LOCUS       sequence-332030-       19665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Helper/packaging plasmid for production of AAV2/11 pseudotype 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19665)
  AUTHORS   Mori S, Wang L, Takeuchi T, Kanda T
  TITLE     Two novel adeno-associated viruses from cynomolgus monkey: 
            pseudotyping characterization of capsid protein.
  JOURNAL   Virology. 2004 Dec 20;330(2):375-83. doi: 
            10.1016/j.virol.2004.10.012.
  PUBMED    15567432
REFERENCE   2  (bases 1 to 19665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.virol.2004.10"; journalName: "Virology"; date: 
            "2004-12-20- 20"; volume: "330"; issue: "2"; pages: "375-83"
COMMENT     Sequence Label: pEEV2/11
FEATURES             Location/Qualifiers
     source          1..19665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        29..47
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     29..46
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      7188..7197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    12889..13010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12926..12945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12980..12999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     RBS             14295..14303
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(17347..17366)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(17348..17366)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(17453..17470)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17624..18212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17704..17723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(18383..19174,19175..19243))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     19006..19025
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(19244..19348)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     19416..19434
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(19472..19494)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     19594..19613
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acataacctt atgtatcata cacatacgat ttaggtgaca ctatagaact cgagcagagc
       61 ggccgccacg tgtgcgcgcg cgggactaat cccggtccgc gcgtcgggct caaagtcctc
      121 ctcgcgcagc aaccgctcgc ggttcaggcc atgccgcaac tcgcgccctg cgtggaactt
      181 tcgatcccgc atctcctcgg gctcctctcc ctcgcggtcg cgaaacaggt tctgccgcgg
      241 cacgtacgcc tcgcgcgtgt cacgcttcag ctgcaccctt gggtgtcgct caggagaggg
      301 cgctcctagc cgcgccaggc cctcgccctc ctccaagtcc aggtagtgcc gggcccggcg
      361 ccgcgggggt tcgtaatcac catctgccgc cgcgtcagcc gcggatgttg cccctcctga
      421 cgcggtagga gaaggggagg gtgccctgca tgtctgccgc tgctcttgct cttgccgctg
      481 ctgaggaggg gggcgcatct gccgcagcac cggatgcatc tgggaaaagc aaaaaagggg
      541 ctcgtccctg tttccggagg aatttgcaag cggggtcttg catgacgggg aggcaaaccc
      601 ccgttcgccg cagtccggcc ggcccgagac tcgaaccggg ggtcctgcga ctcaaccctt
      661 ggaaaataac cctccggcta cagggagcga gccacttaat gctttcgctt tccagcctaa
      721 ccgcttacgc cgcgcgcggc cagtggccaa aaaagctagc gcagcagccg ccgcgcctgg
      781 aaggaagcca aaaggagcgc tcccccgttg tctgacgtcg cacacctggg ttcgacacgc
      841 gggcggtaac cgcatggatc acggcggacg gccggatccg gggttcgaac cccggtcgtc
      901 cgccatgata cccttgcgaa tttatccacc agaccacgga agagtgcccg cttacaggct
