LOCUS       sequence-332032-        9927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140 
            under the EF1A promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Boassa D, Lemieux SP, Lev-Ram V, Hu J, Xiong Q, Phan S, Mackey M, 
            Ramachandra R, Peace RE, Adams SR, Ellisman MH, Ngo JT
  TITLE     Split-miniSOG for Spatially Detecting Intracellular Protein-Protein 
            Interactions by Correlated Light and Electron Microscopy.
  JOURNAL   Cell Chem Biol. 2019 Oct 17;26(10):1407-1416.e5. doi: 
            10.1016/j.chembiol.2019.07.007. Epub 2019 Aug 1.
  PUBMED    31378710
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2019.07.007"; journalName: "Cell Chem Biol"; 
            date: "2019-10-17- 17"; volume: "26"; issue: "10"; pages: 
            "1407-1416.e5"
COMMENT     Sequence Label: pLV EF1A mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140
FEATURES             Location/Qualifiers
     source          1..9927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    237..786
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(381..398)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     608..627
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    1241..1829
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1294..1314)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1712..1723)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2036..2669
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2797..2813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2797..2813)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2810..2832)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2821..2837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2845..2851,2852..2869,2870..2875))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2890..2911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3028..3045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3199..3787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3279..3298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3958..4749,4750..4818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4581..4600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4819..4923)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4971..5105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5008..5027)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5062..5081
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5273..5906
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5953..6078
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6575..6808
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6993..7037
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7300..7415
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        7493..7704
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          7705..8643
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8600..8620
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8691..8700
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9333..9356
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9372..9428
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9456..9509
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9513..9539
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9552..9872
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 gagaactgca gtattttatt ggagtgcagc tcgacgggtc cgatcacgta gaaccactgc
       61 aaaacaggct ctcagagagg acggagatgc agagtagtaa gctcgtgaaa gctaccgcca
      121 ccaacgtcga tgaggccgtc tgactctaga gtcgacgggc cgcggtaaca attgttaact
      181 aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt
      241 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
      301 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
      361 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
      421 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
      481 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
      541 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
      601 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
      661 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
      721 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
      781 ataataccat ggtcatgaaa acatttaaca tttctcaaca agatctagaa ttagtagaag
      841 tagcgacaga gaagattaca atgctttatg aggataataa acatcatgtg ggagcggcaa
      901 ttcgtacgaa aacaggagaa atcatttcgg cagtacatat tgaagcgtat ataggacgag
      961 taactgtttg tgcagaagcc attgcgattg gtagtgcagt ttcgaatgga caaaaggatt
     1021 ttgacacgat tgtagctgtt agacaccctt attctgacga agtagataga agtattcgag
     1081 tggtaagtcc ttgtggtatg tgtagggagt tgatttcaga ctatgcacca gattgttttg
     1141 tgttaataga aatgaatggc aagttagtca aaactacgat tgaagaactc attccactca
     1201 aatatacccg aaattaaccc gggcggggcg cgtctggaac aatcaacctc tggattacaa
     1261 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     1321 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     1381 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     1441 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     1501 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     1561 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     1621 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     1681 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     1741 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     1801 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
     1861 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     1921 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     1981 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa
     2041 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     2101 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     2161 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     2221 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     2281 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     2341 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     2401 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     2461 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     2521 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     2581 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     2641 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     2701 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt
     2761 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     2821 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     2881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2941 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3001 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3061 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3121 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3181 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3241 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3541 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3601 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3661 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3721 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3781 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3841 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4081 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4261 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4561 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4861 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4921 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta
     4981 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5041 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5101 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     5161 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     5221 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     5281 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     5341 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     5401 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     5461 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     5521 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     5581 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     5641 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     5701 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     5761 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5821 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5881 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     5941 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     6001 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     6061 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     6121 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     6181 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     6241 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     6301 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     6361 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     6421 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     6481 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     6541 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     6601 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     6661 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     6721 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     6781 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     6841 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     6901 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     6961 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     7021 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     7081 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     7141 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     7201 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     7261 gatccattcg attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa aggggggatt
     7321 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     7381 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     7441 gatccagttt atcgatgagg ccctttcgtc ttcactcgag gtgcccgtca gtgggcagag
     7501 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     7561 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     7621 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     7681 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     7741 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     7801 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     7861 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     7921 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     7981 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     8041 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     8101 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     8161 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     8221 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     8281 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     8341 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     8401 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     8461 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     8521 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     8581 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     8641 caggtgtcgt gaggatccgc ggccgcgccg gctctagatc gcgaacgcgt gccaccatgg
     8701 agaagagttt tgtcatcact gatcctaggc tccccgataa tcctatcatc ttcgcgtccg
     8761 atggttttct ggagctcacc gagtactccc gcgaagaaat cctgggacgc aacgggcggt
     8821 tcctgcaggg gccagagacc gatcaggcca ccgttcagaa gattcgcgac gctataagag
     8881 atcagcgcga gattactgtc caactcataa attacacaaa atccggaaaa aagttctgga
     8941 acttgctgca tctgcagccc atgcgcgatc agaagggtgg aggcagtagt ggcgggggta
     9001 agactggcga gtctatggcc tctaggatcg tctggcatga gatgtggcac gagggccttg
     9061 aggaggcctc tcgcctctat ttcggcgaga ggaatgtaaa aggcatgttt gaagtcctcg
     9121 aaccccttca tgcaatgatg gaacgcggcc ctcagaccct taaagaaaca tcttttaacc
     9181 aagcgtatgg aagggatctg atggaggccc aagaatggtg ccggaaatac atgaaaagcg
     9241 ggaatgttaa ggaccttttg caagcatggg atctctacta tcacgtgttc agacgcatca
     9301 gtaagtccgg gcggggctcc tacgtcacta gtgattacaa ggatgacgat gacaagggga
     9361 gcggcagcgg cgccacaaac ttctctctgc taaagcaagc aggtgatgtt gaagaaaacc
     9421 ccgggcctac tagcgctgga aagcttggct ccggcgaggg caggggaagt cttctaacat
     9481 gcggggacgt ggaggaaaat cccggcccaa gttatcccta cgatgtgccc gattacgctc
     9541 atatgagcag gggagtacag gtagagacga tatctcccgg tgacggacga acatttccca
     9601 aacgcggaca gacatgtgtc gtgcactaca cagggatgct ggaagatggg aagaaatttg
     9661 atagcagccg ggatcgcaac aagccgttca agttcatgct gggcaagcag gaagttatca
     9721 ggggatggga ggaaggcgtg gcacagatgt ccgtgggaca acgcgcaaag ctgacaatct
     9781 cacccgacta cgcttatggc gcgaccggac atcccggaat tattcctcct cacgccacgc
     9841 tggtttttga tgttgagctg ctgaaactgg aatccgggag agggtcctac gttagtggtc
     9901 ggtctattgc caccggcagt gggagcg
//
