LOCUS       sequence-331950-       11228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of SARS-CoV-2 soluble spike trimer protein 
            (D614G mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, 
            Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
  TITLE     Optimizing high-yield production of SARS-CoV-2 soluble spike trimers
            for serology assays.
  JOURNAL   Protein Expr Purif. 2020 Jun 4;174:105686. doi: 
            10.1016/j.pep.2020.105686.
  PUBMED    32504802
REFERENCE   2  (bases 1 to 11228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2020-06-4"; volume: "174:105686. doi"; pages: "
            10.1016/j.pep.2020.105686"
COMMENT     Sequence Label: R619-M64-303: CMV51p> SARS-CoV-2
            S(1-1208)-2P-...
FEATURES             Location/Qualifiers
     source          1..11228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      203..1992
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(2029..2048)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2198..2218
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2229..2532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2533..2736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2687..2707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2733..2757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2802..2826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6549..6572
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6576..6599
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6606..6629
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6669..6692
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6694..6718)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6780..6798)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6781..6798)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6818..6835)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6824..7048
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7094..7522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7181..7200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7391..7412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7531..7551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7536..7865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7716..7851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7778..7797
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7932..8726
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7986..8005)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8596..8615
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8900..9021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8937..8956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8991..9010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9070..9086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9070..9086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9083..9105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9094..9110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9118..9124,9125..9142,9143..9148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9163..9184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9301..9318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9472..10060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9552..9571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10231..11022,11023..11091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10854..10873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11092..11196)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatccgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
       61 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
      121 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
      181 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
      241 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
      301 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
      361 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
      421 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
      481 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
      541 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
      601 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
      661 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
      721 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
      781 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
      841 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
      901 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
      961 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     1021 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     1081 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     1141 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     1201 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     1261 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     1321 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     1381 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     1441 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     1501 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     1561 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     1621 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     1681 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     1741 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     1801 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     1861 cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt
     1921 gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt
     1981 gtccttttcc tgctggggat ctcccgatcc cctatggtgc actctcagta caatctgctc
     2041 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     2101 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     2161 tctgcttagg gttaggcgtt ttgcgctgct tcgatcacaa ctttgtatag aaaagttggc
     2221 cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2281 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2341 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2401 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2461 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     2521 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
     2581 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2641 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     2701 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2761 tggagacgcc atccacgctg ttttgacctc catagaagac aacaactttg tacaaaaaag
     2821 ttggcaccat gttcgtgttc cttgtcctcc tgccgctggt gtcatcgcag tgcgtgaacc
     2881 tgaccacccg gactcagctg cctcctgctt acactaactc cttcactcgc ggggtgtact
     2941 accctgacaa agtgttccgg tcgtccgtgc tgcatagcac ccaggacctg ttccttccgt
     3001 tcttctccaa cgtgacctgg ttccacgcca ttcacgtgtc ggggaccaac ggcactaaga
     3061 gattcgacaa ccccgtcttg cccttcaatg acggagtata cttcgcctcg acggaaaagt
     3121 ccaacatcat tcggggttgg atcttcggca ctaccctgga cagcaagacc cagtccctgc
     3181 tcattgtcaa caacgccacc aacgtcgtga tcaaagtctg cgagtttcag ttttgtaacg
     3241 atccgttcct cggcgtgtac taccacaaga acaacaagag ctggatggag tccgaattcc
     3301 gggtctactc ctccgccaac aactgtacct tcgaatacgt gtcccaacct ttcctcatgg
     3361 acctcgaagg aaaacaggga aacttcaaga acttgagaga gtttgtgttc aagaatatcg