      961 ctccttttgc acggtctaga gcgtcaacga ctgcgcacgc ctcaccggcc agagcgtccc
     1021 gaccatggag cactttttgc cgctgcgcaa catctggaac cgcgtccgcg actttccgcg
     1081 cgcctccacc accgccgccg gcatcacctg gatgtccagg tacatctacg gatatcatcg
     1141 ccttatgttg gaagacctcg cccccggagc cccggccacc ctacgctggc ccctctaccg
     1201 ccagccgccg ccgcactttt tggtgggata tcagtacctg gtgcggactt gcaacgacta
     1261 cgtctttgac tcaagggctt actcgcgtct caggtacacc gagctctcgc agccgggtca
     1321 ccagaccgtt aactggtccg ttatggccaa ctgcacttac accatcaaca cgggcgcata
     1381 ccaccgcttt gtggacatgg atgacttcca gtctaccctc acgcaggtgc agcaggccat
     1441 attagccgag cgcgttgtcg ccgacctggc cctgcttcag ccgatgaggg gcttcggggt
     1501 cacacgcatg ggaggaagag ggcgccacct acggccaaac tccgccgccg ccgtagcgat
     1561 agatgcaaga gatgcaggac aagaggaagg agaagaagaa gtgccggtag aaaggctcat
     1621 gcaagactac tacaaagacc tgcgccgatg tcaaaacgaa gcctggggca tggccgaccg
     1681 cctgcgcatt cagcaggccg gacccaagga catggtgctt ctgtcgactc tagaggatcg
     1741 taccgagaaa tctcgacgag ttgtgggttt tttggctttc gtttctgggc gtaggttcgc
     1801 gtgcggtttt ctgggtgttt tttgtggact ttaaccgtta cgtcattttt tagtcctata
     1861 tatactcgct ctgtacttgg ccctttttac actgtgactg attgagctgg tgccgtgtcg
     1921 agtggtgttt tttaataggt ttttttactg gtaaggctga ctgttatggc tgccgctgtg
     1981 gaagcgctgt atgttgttct ggagcgggag ggtgctattt tgcctaggca ggagggtttt
     2041 tcaggtgttt atgtgttttt ctctcctatt aattttgtta tacctcctat gggggctgta
     2101 atgttgtctc tacgcctgcg ggtatgtatt cccccgggct atttcggtcg ctttttagca
     2161 ctgaccgatg ttaaccaacc tgatgtgttt accgagtctt acattatgac tccggacatg
     2221 accgaggaac tgtcggtggt gctttttaat cacggtgacc agttttttta cggtcacgcc
     2281 ggcatggccg tagtccgtct tatgcttata agggttgttt ttcctgttgt aagacaggct
     2341 tctaatgttt aaatgttttt ttgttatttt attttgtgtt tatgcagaaa cccgcagaca
     2401 tgtttgagag aaaaatggtg tctttttctg tggtggttcc ggagcttacc tgcctttatc
     2461 tgcatgagca tgactacgat gtgctttctt ttttgcgcga ggctttgcct gattttttga
     2521 gcagcacctt gcattttata tcgccgccca tgcaacaagc ttacatcggg gctacgctgg
     2581 ttagcatagc tccgagtatg cgtgtcataa tcagtgtggg ttcttttgtc atggttcctg
     2641 gcggggaagt ggccgcgctg gtccgtgcag acctgcacga ttatgttcag ctggccctgc
     2701 gaagggacct acgggatcgc ggtatttttg ttaatgttcc gcttttgaat cttatacagg
     2761 tctgtgagga acctgaattt ttgcaatcat gattcgctgc ttgaggctga aggtggaggg
     2821 cgctctggag cagattttta caatggccgg acttaatatt cgggatttgc ttagagatat
     2881 attgagaagg tggcgagatg agaattattt gggcatggtt gaaggtgctg gaatgtttat
     2941 agaggagatt caccctgaag ggtttagcct ttacgtccac ttggacgtga gggccgtttg
     3001 ccttttggaa gccattgtgc aacatcttac aaatgccatt atctgttctt tggctgtaga
     3061 gtttgaccac gccaccggag gggagcgcgt tcacttaata gatcttcatt ttgaggtttt
     3121 ggataatctt ttggaataaa aaaaaaaaca tggttcttcc agctcttccc gctcctcccg
     3181 tgtgtgactc gcagaacgaa tgtgtaggtt ggctgggtgt ggcttattct gcggtggtgg
     3241 atgttatcag ggcagcggcg catgaaggag tttacataga acccgaagcc agggggcgcc
     3301 tggatgcttt gagagagtgg atatactaca actactacac agagcgatct aagcggcgag
     3361 accggagacg cagatctgtt tgtcacgccc gcacctggtt ttgcttcagg aaatatgact
     3421 acgtccggcg ttccatttgg catgacacta cgaccaacac gatctcggtt gtctcggcgc
     3481 actccgtaca gtagggatcg tctacctcct tttgagacag aaacccgcgc taccatactg
