LOCUS       sequence-331947-        9701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral plasmid for GFP-LmnB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pQCXIP-GFP-LmnB1
FEATURES             Location/Qualifiers
     source          1..9701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             585..760
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    823..1022
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1223..1639
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1569..1591
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1607..1623
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1676..1979
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1980..2183
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2180..2204
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2322..2324,2325..2327,2328..3038)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2322..2324,2325..2327,2328..3038)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2367..2388)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2628..2647)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2975..2996
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3048..3072
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4833..4857)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5011..5473
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5067..5084)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5294..5313
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5506..6105
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5506..5525)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6002..6022
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6479..6904
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7007..7029)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7165..7185)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7170..7499
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7350..7485
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7521..7540
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7621..7638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7792..8380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7872..7891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8551..9342,9343..9411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9174..9193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9412..9516)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9584..9602
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcaataa aagagcccac
      601 aacccctcac tcggcgcgcc agtcttccga tagactgcgt cgcccgggta cccgtattcc
      661 caataaagcc tcttgctgtt tgcatccgaa tcgtggtctc gctgttcctt gggagggtct
      721 cctctgagtg attgactacc cacgacgggg gtctttcatt tgggggctcg tccgggattt
      781 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
      841 tctgtgtctg tccgattgtc tagtgtctat gtttgatgtt atgcgcctgc gtctgtacta
      901 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc
      961 cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg
     1021 aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga
     1081 gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg
     1141 cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1201 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     1261 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1321 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1381 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1441 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1501 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1561 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1621 cactccttct ctaggcgccg gaattgaaga tctgggggat cgatcctcta gagtccgtta
     1681 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1741 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1801 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     1861 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1921 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     1981 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     2041 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     2101 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     2161 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2221 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccgggaac
     2281 ggtgcattgg aacggacctg caggaattga tccgcggccg catggtgagc aagggcgagg
     2341 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2401 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2461 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2521 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2581 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2641 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2701 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2761 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2821 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2881 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2941 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3001 ccgccgggat cactctcggc atggacgagc tgtacaagac cggatcaaca agtttgtaca
     3061 aaaaagcagg ctgcgcgact gcgacccccg tgccgccgcg gatgggcagc cgcgctggcg
     3121 gccccaccac gccgctgagc cccacgcgcc tgtcgcggct ccaggagaag gaggagctgc
     3181 gcgagctcaa tgaccggctg gcggtgtaca tcgacaaggt gcgcagcctg gagacggaga
     3241 acagcgcgct gcagctgcag gtgacggagc gcgaggaggt gcgcggccgt gagctcaccg
     3301 gcctcaaggc gctctacgag accgagctgg ccgacgcgcg acgcgcgctc gacgacacgg
     3361 cccgcgagcg cgccaagctg cagatcgagc tgggcaagtg caaggcggaa cacgaccagc
     3421 tgctcctcaa ctatgctaag aaggaatctg atcttaatgg cgcccagatc aagcttcgag
     3481 aatatgaagc agcactcaat tcgaaagatg cagctcttgc tactgcactt ggtgacaaaa
     3541 aaagtttaga gggagatttg gaggatctga aggatcagat tgcccagttg gaagcctcct
     3601 tagctgcagc caaaaaacag ttagcagatg aaactttact taaagtagat ttggagaatc
     3661 gttgtcagag ccttactgag gacttggagt ttcgcaaaag catgtatgaa gaggagatta
     3721 acgagaccag aaggaagcat gaaacgcgct tggtagaggt ggattctggg cgtcaaattg
     3781 agtatgagta caagctggcg caagcccttc atgagatgag agagcaacat gatgcccaag
     3841 tgaggctgta taaggaggag ctggagcaga cttaccatgc caaacttgag aatgccagac
     3901 tgtcatcaga gatgaatact tctactgtca acagtgccag ggaagaactg atggaaagcc
     3961 gcatgagaat tgagagcctt tcatcccagc tttctaatct acagaaagag tctagagcat
     4021 gtttggaaag gattcaagaa ttagaggact tgcttgctaa agaaaaagac aactctcgtc
     4081 gcatgctgac agacaaagag agagagatgg cggaaataag ggatcaaatg cagcaacagc
     4141 tgaatgacta tgaacagctt cttgatgtaa agttagccct ggacatggaa atcagtgctt
     4201 acaggaaact cttagaaggc gaagaagaga ggttgaagct gtctccaagc ccttcttccc
     4261 gtgtgacagt atcccgagca tcctcaagtc gtagtgtacg tacaactaga ggaaagcgga
     4321 agagggttga tgtggaagaa tcagaggcga gtagtagtgt tagcatctct cattccgcct
     4381 cagccactgg aaatgtttgc atcgaagaaa ttgatgttga tgggaaattt atccgcttga
     4441 agaacacttc tgaacaggat caaccaatgg gaggctggga gatgatcaga aaaattggag
     4501 acacatcagt cagttataaa tatacctcaa gatatgtgct gaaggcaggc cagactgtta
     4561 caatttgggc tgcaaacgct ggtgtcacag ccagcccccc aactgacctc atctggaaga
     4621 accagaactc gtggggcact ggcgaagatg tgaaggttat attgaaaaat tctcagggag
     4681 aggaggttgc tcaaagaagt acagtcttta aaacaaccat acctgaagaa gaggaggagg
     4741 aggaagaagc agctggagtg gttgttgagg aagaactttt ccaccagcag ggaaccccaa
     4801 gagcatccaa tagaagctgt gcaattatgt agacccagct ttcttgtaca aagtggttga
     4861 tccggtaggc ctcgtacgct taattaacgg atccggaatt ccgtccccct cccccccccc
     4921 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     4981 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     5041 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     5101 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     5161 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     5221 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     5281 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     5341 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     5401 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     5461 aacacgatga taagcttgcc acaacccaca aggagacgac cttccatgac cgagtacaag
     5521 cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc
     5581 gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg
     5641 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5701 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5761 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5821 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5881 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5941 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6001 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6061 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     6121 ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct ccgaccgaag ccacccgggg
     6181 cggccccgcc gaccccgcac ccgcccccga ggcccaccga ctctagtcga gggctgcagc
     6241 gctgcagagg ccgagtgcag aactgctcca aagggacctc aaggctttcc gagggacact
     6301 aggctgactc catcgagcca gtgtagagat aagcttatcg attagtccaa tttgttaaag
     6361 acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta
     6421 tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     6481 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     6541 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     6601 ggtcgaccct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     6661 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     6721 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     6781 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     6841 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     6901 catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg
     6961 ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     7021 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     7081 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     7141 atagcggagt gtagatccgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     7201 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     7261 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7321 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7381 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7441 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7501 agcttactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     7561 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     7621 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7681 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7741 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7801 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7861 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7921 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7981 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8041 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8101 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8161 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8221 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8281 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8341 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8401 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8461 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8521 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8581 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8641 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8701 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8761 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8821 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     8881 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8941 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9001 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9061 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9121 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     9181 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9241 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9301 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9361 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9421 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9481 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9541 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     9601 gccctttcgt cttcaagaat tagcttggcc attgcatacg ttgtatccat atcataatat
     9661 gtacatttat attggctcat gtccaacatt accgccatgt t
//
