LOCUS       sequence-331961-        5142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR gateway shuttling vector with DPP9S (no stop codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pDONR221 DPP9S
FEATURES             Location/Qualifiers
     source          1..5142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(136..163)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(255..341)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     390..412
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     404..421
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     405..421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    437..536
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      539..548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3135..3234)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3251..3270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3252..3270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3275..3291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3275..3291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3393..3412)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3404..4213
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3471..3490)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4360..4948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4849..4868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5102..5119
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
       61 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
      121 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
      181 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
      241 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
      301 cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac
      361 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag
      421 tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca
      481 acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag caggcttcgc
      541 caccatggcc accaccggga ccccaacggc cgaccgaggc gacgcagccg ccacagatga
      601 cccggccgcc cgcttccagg tgcagaagca ctcgtgggac gggctccgga gcatcatcca
      661 cggcagccgc aagtactcgg gcctcattgt caacaaggcg ccccacgact tccagtttgt
      721 gcagaagacg gatgagtctg ggccccactc ccaccgcctc tactacctgg gaatgccata
      781 tggcagccga gagaactccc tcctctactc tgagattccc aagaaggtcc ggaaagaggc
      841 tctgctgctc ctgtcctgga agcagatgct ggatcatttc caggccacgc cccaccatgg
      901 ggtctactct cgggaggagg agctgctgag ggagcggaaa cgcctggggg tcttcggcat
      961 cacctcctac gacttccaca gcgagagtgg cctcttcctc ttccaggcca gcaacagcct
     1021 cttccactgc cgcgacggcg gcaagaacgg cttcatggtg tcccctatga aaccgctgga
     1081 aatcaagacc cagtgctcag ggccccggat ggaccccaaa atctgccctg ccgaccctgc
     1141 cttcttctcc ttcatcaata acagcgacct gtgggtggcc aacatcgaga caggcgaaga
     1201 gcggcggctg accttctgcc accaaggttt atccaatgtc ctggatgacc ccaagtctgc
     1261 gggtgtggcc accttcgtca tacaggaaga gttcgaccgc ttcactgggt actggtggtg
     1321 ccccacagcc tcctgggaag gttcagaggg cctcaagacg ctgcgaatcc tgtatgagga
     1381 agtcgatgag tccgaggtgg aggtcattca cgtcccctct cctgcgctag aagaaaggaa
     1441 gacggactcg tatcggtacc ccaggacagg cagcaagaat cccaagattg ccttgaaact
     1501 ggctgagttc cagactgaca gccagggcaa gatcgtctcg acccaggaga aggagctggt
     1561 gcagcccttc agctcgctgt tcccgaaggt ggagtacatc gccagggccg ggtggacccg
     1621 ggatggcaaa tacgcctggg ccatgttcct ggaccggccc cagcagtggc tccagctcgt
     1681 cctcctcccc ccggccctgt tcatcccgag cacagagaat gaggagcagc ggctagcctc
     1741 tgccagagct gtccccagga atgtccagcc gtatgtggtg tacgaggagg tcaccaacgt
     1801 ctggatcaat gttcatgaca tcttctatcc cttcccccaa tcagagggag aggacgagct
     1861 ctgctttctc cgcgccaatg aatgcaagac cggcttctgc catttgtaca aagtcaccgc
     1921 cgttttaaaa tcccagggct acgattggag tgagcccttc agccccgggg aagatgaatt
     1981 taagtgcccc attaaggaag agattgctct gaccagcggt gaatgggagg ttttggcgag
     2041 gcacggctcc aagatctggg tcaatgagga gaccaagctg gtgtacttcc agggcaccaa
     2101 ggacacgccg ctggagcacc acctctacgt ggtcagctat gaggcggccg gcgagatcgt
     2161 acgcctcacc acgcccggct tctcccatag ctgctccatg agccagaact tcgacatgtt
     2221 cgtcagccac tacagcagcg tgagcacgcc gccctgcgtg cacgtctaca agctgagcgg
     2281 ccccgacgac gaccccctgc acaagcagcc ccgcttctgg gctagcatga tggaggcagc
     2341 cagctgcccc ccggattatg ttcctccaga gatcttccat ttccacacgc gctcggatgt
     2401 gcggctctac ggcatgatct acaagcccca cgccttgcag ccagggaaga agcaccccac
     2461 cgtcctcttt gtatatggag gcccccaggt gcagctggtg aataactcct tcaaaggcat
     2521 caagtacttg cggctcaaca cactggcctc cctgggctac gccgtggttg tgattgacgg
     2581 caggggctcc tgtcagcgag ggcttcggtt cgaaggggcc ctgaaaaacc aaatgggcca
     2641 ggtggagatc gaggaccagg tggagggcct gcagttcgtg gccgagaagt atggcttcat
     2701 cgacctgagc cgagttgcca tccatggctg gtcctacggg ggcttcctct cgctcatggg
     2761 gctaatccac aagccccagg tgttcaaggt ggccatcgcg ggtgccccgg tcaccgtctg
     2821 gatggcctac gacacagggt acactgagcg ctacatggac gtccctgaga acaaccagca
     2881 cggctatgag gcgggttccg tggccctgca cgtggagaag ctgcccaatg agcccaaccg
     2941 cttgcttatc ctccacggct tcctggacga aaacgtgcac tttttccaca caaacttcct
     3001 cgtctcccaa ctgatccgag cagggaaacc ttaccagctc cagatctacc ccaacgagag
     3061 acacagtatt cgctgccccg agtcgggcga gcactatgaa gtcacgttgc tgcactttct
     3121 acaggaatac ctcgacccag ctttcttgta caaagttggc attataagaa agcattgctt
     3181 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     3241 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     3301 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     3361 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     3421 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     3481 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     3541 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     3601 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     3661 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     3721 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     3781 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     3841 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     3901 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3961 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     4021 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     4081 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     4141 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     4201 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     4261 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     4321 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4381 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4441 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4501 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4561 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4621 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4681 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4741 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4801 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4861 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4921 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4981 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5041 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5101 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cg
//