     3541 gaggatcatc cgctgctgcc cgaatgtaac actttgacaa tgcacaacgt gagttacgtg
     3601 cgaggtcttc cctgcagtgt gggatttacg ctgattcagg aatgggttgt tccctgggat
     3661 atggttctaa cgcgggagga gcttgtaatc ctgaggaagt gtatgcacgt gtgcctgtgt
     3721 tgtgccaaca ttgatatcat gacgagcatg atgatccatg gttacgagtc ctgggctctc
     3781 cactgtcatt gttccagtcc cggttccctg cagtgtatag ccggcgggca ggttttggcc
     3841 agctggttta ggatggtggt ggatggcgcc atgtttaatc agaggtttat atggtaccgg
     3901 gaggtggtga attacaacat gccaaaagag gtaatgttta tgtccagcgt gtttatgagg
     3961 ggtcgccact taatctacct gcgcttgtgg tatgatggcc acgtgggttc tgtggtcccc
     4021 gccatgagct ttggatacag cgccttgcac tgtgggattt tgaacaatat tgtggtgctg
     4081 tgctgcagtt actgtgctga tttaagtgag atcagggtgc gctgctgtgc ccggaggaca
     4141 aggcgcctta tgctgcgggc ggtgcgaatc atcgctgagg agaccactgc catgttgtat
     4201 tcctgcagga cggagcggcg gcggcagcag tttattcgcg cgctgctgca gcaccaccgc
     4261 cctatcctga tgcacgatta tgactctacc cccatgtagg cgtggacttc tccttcgccg
     4321 cccgttaagc aaccgcaagt tggacagcag cctgtggctc agcagctgga cagcgacatg
     4381 aacttaagtg agctgcccgg ggagtttatt aatatcactg atgagcgttt ggctcgacag
     4441 gaaaccgtgt ggaatataac acctaagaat atgtctgtta cccatgatat gatgcttttt
     4501 aaggccagcc ggggagaaag gactgtgtac tctgtgtgtt gggagggagg tggcaggttg
     4561 aatactaggg ttctgtgagt ttgattaagg tacggtgatc tgtataagct atgtggtggt
     4621 ggggctatac tactgaatga aaaatgactt gaaattttct gcaattgaaa aataaacacg
     4681 ttgaaacata acacaaacga ttctttattc ttgggcaatg tatgaaaaag tgtaagagga
     4741 tgtggcaaat atttcattaa tgtagttgtg gccagaccag tcccatgaaa atgacataga
     4801 gtatgcactt ggagttgtgt ctcctgtttc ctgtgtaccg tttagtgtaa tggttagtgt
     4861 tacaggttta gttttgtctc cgtttaagta aacttgactg acaatgttac ttttggcagt
     4921 tttaccgtga gattttggat aagctgatag gttaggcata aatccaacag cgtttgtata
     4981 ggctgtgcct tcagtaagat ctccatttct aaagttccaa tattctgggt ccaggaagga
     5041 attgtttagt agcactccat tttcgtcaaa tcttataata agatgagcac tttgaactgt
     5101 tccagatatt ggagccaaac tgcctttaac agccaaaact gaaactgtag caagtatttg
     5161 actgccacat tttgttaaga ccaaagtgag tttagcatct ttctctgcat ttagtctaca
     5221 gttaggagat ggagctggtg tggtccacaa agttagctta tcattatttt tgtttcctac
     5281 tgtaatggca cctgtgctgt caaaactaag gccagttcct agtttaggaa ccatagcctt
     5341 gtttgaatca aattctaggc catggccaat ttttgttttg aggggatttg tgtttggtgc
     5401 attaggtgaa ccaaattcaa gcccatctcc tgcattaatg gctatggctg tagcgtcaaa
     5461 catcaacccc ttggcagtgc ttaggttaac ctcaagcttt ttggaattgt ttgaagctgt
     5521 aaacaagtaa aggcctttgt tgtagttaat atccaagttg tgggctgagt ttataaaaag
     5581 agggccctgt cctagtctta gatttagttg gttttgagca tcaaacggat aactaacatc
     5641 aagtataagg cgtctgtttt gagaatcaat ccttagtcct cctgctacat taagttgcat
     5701 attgccttgt gaatcaaaac ccaaggctcc agtaacttta gtttgcaagg aagtattatt
     5761 aatagtcaca cctggaccag ttgctacggt caaagtgttt aggtcgtctg ttacatgcaa
     5821 aggagccccg tactttagtc ctagttttcc attttgtgta taaatgggct ctttcaagtc
     5881 aatgcccaag ctaccagtgg cagtagttag agggggtgag gcagtgatag taagggtact
     5941 gctatcggtg gtggtgaggg ggcctgatgt ttgcagggct agctttcctt ctgacactgt
     6001 gaggggtcct tgggtggcaa tgctaagttt ggagtcgtgc acggttagcg gggcctgtga
     6061 ttgcatggtg agtgtgttgc ccgcgaccat tagaggtgcg gcggcagcca cagttagggc
     6121 ttctgaggta actgtgaggg gtgcagatat ttccaggttt atgtttgact tggttttttt
