LOCUS       sequence-331955-        8226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral plasmid for GFP-BAF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pQCXIP-GFP-BAF
FEATURES             Location/Qualifiers
     source          1..8226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1034..1052
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        1152..1531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1532..1735
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1736..1911
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    1974..2173
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2374..2790
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2720..2742
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2758..2774
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        2827..3130
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3131..3334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3331..3355
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3473..3475,3476..3478,3479..4189)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3473..3475,3476..3478,3479..4189)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3518..3539)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3779..3798)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4126..4147
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4199..4223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4496..4520)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(4576..5150,5151..5153,5154..5162)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4743..4760)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4970..4989
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5182..5781
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5182..5201)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5678..5698
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6155..6580
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6683..6705)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6841..6861)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6846..7175
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7026..7161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7197..7216
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7297..7314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7468..8056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7548..7567)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1021 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tagcttggcc
     1081 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
     1141 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     1201 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     1261 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     1321 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1381 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     1441 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1501 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     1561 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1621 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1681 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcaata
     1741 aaagagccca caacccctca ctcggcgcgc cagtcttccg atagactgcg tcgcccgggt
     1801 acccgtattc ccaataaagc ctcttgctgt ttgcatccga atcgtggtct cgctgttcct
     1861 tgggagggtc tcctctgagt gattgactac ccacgacggg ggtctttcat ttgggggctc
     1921 gtccgggatt tggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg
     1981 ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgtttgatgt tatgcgcctg
     2041 cgtctgtact agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag
     2101 ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg actttggggg ccgtttttgt
     2161 ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg
     2221 gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg
     2281 aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt
     2341 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
     2401 ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc
     2461 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     2521 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     2581 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     2641 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct
     2701 ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt
     2761 tatccagccc tcactccttc tctaggcgcc ggaattgaag atctggggga tcgatcctct
     2821 agagtccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2881 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2941 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     3001 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     3061 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     3121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     3181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     3241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     3301 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     3361 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
     3421 cggccgggaa cggtgcattg gaacggacct gcaggaattg atccgcggcc gcatggtgag
     3481 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3541 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3601 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3661 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3721 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3781 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3841 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3901 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     3961 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     4021 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4081 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4141 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga ccggatcaac
     4201 aagtttgtac aaaaaagcag gctgcatgac aacctcccaa aagcaccgag acttcgtggc
     4261 agagcccatg ggggagaagc cagtggggag cctggctggg attggtgaag tcctgggcaa
     4321 gaagctggag gaaaggggtt ttgacaaggc ctatgttgtc cttggccagt ttctggtgct
     4381 aaagaaagat gaagacctct tccgggaatg gctgaaagac acttgtggcg ccaacgccaa
     4441 gcagtcccgg gactgcttcg gatgccttcg agagtggtgc gacgccttct tgtgaaccca
     4501 gctttcttgt acaaagtggt tgatccggta ggcctcgtac gcttaattaa cggatccgga
     4561 attccgcccc ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     4621 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4681 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4741 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4801 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4861 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4921 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4981 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5041 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     5101 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa
     5161 cccacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5221 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5281 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5341 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5401 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5461 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5521 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     5581 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     5641 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     5701 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     5761 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     5821 gcacgacccc atggctccga ccgaagccac ccggggcggc cccgccgacc ccgcacccgc
     5881 ccccgaggcc caccgactct agtcgagggc tgcagcgctg cagaggccga gtgcagaact
     5941 gctccaaagg gacctcaagg ctttccgagg gacactaggc tgactccatc gagccagtgt
     6001 agagataagc ttatcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc
     6061 tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat
     6121 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     6181 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
     6241 agaagttcag atcaaggtca ggaacagatg gaacagggtc gaccctagag aaccatcaga
     6301 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
     6361 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     6421 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
     6481 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     6541 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
     6601 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc tgcctcgcgc
     6661 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6721 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     6781 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtag atccggctgt
     6841 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6901 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6961 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     7021 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7081 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7141 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tactggctta actatgcggc
     7201 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     7261 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7321 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7381 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7441 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7501 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7801 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7861 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7921 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7981 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8041 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8101 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8161 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8221 tgacag
//
