LOCUS       sequence-33197-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Takagi J, Erickson HP, Springer TA
  TITLE     C-terminal opening mimics 'inside-out' activation of integrin 
            alpha5beta1.
  JOURNAL   Nat Struct Biol. 2001 May . 8(5):412-6.
  PUBMED    11323715
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol. 2001 May . 8(5):412-6."
COMMENT     Sequence Label: Integrin alpha5-AHCys
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        73..269
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      120..255
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             285..553
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
ORIGIN
        1 attagcttgg tactaatacg actcactata gggagaccca agctggctag gtaagcttgg
       61 taccacgcat gctgcatctc aattagtcag caaccatagt cccgccccta actccgccca
      121 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
      181 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
      241 gcttttttgg aggcctaggc ttttgcaaaa agctcctcga tcgaggggct cgcatctctc
      301 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
      361 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
      421 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
      481 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
      541 gccgttacag atccaagctc ctcgacgaag agcgggcgct atggggagcc ggacgccaga
      601 gtcccctctc cacgccgtgc agctgcgctg gggcccccgg cgccgacccc cgctgctgcc
      661 gctgctgttg ctgctgctgc cgccgccacc cagggtcggg ggcttcaact tagacgcgga
      721 ggccccagca gtactctcgg ggcccccggg ctccttcttc ggattctcag tggagtttta
      781 ccggccggga acagacgggg tcagtgtgct ggtgggagca cccaaaggct aataccagcc
      841 agccaggagt gctgcag
//
