LOCUS       sequence-332023-        5064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 557, Emission: 585.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5064)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mDsRed-H2B-6
FEATURES             Location/Qualifiers
     source          1..5064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             71..865
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             71..865
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(125..144)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     735..754
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1053..1072)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1097..1144
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1473..2061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1962..1981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2236..2539
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2540..2743
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2694..2714
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2832..3209
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3222..3231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3228..3905
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     3626..3645
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4027..4148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4064..4083)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4118..4137
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4155..4610)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4292..4313)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4504..4523
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4637..4741
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(4743..5064,1..36)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4743..4934
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4766..4786)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(4951..5064,1..22)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
       61 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
      121 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
      181 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
      241 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
      301 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
      361 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
      421 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
      481 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
      541 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
      601 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
      661 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
      721 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
      781 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
      841 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
      901 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
      961 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     1021 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     1081 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     1141 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     1201 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     1261 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     1321 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1381 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1441 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1501 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1561 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1621 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1681 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1741 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1801 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1861 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1921 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1981 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2041 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2101 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2161 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
     2221 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     2281 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2341 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2401 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2461 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2521 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2581 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2641 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2701 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
     2761 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg
     2821 gtaccgccac catgccagag ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga
     2881 aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga
     2941 gctattccat ctatgtgtac aaggttctga agcaggtcca ccctgacacc ggcatttcgt
     3001 ccaaggccat gggcatcatg aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg
     3061 aggcttcccg cctggcgcat tacaacaagc gctcgaccat cacctccagg gagatccaga
     3121 cggccgtgcg cctgctgctg cctggggagt tggccaagca cgccgtgtcc gagggtacta
     3181 aggccatcac caagtacacc agcgctaagg atccaccggt cgccaccatg gacaacaccg
     3241 aggacgtcat caaggagttc atgcagttca aggtgcgcat ggagggctcc gtgaacggcc
     3301 actacttcga gatcgagggc gagggcgagg gcaagcccta cgagggcacc cagaccgcca
     3361 agctgcaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
     3421 tccagtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacatgaagc
     3481 tgtccttccc cgagggcttc acctgggagc gctccatgaa cttcgaggac ggcggcgtgg
     3541 tggaggtgca gcaggactcc tccctgcagg acggcacctt catctacaag gtgaagttca
     3601 agggcgtgaa cttccccgcc gacggccccg taatgcagaa gaagactgcc ggctgggagc
     3661 cctccaccga gaagctgtac ccccaggacg gcgtgctgaa gggcgagatc tcccacgccc
     3721 tgaagctgaa ggacggcggc cactacacct gcgacttcaa gaccgtgtac aaggccaaga
     3781 agcccgtgca gctgcccggc aaccactacg tggactccaa gctggacatc accaaccaca
     3841 acgaggacta caccgtggtg gagcagtacg agcacgccga ggcccgccac tccggctccc
     3901 agtaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
     3961 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     4021 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4141 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     4201 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     4261 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4321 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     4381 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4441 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4501 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4561 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     4621 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4681 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4741 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4801 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4861 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4921 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4981 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5041 gcctctgagc tattccagaa gtag
//
