LOCUS       sequence-331967-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway attB2r/attB3 entry clone containing a polyA sequence for 
            transcription termination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D
  TITLE     Combinatorial assembly of clone libraries using site-specific 
            recombination.
  JOURNAL   Methods Mol Biol. 2014;1116:193-208. doi: 
            10.1007/978-1-62703-764-8_14.
  PUBMED    24395366
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2014"; volume: "1116:193-208. doi"; pages: " 
            10.1007/978-1-62703-764-8_14"
COMMENT     Sequence Label: C238-E07: attB2r-SV40polyA-attB3
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(42..165)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    296..430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(333..352)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     387..406
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(432..527)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cgagnnccta caggtcacta ataccatcta agtagttgat tcatagtgac tggatatgtt
       61 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
      121 ttatatcacc ttacgtttct cgttcagctt tcttgtacaa agtgggcagc ttgtcgagaa
      181 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
      241 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      301 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      361 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      421 tgtctggatc tcaactttat tatacaaagt tggcattata ggaaagcatt gcttatcaat
      481 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgca tgcaagctta
      541 gatcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
      601 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
      661 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
      721 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact ggggtacagt
      781 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
      841 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
      901 gggaagcggt gatcgccgaa gtatc
//
