LOCUS       sequence-332009-        7064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing CD47 in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Wang SK, Xue Y, Cepko CL
  TITLE     Augmentation of CD47/SIRPalpha signaling protects cones in genetic 
            models of retinal degeneration.
  JOURNAL   JCI Insight. 2021 Aug 23;6(16). pii: e150796. doi: 
            10.1172/jci.insight.150796.
  PUBMED    34197341
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2021-08-23"; volume: "6(16). pii: e150796. doi"; 
            pages: " 10.1172/jci.insight.150796"
COMMENT     Sequence Label: AAV-hRedOpsin-CD47
FEATURES             Location/Qualifiers
     source          1..7064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   20..149
                     /label=AAV2 ITR
     primer_bind     complement(3336..3356)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3754..3765)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3832..3849)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3838..4045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4133..4262
                     /label=AAV2 ITR
     primer_bind     complement(4281..4298)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4281..4297)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4290..4312)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4439..4894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4526..4545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4736..4757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4920..5024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5025..5093,5094..5885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5243..5262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6056..6644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6545..6564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6932..6953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6968..6973,6974..6991,6992..6998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7006..7022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7011..7033
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7030..7046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7030..7046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
       61 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      121 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      181 atctacgtag ccatgctcta ggaagatcgg aattcgccct taagctagcc tacagcagcc
      241 agggtgagat tatgaggctg agctgagaat atcaagactg taccgagtag ggggccttgg
      301 caagtgtgga gagcccggca gctggggcag agggcggagt acggtgtgcg tttacggacc
      361 tcttcaaacg aggtaggaag gtcagaagtc aaaaagggaa caaatgatgt ttaaccacac
      421 aaaaatgaaa atccaatggt tggatatcca ttccaaatac acaaaggcaa cggataagtg
      481 atccgggcca ggcacagaag gccatgcacc cgtaggattg cactcagagc tcccaaatgc
      541 ataggaatag aagggtgggt gcaggaggct gaggggtggg gaaagggcat gggtgtttca
      601 tgaggacaga gcttccgttt catgcaatga aaagagtttg gagacggatg gtggtgactg
      661 gactatacac ttacacacgg tagcgatggt acactttgta ttatgtatat tttaccacga
      721 tctttttaaa gtgtcaaagg caaatggcca aatggttcct tgtcctatag ctgtagcagc
      781 catcggctgt tagtgacaaa gcccctgagt caagatgaca gcagccccca taactcctaa
      841 tcggctctcc cgcgtggagt catttaggag tagtcgcatt agagacaagt ccaacatcta
      901 atcttccacc ctggccaggg ccccagctgg cagcgagggt gggagactcc gggcagagca
      961 gagggcgctg acattggggc ccggcctggc ttgggtccct ctggcctttc cccaggggcc
     1021 ctctttcctt ggggctttct tgggccgcca ctgctcccgc tcctctcccc ccatcccacc
     1081 ccctcacccc ctcgttcttc atatccttct ctagtgctcc ctccactttc atccaccctt
     1141 ctgcaagagt gtgggaccac aaatgagttt tcacctggcc tggggacaca cgtgccccca
     1201 caggtgctga gtgactttct aggacagtaa tctgctttag gctaaaatgg gacttgatct
     1261 tctgttagcc ctaatcatca attagcagag ccggtgaagg tgcagaacct accgcctttc
     1321 caggcctcct cccacctctg ccacctccac tctccttcct gggatgtggg ggctggcaca
     1381 cgtgtggccc agggcattgg tgggattgca ctgagctggg tcattagcgt aatcctggac
     1441 aagggcagac agggcgagcg gagggccagc tccggggctc aggcaaggct gggggcttcc
     1501 cccagacacc ccactcctcc tctgctggac ccccacttca tagggcactt cgtgttctca
     1561 aagggcttcc aaatagcatg gtggccttgg atgcccaggg aagcctcaga gttgcttatc
     1621 tccctctaga cagaagggga atctcggtca agagggagag gtcgccctgt tcaaggccac
     1681 ccagccagct catggcggta atgggacaag gctggccagc catcccaccc tcagaaggga
     1741 cccggtgggg caggtgatct cagaggaggc tcacttctgg gtctcacatt cttggatccg
     1801 gttccaggcc tcggccctaa atagtctccc tgggctttca agagaaccac atgagaaagg
     1861 aggattcggg ctctgagcag tttcaccacc caccccccag tctgcaaatc ctgacccgtg
     1921 ggtccacctg ccccaaaggc ggacgcagga cagtagaagg gaacagagaa cacataaaca
     1981 cagagagggc cacagcggct cccacagtca ccgccacctt cctggcgggg atgggtgggg
     2041 cgtctgagtt tggttcccag caaatccctc tgagccgccc ttgcgggctc gcctcaggag
     2101 caggggagca agaggtggga ggaggaggtc taagtcccag gcccaattaa gagatcaggt
     2161 agtgtagggt ttgggagctt ttaaggtgaa gaggcccggg ctgatcccac aggccagtat
     2221 aaagcgccgt gaccctcagg tgatgcgcca gggccggctg ccgtcgggga cagggctttc
     2281 catagcggcc gcgccgccac catgtggccc ttggcggcgg cgctgttgct gggctcctgc
     2341 tgctgcggtt cagctcaact actgtttagt aacgtcaact ccatagagtt cacttcatgc
     2401 aatgaaactg tggtcatccc ttgcatcgtc cgtaatgtgg aggcgcaaag caccgaagaa
     2461 atgtttgtga agtggaagtt gaacaaatcg tatattttca tctatgatgg aaataaaaat
     2521 agcactacta cagatcaaaa ctttaccagt gcaaaaatct cagtctcaga cttaatcaat
     2581 ggcattgcct ctttgaaaat ggataagcgc gatgccatgg tgggaaacta cacttgcgaa
     2641 gtgacagagt tatccagaga aggcaaaaca gttatagagc tgaaaaaccg cacggccttc
     2701 aacactgacc aaggatcagc ctgttcttac gaggaggaga aaggaggttg caaattagtt
     2761 tcgtggtttt ctccaaatga aaagatcctc attgttattt tcccaatttt ggctatactc
     2821 ctgttctggg gaaagtttgg tattttaaca ctcaaatata aatccagcca tacgaataag
     2881 agaatcattc tgctgctcgt tgccgggctg gtgctcacag tcatcgtggt tgttggagcc
     2941 atccttctca tcccaggaga aaagcccgtg aagaatgctt ctggacttgg cctcattgta
     3001 atctctacgg ggatattaat actacttcag tacaatgtgt ttatgacagc ttttggaatg
     3061 acctctttca ccattgccat attgatcact caagtgctgg gctacgtcct tgctttggtc
     3121 gggctgtgtc tctgcatcat ggcatgtgag ccagtgcacg gccccctttt gatttcaggt
     3181 ttggggatca tagctctagc agaactactt ggattagttt atatgaagtt tgtcgcttcc
     3241 aaccagagga ctatccaacc tcctaggaat aggtgaaccg gtaatcaacc tctggattac
     3301 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3361 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3421 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3481 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3541 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3601 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3661 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     3721 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3781 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgagatct gcctcgactg
     3841 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3901 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3961 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4021 aagacaatag caggcatgct ggggactcga gttaagggcg aattcccgat aaggatcttc
     4081 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     4141 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4201 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4261 agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4321 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4381 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4861 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     4921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6841 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6901 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6961 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7021 aatttcacac aggaaacagc tatgaccatg attacgccag attt
//