     6181 gagaggtggg ctcacagtgg ttacattttg ggaggtaagg ttgccggcct cgtccagaga
     6241 gaggccgttg cccattttga gcgcaagcat gccattggag gtaactagag gttcggatag
     6301 gcgcaaagag agtaccccag ggggactctc ttgaaaccca ttgggggata caaagggagg
     6361 agtaagaaaa ggcacagttg gaggaccggt ttccgtgtca tatggataca cggggttgaa
     6421 ggtatcttca gacggtcttg cgcgcttcat ctgcaacaac atgaagatag tgggtgcgga
     6481 tggacaggaa caggaggaaa ctgacattcc atttagattg tggagaaagt ttgcagccag
     6541 gaggaagctg caataccaga gctgggagga gggcaaggag gtgctgctga ataaactgga
     6601 cagaaatttg ctaactgatt ttaagtaagt gatgctttat tatttttttt tattagttaa
     6661 agggaataag atctttgaga ccgcacaggg tcttaataag ggtgcagaga tcctcaggtc
     6721 cttgacaagg tgagtgaatg cagccttcgg tttctaccga gtgctgagtt atggtaatgg
     6781 gcttttctcc caccatgacc accaatttct gacgcttggt tggcaacttg tagctaaggc
     6841 ggtgtccggt ggtattactg tcgtaggtga ctttggcctg ctttaccaga caaaagatac
     6901 cccttttgca ctggtgcaag ttaaccatgt cttggagctc ttgattcatg cgctgttgct
     6961 cggccgctgc cctgcgtctt tctagcaggc gctgctctgt aataattccg tccatttcta
     7021 gaggatccca tggacgagcc cacccttctt tatgttttgt ttgaagtctt tgacgtggtc
     7081 cgtgtgcacc agccgcaccg cggcgtcatc gaaaccgtgt acctgcgcac gcccttctcg
     7141 gccggcaacg ccacaacata aagaagcaag caacatcaac aacagctgcc gccatgggct
     7201 ccagtgagca ggaactgaaa gccattgtca aagatcttgg ttgtgggcca tattttttgg
     7261 gcacctatga caagcgcttt ccaggctttg tttctccaca caagctcgcc tgcgccatag
     7321 tcaatacggc cggtcgcgag actgggggcg tacactggat ggcctttgcc tggaacccgc
     7381 actcaaaaac atgctacctc tttgagccct ttggcttttc tgaccagcga ctcaagcagg
     7441 tttaccagtt tgagtacgag tcactcctgc gccgtagcgc cattgcttct tcccccgacc
     7501 gctgtataac gctggaaaag tccacccaaa gcgtacaggg gcccaactcg gccgcctgtg
     7561 gactattctg ctgcatgttt ctccacgcct ttgccaactg gccccaaact cccatggatc
     7621 acaaccccac catgaacctt attaccgggg tacccaactc catgctcaac agtccccagg
     7681 tacagcccac cctgcgtcgc aaccaggaac agctctacag cttcctggag cgccactcgc
     7741 cctacttccg cagccacagt gcgcagatta ggagcgccac ttctttttgt cacttgaaaa
     7801 acatgtaaaa ataatgtact agagacactt tcaataaagg caaatgcttt tatttgtaca
     7861 ctctcgggtg attatttacc cccacccttg ccgtctgcgc cgtttaaaaa tcaaaggggt
     7921 tctgccgcgc atcgctatgc gccactggca gggacacgtt gcgatactgg tgtttagtgc
     7981 tccacttaaa ctcaggcaca accatccgcg gcagctcggt gaagttttca ctccacaggc
     8041 tgcgcaccat caccaacgcg tttagcaggt cgggcgccga tatcttgaag tcgcagttgg
     8101 ggcctccgcc ctgcgcgcgc gagttgcgat acacagggtt gcagcactgg aacactatca
     8161 gcgccgggtg gtgcacgctg gccagcacgc tcttgtcgga gatcagatcc gcgtccaggt
     8221 cctccgcgtt gctcagggcg aacggagtca actttggtag ctgccttccc aaaaagggcg
     8281 cgtgcccagg ctttgagttg cactcgcacc gtagtggcat caaaaggtga ccgtgcccgg
     8341 tctgggcgtt aggatacagc gcctgcataa aagccttgat ctgcttaaaa gccacctgag
     8401 cctttgcgcc ttcagagaag aacatgccgc aagacttgcc ggaaaactga ttggccggac
     8461 aggccgcgtc gtgcacgcag caccttgcgt cggtgttgga gatctgcacc acatttcggc
     8521 cccaccggtt cttcacgatc ttggccttgc tagactgctc cttcagcgcg cgctgcccgt
     8581 tttcgctcgt cacatccatt tcaatcacgt gctccttatt tatcataatg cttccgtgta
     8641 gacacttaag ctcgccttcg atctcagcgc agcggtgcag ccacaacgcg cagcccgtgg
     8701 gctcgtgatg cttgtaggtc acctctgcaa acgactgcag gtacgcctgc aggaatcgcc
     8761 ccatcatcgt cacaaaggtc ttgttgctgg tgaaggtcag ctgcaacccg cggtgctcct
