcaacg ccagaaacga
      421 cataacaatg gaaggggaca ctttctataa taaagttcga ttttatggta ccaactttcc
      481 cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat
      541 gtatgtgcgt gatggagtgc tgacgggtga tattgagatg gctttgttgc ttgaaggaaa
      601 tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt
      661 accaggcgcc cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa
      721 caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgcaagacg
      781 ataggctagc cactgacgtc ttgtatggga agccccatgc gccagagttg tttctgaaac
      841 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
      901 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gactagagca
      961 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1021 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1081 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgacgtgg
     1141 ccgaccagcc cgtcatcgtc aacgcctgat ccgcggtgcg gacaggccgt gtcgcgaccg
     1201 gccgtgcgga attaagccgg cccgtaccct gtgaatagag gtccgctgtg acacaagaat
     1261 ccctgttact tctcgaccgt attgattcgg atgattccta cgcgagcctg cggaacgacc
     1321 aggaattctg ggagccgctg gcccgccgag ccctggagga gctcgggctg ccggtgccgc
     1381 cggtgctgcg ggtgcccggc gagagcacca accccgtact ggtcggcgag cccggcccgg
     1441 tgatcaagct gttcggcgag cactggtgcg gtccggagag cctcgcgtcg gagtcggagg
     1501 cgtacgcggt cctggcggac gccccggtgc cggtgccccg cctcctcggc cgcggcgagc
     1561 tgcggcccgg caccggagcc tggccgtggc cctacctggt gatgagccgg atgaccggca
     1621 ccacctggcg gtccgcgatg gacggcacga ccgaccggaa cgcgctgctc gccctggccc
     1681 gcgaactcgg ccgggtgctc ggccggctgc acagggtgcc gctgaccggg aacaccgtgc
     1741 tcacccccca ttccgaggtc ttcccggaac tgctgcggga acgccgcgcg gcgaccgtcg
     1801 aggaccaccg cgggtggggc tacctctcgc cccggctgct ggaccgcctg gaggactggc
     1861 tgccggacgt ggacacgctg ctggccggcc gcgaaccccg gttcgtccac ggcgacctgc
     1921 acgggaccaa catcttcgtg gacctggccg cgaccgaggt caccgggatc gtcgacttca
     1981 ccgacgtcta tgcgggagac tcccgctaca gcctggtgca actgcatctc aacgccttcc
     2041 ggggcgaccg cgagatcctg gccgcgctgc tcgacggggc gcagtggaag cggaccgagg
     2101 acttcgcccg cgaactgctc gccttcacct tcctgcacga cttcgaggtg ttcgaggaga
     2161 ccccgctgga tctctccggc ttcaccgatc cggaggaact ggcgcagttc ctctgggggc
     2221 cgccggacac cgcccccggc gcctgacgcc ccctctagta atcttttgcc attctcaccg
     2281 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2341 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2401 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2461 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2521 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2581 gacggcggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc
     2641 ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca
     2701 cctacaacaa agctctcacc aaccgtggct ccctcacttt ctggctggat gatggggcga
     2761 ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcac tagttccatg
     2821 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2881 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2941 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3001 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3061 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3121 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3181 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3241 gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca ggtatccggt