     3421 atggctactt caagatctac tcgaagcata ccccgatcaa cctcgtgcgg gacctacctc
     3481 aagggttctc cgcacttgag ccgctcgtgg acctccccat tggcatcaac atcaccagat
     3541 tccagacttt actggcactg catagatcgt atctcacacc cggggactcc tcctccgggt
     3601 ggacagctgg tgctgcggcc tactacgtgg gatatctcca gccccggacc ttcttgctga
     3661 agtacaacga gaacggcacc atcacggatg ccgtggactg tgcactggac cccttgagtg
     3721 aaaccaagtg cacgctgaag tccttcaccg tggaaaaagg catctaccag accagcaact
     3781 tcagggtgca gcctactgaa tcaatcgtgc ggttccctaa cattaccaat ctgtgcccgt
     3841 tcggcgaagt gttcaacgcc actagattcg cctcggtgta cgcctggaac cgaaagcgga
     3901 tcagcaactg cgtggccgac tactcagtgc tctacaactc ggccagcttc tccaccttca
     3961 agtgctacgg agtgtcgccc accaagctga acgatctgtg cttcaccaac gtgtacgcgg
     4021 attccttcgt gatccgcgga gatgaagtcc gccaaatcgc ccccggtcaa accggaaaga
     4081 ttgccgacta caactataag ctgccggacg actttaccgg atgcgtgatt gcctggaatt
     4141 ccaacaattt ggattcaaaa gtcggcggca actacaacta cctgtaccgc ctgttccgga
     4201 agtcaaacct gaagccgttc gaacgcgaca tcagcaccga aatctatcaa gcgggaagca
     4261 ctccctgcaa cggggtggag ggcttcaact gctacttccc gctgcaaagc tacggattcc
     4321 agcctaccaa cggcgttgga taccagccat atcgcgtggt cgtgctctcc tttgaacttc
     4381 tgcacgcccc tgccactgtg tgcggcccca agaagtccac taacctcgtc aagaataagt
     4441 gcgtgaattt caactttaac ggattgaccg ggaccggagt gctcaccgag tccaacaaga
     4501 aattcctgcc gttccaacag tttggacggg acatcgccga tactacggat gctgtgcggg
     4561 acccacagac tctggagatc ctggacatta ctccctgttc ctttggcggg gtgtcggtca
     4621 tcacccctgg tactaacacc agcaaccagg tcgccgtgct gtatcagggg gtgaactgca
     4681 ccgaagtgcc cgtggcgatc cacgccgacc agctgactcc aacttggcgc gtgtactcga
     4741 ctggttcgaa cgtgttccag actcgggccg gttgcctgat cggagcggag catgtcaaca
     4801 acagctacga gtgcgacatc cctattggag ccgggatttg cgccagctac cagacccaga
     4861 ccaattcgcc tgggtcggcc agcagcgtgg cttcccaatc gatcatcgcc tacaccatgt
     4921 ccctgggagc cgagaactca gtggcgtact ccaacaactc gattgccatc cccactaatt
     4981 tcacgatcag cgtgaccacc gagatcctgc ctgtgtccat gaccaagacc tccgtggact
     5041 gcaccatgta catctgcgga gactccaccg agtgctccaa cctcctgctg caatacggct
     5101 cgttctgtac ccaactgaac cgagccctga ctgggatcgc cgtggagcag gacaagaaca
     5161 cacaggaggt gttcgcacaa gtcaagcaga tctacaagac cccgcccatc aaggatttcg
     5221 gagggttcaa tttctcccaa attctgccgg acccttccaa gccgtccaag aggtcattca
     5281 ttgaggatct cctgttcaac aaggtcaccc tggccgatgc tggtttcatc aagcagtacg
     5341 gagactgcct gggggacatc gcagcccgcg atctgatctg cgcccaaaag ttcaacggcc
     5401 tgaccgtcct gcctccgctt ctgactgatg aaatgattgc ccagtacacc tctgcgctgc
     5461 tggctggcac tatcacctcc ggctggacct tcggcgctgg agcagcactg cagattccat
     5521 ttgccatgca aatggcctac cgcttcaacg gaatcggagt gactcagaac gtcctgtacg
     5581 agaaccagaa gctcatcgcc aaccagttca actccgccat tgggaagatt caggattccc
     5641 tgtcgagcac tgcgtcagcc ctgggaaagc tgcaggacgt cgtgaaccag aacgcacagg
     5701 ccctgaacac ccttgtgaag cagctgtcgt cgaacttcgg ggcgatctcc tccgtgttga
     5761 acgacatcct gtcccggctg gacccgcctg aggcagaagt gcagatcgac cggctgatta
     5821 ccggacgcct ccagtccctt caaacttatg tgacccagca gctgattcgc gcggcggaaa
     5881 ttcgggctag cgccaatctg gctgccacca agatgagcga atgcgtgctt ggacagtcca
     5941 agagagtgga cttttgtgga aagggctacc acctgatgag cttcccccaa tccgccccac
     6001 acggtgtcgt gtttctgcac gtgacctacg tgccggcgca ggaaaagaac ttcaccactg
     6061 cccctgccat ctgccacgat gggaaggcgc acttcccgag ggagggagtg ttcgtgtcaa
     6121 acggaaccca ctggttcgtg acccaaagga acttctacga gccccagatt atcaccaccg
     6181 ataacacttt cgtgagcggc aactgcgacg tggtcatcgg catcgtgaac aacactgtgt
     6241 acgacccgct gcaaccggag ctcgatagct ttaaggaaga actcgacaag tactttaaga
     6301 atcatacgtc ccccgatgtg gatctgggcg acattagcgg tatcaacgcg tctgtcgtga
     6361 acattcagaa ggagattgac aggctcaacg aagtcgccaa aaacctgaac gagagcctga
     6421 tagacttgca ggaactggga aaatacgagc agggctccgg ttacatcccc gaagctccac
     6481 gggacggaca ggcctacgtg cgcaaggacg gagaatgggt gctgctgagc accttcctcg
     6541 ggcggtccct ggaggtgctg ttccaaggcc caggccacca tcatcatcac caccaccact
     6601 cggcttggtc acacccccaa ttcgaaaagg gcggtggttc tggaggaggc ggatccggag
     6661 gatccgcgtg gagtcatccg cagtttgaaa agtaatcaac tttcttgtac aaagttgtga
     6721 aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga gggccctatt
     6781 ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     6841 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6901 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6961 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7021 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     7081 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7141 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7201 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7261 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7321 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7381 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7441 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     7501 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     7561 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7621 ggtgtggaaa gtccctaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7681 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7741 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7801 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     7861 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     7921 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     7981 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     8041 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     8101 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     8161 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     8221 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     8281 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     8341 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     8401 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     8461 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     8521 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     8581 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     8641 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     8701 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     8761 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     8821 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     8881 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     8941 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9001 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     9061 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9121 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     9241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     9301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    10081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    10141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    10201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    10321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    10441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    11101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    11161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    11221 tgacgtcg
//