     8821 cgttcagcca ggtcttgcat acggccgcca gagcttccac ttggtcaggc agtagtttga
     8881 agttcgcctt tagatcgtta tccacgtggt acttgtccat cagcgcgcgc gcagcctcca
     8941 tgcccttctc ccacgcagac acgatcggca cactcagcgg gttcatcacc gtaatttcac
     9001 tttccgcttc gctgggctct tcctcttcct cttgcgtccg cataccacgc gccactgggt
     9061 cgtcttcatt cagccgccgc actgtgcgct tacctccttt gccatgcttg attagcaccg
     9121 gtgggttgct gaaacccacc atttgtagcg ccacatcttc tctttcttcc tcgctgtcca
     9181 cgattacctc tggtgatggc gggcgctcgg gcttgggaga agggcgcttc tttttcttct
     9241 tgggcgcaat ggccaaatcc gccgccgagg tcgatggccg cgggctgggt gtgcgcggca
     9301 ccagcgcgtc ttgtgatgag tcttcctcgt cctcggactc gatacgccgc ctcatccgct
     9361 tttttggggg cgcccgggga ggcggcggcg acggggacgg ggacgacacg tcctccatgg
     9421 ttgggggacg tcgcgccgca ccgcgtccgc gctcgggggt ggtttcgcgc tgctcctctt
     9481 cccgactggc catttccttc tcctataggc agaaaaagat catggagtca gtcgagaaga
     9541 aggacagcct aaccgccccc tctgagttcg ccaccaccgc ctccaccgat gccgccaacg
     9601 cgcctaccac cttccccgtc gaggcacccc cgcttgagga ggaggaagtg attatcgagc
     9661 aggacccagg ttttgtaagc gaagacgacg aggaccgctc agtaccaaca gaggataaaa
     9721 agcaagacca ggacaacgca gaggcaaacg aggaacaagt cgggcggggg gacgaaaggc
     9781 atggcgacta cctagatgtg ggagacgacg tgctgttgaa gcatctgcag cgccagtgcg
     9841 ccattatctg cgacgcgttg caagagcgca gcgatgtgcc cctcgccata gcggatgtca
     9901 gccttgccta cgaacgccac ctattctcac cgcgcgtacc ccccaaacgc caagaaaacg
     9961 gcacatgcga gcccaacccg cgcctcaact tctaccccgt atttgccgtg ccagaggtgc
    10021 ttgccaccta tcacatcttt ttccaaaact gcaagatacc cctatcctgc cgtgccaacc
    10081 gcagccgagc ggacaagcag ctggccttgc ggcagggcgc tgtcatacct gatatcgcct
    10141 cgctcaacga agtgccaaaa atctttgagg gtcttggacg cgacgagaag cgcgcggcaa
    10201 acgctctgca acaggaaaac agcgaaaatg aaagtcactc tggagtgttg gtggaactcg
    10261 agggtgacaa cgcgcgccta gccgtactaa aacgcagcat cgaggtcacc cactttgcct
    10321 acccggcact taacctaccc cccaaggtca tgagcacagt catgagtgag ctgatcgtgc
    10381 gccgtgcgca gcccctggag agggatgcaa atttgcaaga acaaacagag gagggcctac
    10441 ccgcagttgg cgacgagcag ctagcgcgct ggcttcaaac gcgcgagcct gccgacttgg
    10501 aggagcgacg caaactaatg atggccgcag tgctcgttac cgtggagctt gagtgcatgc
    10561 agcggttctt tgctgacccg gagatgcagc gcaagctaga ggaaacattg cactacacct
    10621 ttcgacaggg ctacgtacgc caggcctgca agatctccaa cgtggagctc tgcaacctgg
    10681 tctcctacct tggaattttg cacgaaaacc gccttgggca aaacgtgctt cattccacgc
    10741 tcaagggcga ggcgcgccgc gactacgtcc gcgactgcgt ttacttattt ctatgctaca
    10801 cctggcagac ggccatgggc gtttggcagc agtgcttgga ggagtgcaac ctcaaggagc
    10861 tgcagaaact gctaaagcaa aacttgaagg acctatggac ggccttcaac gagcgctccg
    10921 tggccgcgca cctggcggac atcattttcc ccgaacgcct gcttaaaacc ctgcaacagg
    10981 gtctgccaga cttcaccagt caaagcatgt tgcagaactt taggaacttt atcctagagc
    11041 gctcaggaat cttgcccgcc acctgctgtg cacttcctag cgactttgtg cccattaagt
    11101 accgcgaatg ccctccgccg ctttggggcc actgctacct tctgcagcta gccaactacc
    11161 ttgcctacca ctctgacata atggaagacg tgagcggtga cggtctactg gagtgtcact
    11221 gtcgctgcaa cctatgcacc ccgcaccgct ccctggtttg caattcgcag ctgcttaacg
    11281 aaagtcaaat tatcggtacc tttgagctgc agggtccctc gcctgacgaa aagtccgcgg
    11341 ctccggggtt gaaactcact ccggggctgt ggacgtcggc ttaccttcgc aaatttgtac