     3301 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3361 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3421 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3481 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3541 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa
     3601 cgcgtgagcc caccagctcc gtaagttcgg gcgctgtgtg gctcgtaccc gcgcattcag
     3661 gcggcagggg gtctaacggg tctaaggcgg cgtgtacggc cgccacagcg gctctcagcg
     3721 gcccggaaac gtcctcgaaa cgacgcatgt gttcctcctg gttggtacag gtggttgggg
     3781 gtgctcggct gtcgctggtg ttccaccacc agggctcgac gggagagcgg gggagtgtgc
     3841 agttgtgggg tggcccctca gcgaaatatc tgacttggag ctcgtgtcgg accatacacc
     3901 ggtgattaat cgtggtctac taccaagcgt gagccacgtc gccgacgaat ttgagcagct
     3961 ctggctgccg tactggccgc tggcaagcga cgatctgctc gaggggatct accgccaaag
     4021 ccgcgcgtcg gccctaggcc gccggtacat cgaggcgaac ccaacagcgc tggcaaacct
     4081 gctggtcgtg gacgtagacc atccagacgc agcgctccga gcgctcagcg cccgggggtc
     4141 ccatccgctg cccaacgcga tcgtgggcaa tcgcgccaac ggccacgcac acgcagtgtg
     4201 ggcactcaac gcccctgttc cacgcaccga atacgcgcgg cgtaagccgc tcgcatacat
     4261 ggcggcgtgc gccgaaggcc ttcggcgcgc cgtcgatggc gaccgcagtt actcaggcct
     4321 catgaccaaa aaccccggcc acatcgcctg ggaaacggaa tggctccact cagatctcta
     4381 cacactcagc cacatcgagg ccgagctcgg cgcgaacatg ccaccgccgc gctggcgtca
     4441 gcagaccacg tacaaagcgg ctccgacgcc gctagggcgg aattgcgcac tgttcgattc
     4501 cgtcaggttg tgggcctatc gtcccgccct catgcggatc tacctgccga cccggaacgt
     4561 ggacggactc ggccgcgcga tctatgccga gtgccacgcg cgaaacgccg aatttccgtg
     4621 caacgacgtg tgtcccggac cgctaccgga cagcgaggtc cgcgccatcg ccaacagcat
     4681 ttggcgttgg atcacaacca agtcgcgcat ttgggcggac gggatcgtgg tctacgaggc
     4741 cacactcagt gcgcgccagt cggccatctc gcggaagggc gcagcagcgc gcacggcggc
     4801 gagcacagtt gcgcggcgcg caaagtccgc gtcagccatg gaggcattgc tatgagcgac
     4861 ggctacagcg acggctacaa ccggcagccg actgtccgca aaaagcggcg cgtgaccgcc
     4921 gccgaaggcg ctcgaatcac cggactatcc gaacgccacg tcgtccggct cgtggcgcag
     4981 gaacgcagcg agtggctcgc cgagcaggct gcacgccgcg aacgcatccg cgcctatcac
     5041 gacgacgagg gccactcttg gccgcaaacg gccaaacatt tcgggctgca tctggacacc
     5101 gttaagcgac tcggctatcg ggcgaggaaa gagcgtgcgg cagaacagga agcggctcaa
     5161 aaggcccaca acgaagccga caatccaccg ctgttctaac gcaattgggg agcgggtgtc
     5221 gcgggggttc cgtggggggt tccgttgcaa cgggtcggac aggtaaaagt cctggtagac
     5281 gctagttttc tggtttgggc catgcctgtc tcgttgcgtg tttcgttgcg cccgttttga
     5341 ataccagcca gacgagacgg ggttctacga atcttggtcg ataccaagcc atttccgctg
     5401 aatatcgggg agctcaccgc cagaatcggt ggttgtggtg atgtacgtgg cgaactccgt
     5461 tgtagtgcct gtggtggcat ccgtggcgcg gccgctctag aactagtgga tctgacccgc
     5521 tccacaaccg ctgagcaggg tggcagtcag agcgaccagg ccgaccgata cgaggcggat
     5581 tttgaacagg ttcactcgaa gcctcccgct gggttcggag catcgtcgat cacatgacgg
     5641 cgtgctgaca ccgattcgac tatgcacagt agtaggtagc cgccgcggac ggtagcgcag
     5701 gcggcctcgg cgccgtcgtg gcgtcgcgat ggttggcgcc agacccgcct gatcggcgtc
     5761 ccgaccaggg ccgccggggt ggtgagcctc ggcttggtcc agccgtagtg agacgggatg
     5821 tcttcccggt tgtcgggttt gggtgggcgg tcacatgttc gctggctgtg ggcgtgtcgg
     5881 gggtcgtcga agcggccgcg aacagggctg gacgatgcac gtcttgcacc tcctgtcaac
     5941 ccccaatgtt gagccgcgat gcccctgacc tgcaccgttg gaccgcagtc gttagggggg
     6001 cgtgttagca tggagtgacg aaaggggcct aaatcagatg atcc
//
