LOCUS       sequence-331953-        3446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SARS-CoV-2 ORF8 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3446)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046811
FEATURES             Location/Qualifiers
     source          1..3446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             320..340
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             341..370
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     rep_origin      491..1079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     980..999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1185..1844
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1251..1270)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1915..1949
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     1977..1996
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1977..1995
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1996..2020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2035..2057
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctttggggaa attatgaggg gatctctcag tactcaacat caaccatatg tagttgatga
       61 cccgtgtcct attcacttct attctaaatg gtatattaga gtaggagcta gaaaatcagc
      121 acctttaatt gaattgtgcg tggatgaggc tggttctaaa tcacccattc agtacatcga
      181 tatcggtaat tatacagttt cctgtttacc ttttacaatt aattgccagg aacctaaatt
      241 gggtagtctt gtagtgcgtt gttcgttcta tgaagacttt ttagagtatc atgacgttcg
      301 tgttgtttta gatttcatcg agaacctgta ctttcaaagc gagcagaagc ttatttccga
      361 agaagatctc taaccggctt atcggtcagt ttcacctgat ttacgtaaaa acccgcttcg
      421 gcgggttttt gcttttggag gggcagaaag atgaatgact gtccacgacg ctatacccaa
      481 aagaaaaagc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      541 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      601 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      661 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      721 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      781 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      841 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      901 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      961 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1021 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaat
     1081 tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     1141 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     1201 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     1261 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     1321 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     1381 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     1441 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     1501 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     1561 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgttt
     1621 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1681 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1741 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     1801 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg
     1861 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     1921 acgctcggtt gccgccgggc gttttttatt ggtcatcgat ctcgatcccg cgaaattaat
     1981 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
     2041 taactttaag aaggagactc ttctatgaaa tttcttgttt tcttaggaat catcacaact
     2101 gtagctgcat ttcaccaaga atgtagttta cagtcatgta ctcaacactg atgaatcccc
     2161 taatgatttt krtmaaaatc attaagttaa ggtggataca catcttgtca tatgatcaaa
     2221 tggtttcgcg aaaaatcaat aatcagacaa caagatgtgc gaactcgata ttttacacga
     2281 ctctctttac caattctgcc ccgaattaca cttaaaacga ctcaacagct taacgttggc
     2341 ttgccacgca ttacttgact gtaaaactct cactcttacc gaacttggcc gtaacctgcc
     2401 aaccaaagcg agaacaaaac ataacatcaa acgaatcgac cgattgttag gtaatcgtca
     2461 cctccacaaa gagcgactcg ctgtataccg ttggcatgct agctttatct gttcgggcaa
     2521 tacgatgccc attgtacttg ttgactggtc tgatattcgt gagcaaaaac gacttatggt
     2581 attgcgagct tcagtcgcac tacacggtcg ttctgttact ctttatgaga aagcgttccc
     2641 gctttcagag caatgttcaa agaaagctca tgaccaattt ctagccgacc ttgcgagcat
     2701 tctaccgagt aacaccacac cgctcattgt cagtgatgct ggctttaaag tgccatggta
     2761 taaatccgtt gagaagctgg gttggtactg gttaagtcga gtaagaggaa aagtacaata
     2821 tgcagaccta ggagcggaaa actggaaacc tatcagcaac ttacatgata tgtcatctag
     2881 tcactcaaag actttaggct ataagaggct gactaaaagc aatccaatct catgccaaat
     2941 tctattgtat aaatctcgct ctaaaggccg aaaaaatcag cgctcgacac ggactcattg
     3001 tcaccacccg tcacctaaaa tctactcagc gtcggcaaag gagccatggg ttctagcaac
     3061 taacttacct gttgaaattc gaacacccaa acaacttgtt aatatctatt cgaagcgaat
     3121 gcagattgaa gaaaccttcc gagacttgaa aagtcctgcc tacggactag gcctacgcca
     3181 tagccgaacg agcagctcag agcgttttga tatcatgctg ctaatcgccc tgatgcttca
     3241 actaacatgt tggcttgcgg gcgttcatgc tcagaaacaa ggttgggaca agcacttcca
     3301 ggctaacaca gtcagaaatc gaaacgtact ctcaacagtt cgcttaggca tggaagtttt
     3361 gcggcattct ggctacacaa taacaaggga agacttactc gtggctgcaa ccctactagc
     3421 tcaaaattta ttcacacatg gttacg
//