    11401 ctgaggacta ccacgcccac gagattaggt tctacgaaga ccaatcccgc ccgcctaatg
    11461 cggagcttac cgcctgcgtc attacccagg gccacattct tggccaattg caagccatca
    11521 acaaagcccg ccaagagttt ctgctacgaa agggacgggg ggtttacttg gacccccagt
    11581 ccggcgagga gctcaaccca atccccccgc cgccgcagcc ctatcagcag cagccgcggg
    11641 cccttgcttc ccaggatggc acccaaaaag aagctgcagc tgccgccgcc acccacggac
    11701 gaggaggaat actgggacag tcaggcagag gaggttttgg acgaggagga ggaggacatg
    11761 atggaagact gggagagcct agacgaggaa gcttccgagg tcgaagaggt gtcagacgaa
    11821 acaccgtcac cctcggtcgc attcccctcg ccggcgcccc agaaatcggc aaccggttcc
    11881 agcatggcta caacctccgc tcctcaggcg ccgccggcac tgcccgttcg ccgacccaac
    11941 cgtagatggg acaccactgg aaccagggcc ggtaagtcca agcagccgcc gccgttagcc
    12001 caagagcaac aacagcgcca aggctaccgc tcatggcgcg ggcacaagaa cgccatagtt
    12061 gcttgcttgc aagactgtgg gggcaacatc tccttcgccc gccgctttct tctctaccat
    12121 cacggcgtgg ccttcccccg taacatcctg cattactacc gtcatctcta cagcccatac
    12181 tgcaccggcg gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag
    12241 caagactctg acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct
    12301 gcgtctggcg cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac
    12361 tctgtatgct atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag
    12421 gtctctgcga tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg
    12481 cacgctggaa gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta
    12541 gtttcgcgcc ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc
    12601 ggcgccagca cctgttgtca gcgccattat gagcaaggaa attcccacgc cctacatgtg
    12661 gagttaccag ccacaaatgg gacttgcggc tggagctgcc caagactact caacccgaat
    12721 aaactacatg agcgcgggac cccacatgat atcccgggtc aacggaatac gcgcccaccg
    12781 aaaccgaatt catcgcctac cacatttgta gaggttttac ttgctttaaa aaacctccca
    12841 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    12901 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    12961 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctat
    13021 cgatgcctgc aggttacaaa tgattagtca aataacgaga gccaataacc cgcggctctg
    13081 tatacttccc agttgtatca ggagcccaca acatagaaga ctggttccca tagtttgaag
    13141 taaactgcac ttcaggattc cagcgtttgg agcgttcctt ttcaatttcc cattcaatct
    13201 gaacagcgac ctggccggtg ctgtattgtg tgatgaaaga gtccactctg gctgcagtga
    13261 aggttgtcgc aggattggca ggtacgggag tgttcttgat gaatatctgg ggaggcgggt
    13321 gtttcagtcc aaacccacca atcagcggtg aaggatgaaa atgtccgtcc gcgtgtggga
    13381 tcttggccca aattggccct tggtagtaaa tgtctctgtt ttgccacacc atgccaggca
    13441 gcactcccat agcagtcacg ttgccggtta tgggagcagt tgtagcattc tgattattgt
    13501 cagcaatctg gccaaacatg tccgtgtctc ttgggttggt ggcagcaatt tcttcttctg
    13561 atgtaaacaa cagattgttg gctgaagttg ttgtatttcc ggtaacagat ggtccaggga
    13621 atataagctg ggcgttactg aagtccccat ccgaaggtcc ggctgtggcc attggaggtc
    13681 cgggcgcgat gttgctccag cggttgttta aggtatagtg ggtgtcatac tttaacagag
    13741 cgttgccccc gctggcagga atcttgtaat tttgactggc agtttttgag aatctctgct
    13801 gtttaacaca aggcccaggc agccagttct ttctgtaaaa ggcaaagtct ccactcctga
    13861 tttttccaaa tgtggttgct gcattgcctt gattcagagt ctctccagag gtagtcgact
    13921 gtaagtgcca caggtactgg tccaggaggg gattcatcag tctgtccagg ctctggctgt
    13981 gagcatacat tgagtggaac ggcaccttct caaagttgta agccatttca aagttgttgc
    14041 cagttctcaa catttgcgaa ggaaaatact ccaggcagta gaaagcgttt ctgtccgttt
    14101 ggttctgatt ctcgccagtc acgatgccac agtagccata ttgaggcacc atgaacacgt
    14161 cattggggaa aggaggcagg ctcccctctt gtccagcgtc catcacgtac gggagctcat
    14221 acgacgagtc cgcaaatatc tgaaccgtgc tggtaaggtt attagcgacc gtagtctcgc
    14281 cgttcgacgt tgtgacctcc ttaacttgga tattgaagat tttaacgcgc atggcttttg
    14341 gtcgtagtcc ccagttgttg ttgatgagtc tttgccagtc acgtggtgag aagtgacagt
    14401 ggaatctgtt gaagtcaaaa tatccccagg gggtggagaa tccgttgtag gtgttgctgc
    14461 ttgatgttgt tccgagacgc aggtacaagt ggttgttgta ggtgggcaag acccaggttc
    14521 tggtcgaggt tgttgtgacc ttgccctcag accaggtgga atcgcaatgc caatcacccg
    14581 aggcattacc cactccatcg gaaccttgtc ccgcatcgac agcatttccg cccggtgctg
    14641 cacgcatttc aatgtctgaa gacatggcgc tggtatctga tccttcaggg ggtccgtctc
    14701 cggctccagt gtcctcttca aagttgagcc tctttctggc tggttgtttg ccttttttgc
    14761 cgatgcccga ggaggagtcg ggctcttgtg gtgactctaa cggtctcttc tttccaggag
    14821 ccgttttagc accttcttca accaggccca gaggttcgag taccctcttc ttggcctgga
    14881 agactgctcg cccgaggttg cccccaaaag acgtatcttc ttgcagacgc tcctgaaact
    14941 cggcgtcggc gtggttatac cgcaggtacg gattgtcacc cgctttgagc tgctggtcgt
    15001 aggccttgtc gtgctcgagg gccgctgcgt ccgccgcgtt gacgggctcc cccttgtcga
    15061 gtccgttgaa gggtccgagg tacttgtagc caggaagcac cagaccccgg ccgtcgtcct
    15121 gcttctgctg gttggccttg ggcttcgggg ctccaggttt caggtcccac cactcgcgaa
    15181 tgccctcaga gaggttgtcc tcgagccaat ctggaagata accgtcagca gccatacctg
    15241 gtttaagtca tttattgctc agaaacacag tcatccaagt ccacgttgac gagatcgcag
    15301 gccgaacacg caatctcggg tgcccgcccc agcagatgat gaatcgcgca cagtttctga
    15361 tacgtctttt ttctgacgac gggttgagat tctgacgcgc cggggaagca ctctgagcag
    15421 tctctgaccc cgtgcgtgaa gcagacgttg aaattctgat tcattctctc gcatgtcttg
    15481 cagggaaaca gcatctgaag catgcccgcg tgacgagaac atttgttttg gtacctgtcc
    15541 gcaaagtcca ccggtgcttc cgcgtctgac gtcgatggct ccggaactga ggggcaggcc
    15601 cgcttgggct cgcttatatc cgcgtcactg ggggcgggtc ttttggtggc tccgcccttt
    15661 ctgacgtaga actcatgcgc cacctcagtc acgtgatcct gagcccagcg gaagaactct
    15721 ttgacttcct gcttggtcac cttgccaaag tcgtgctcca gacggcgggt gagctcgaac
    15781 ttgaacatgc ggtcctgcag cggctgctgg tgctcgaagg tggtgctgtt cccgtcgatc
    15841 acggcgcaca tgttggtgtt ggaggtgacg atcacgggcg tggggtcgat ctgggccgag
    15901 gacttgcact tttggtccac gcgcaccttg cttccgccca gaatggcctt ggcggactcc
    15961 acgaccttgg cggtcatctt gccctcctcc caccagatca ccatcttgtc gacacagtcg
    16021 ttgaagggaa agttctcatt ggtccagttt acgcacccgt agaagggcac agtgtgggct
    16081 atggcctccg cgatgttggt cttcccggta gttgcaggcc caaacagcca gatggtgttc
    16141 ctcttgccga actttttcgt ggcccatccc agaaagacgg aagccgcata ttggggatcg
    16201 tacccgttta gttccaaaat tttataaatc cgattgctgg aaatgtcctc cacgggctgc
    16261 tggcccacca ggtagtcggg ggcggtttta gtcaggctca taatctttcc cgcattgtcc
    16321 aaggcagcct tgatttggga ccgcgagttg gaggccgcat tgaaggagat gtatgaggcc
    16381 tggtcctcct ggatccactg cttctccgag gtaatcccct tgtccacgag ccacccgacc
    16441 agctccatgt acctggctga agtttttgat ctgatcaccg gcgcatcaga attgggattc
    16501 tgattctctt tgttctgctc ctgcgtctgc gacacgtgcg tcagatgctg cgccaccaac
    16561 cgtttacgct ccgtgagatt caaacaggcg cttaaatact gttccatatt agtccacgcc
    16621 cactggagct caggctgggt tttggggagc aagtaattgg ggatgtagca ctcatccacc
    16681 accttgttcc cgcctccggc gccatttctg gtctttgtga ccgcgaacca gtttggcaaa
    16741 gtcggctcga tcccgcggta aattctctga atcagttttt cgcgaatctg actcaggaaa
    16801 cgtcccaaaa ccatggattt caccccggtg gtttccacga gcacgtgcat gtggaagtag
    16861 ctctctccct tctcaaattg cacaaagaaa agggcctccg gggccttact cacacggcgc
    16921 cattccgtca gaaagtcgcg ctgcagcttc tcggccacgg tcaggggtgc ctgctcaatc
    16981 agattcagat ccatgtcaga atctggcggc aactcccatt ccttctcggc cacccagttc
    17041 acaaagctgt cagaaatgcc gggcagatgc tcgtcaaggt cgctggggac cttaatcaca
    17101 atctcgtaaa accccggcgt ggcggctgcg cgttcaaacc tcccgcttca aaatggagac
    17161 cctgcgtgct cactcgggct taaataccca gcgtgaccac atggtgtcgc aaaatgtcgc
    17221 aaaacactca cgtgacctct aatacaggac ctccctaacc ctatgacgta attcacgtca
    17281 cgactccacc cctccaggaa ccccctctag aggatccccc atcgatgata tcagatctgc
    17341 cggtctccct atagtgagtc gtattaattt cgataagcca ggttaacctg cattaatgaa
    17401 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    17461 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    17521 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    17581 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    17641 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    17701 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    17761 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    17821 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    17881 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    17941 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    18001 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    18061 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    18121 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    18181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    18241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    18301 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    18361 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    18421 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    18481 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    18541 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    18601 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    18661 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    18721 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    18781 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    18841 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    18901 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    18961 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    19021 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    19081 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    19141 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    19201 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    19261 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    19321 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    19381 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    19441 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    19501 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    19561 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    19621 tgcaccatat ggacatattg tcgttagaac gcggctacaa ttaat
//
