LOCUS       sequence-332045-        8309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of human TNF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Pinci F, Gaidt MM, Jung C, Kuut G, Jackson MA, Bauernfried S, 
            Hornung V
  TITLE     C-tag TNF: a reporter system to study TNF shedding.
  JOURNAL   J Biol Chem. 2020 Dec 25;295(52):18065-18075. doi: 
            10.1074/jbc.RA120.015248. Epub 2020 Oct 20.
  PUBMED    33082141
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA120.015248"; journalName: "J Biol Chem"; date: 
            "2020-12-25- 25"; volume: "295"; issue: "52"; pages: "18065-18075"
COMMENT     Sequence Label: pLI_TNF
FEATURES             Location/Qualifiers
     source          1..8309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        172..486
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    251..269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    287..305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    323..341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    358..376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    394..412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     459..483
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1211..1721
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1640..1658
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1731..2327
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1731..1750)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2227..2247
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2328..2381
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2382..2999,3000..3005,3006..3041,3042..3044,3045..3080,
                     3081..3083,3084..3119,3120..3128)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3168..3188)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3586..3597)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3802..4035
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4107..4228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4144..4163)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4198..4217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4268..4403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4403..4474
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4475..4543,4544..5335)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4693..4712)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5506..6094
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5995..6014
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6248..6265
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6382..6403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6418..6423,6424..6441,6442..6448)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6456..6472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6461..6483
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6480..6496
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6480..6496
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6515..6535
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6517..6535
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6563..6789
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6790..6970
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7017..7142
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7635..7868
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8053..8097
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccact ttggccgcga atcgatatgt cgagtttact
      181 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
      241 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      301 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
      361 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      421 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      481 atcgcctgga gaattggcta gcatgagcac tgaaagcatg atccgggacg tggagctggc
      541 cgaggaggcg ctccccaaga agacaggggg gccccagggc tccaggcggt gcttgttcct
      601 cagcctcttc tccttcctga tcgtggcagg cgccaccacg ctcttctgcc tgctgcactt
      661 tggagtgatc ggcccccaga gggaagagtt ccccagggac ctctctctaa tcagccctct
      721 ggcccaggca gtcagatcat cttctcgaac cccgagtgac aagcctgtag cccatgttgt
      781 agcaaaccct caagctgagg ggcagctcca gtggctgaac cgccgggcca atgccctcct
      841 ggccaatggc gtggagctga gagataacca gctggtggtg ccatcagagg gcctgtacct
      901 catctactcc caggtcctct tcaagggcca aggctgcccc tccacccatg tgctcctcac
      961 ccacaccatc agccgcatcg ccgtctccta ccagaccaag gtcaacctcc tctctgccat
     1021 caagagcccc tgccagaggg agaccccaga gggggctgag gccaagccct ggtatgagcc
     1081 catctatctg ggaggggtct tccagctgga gaagggtgac cgactcagcg ctgagatcaa
     1141 tcggcccgac tatctcgact ttgccgagtc tgggcaggtc tactttggga tcattgccct
     1201 gtgaggatcc ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     1261 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     1321 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     1381 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     1441 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     1501 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     1561 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     1621 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     1681 tcggctccct cgttgaccga atcaccgacc tctctcccca gcaattcacc atgaccgagt
     1741 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     1801 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     1861 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     1921 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     1981 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     2041 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     2101 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     2161 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     2221 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     2281 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgaa ggtagaggtt
     2341 ctctcctcac ttgtggtgat gttgaagaaa accctggtcc aatgtctaga ctggacaaga
     2401 gcaaagtcat aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga
     2461 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
     2521 acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact
     2581 tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc
     2641 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
     2701 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
     2761 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
     2821 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
     2881 ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
     2941 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
     3001 ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
     3061 actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc
     3121 ccgggtaact aagtaaggat cgatccaaga tatcgtattc ttaactatgt tgctcctttt
     3181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3481 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     3541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3661 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtttc gcctcggcgt
     3721 ccggactaga ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     3781 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3841 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3901 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3961 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4021 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     4081 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     4141 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4201 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     4261 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4321 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4381 gtgaggaggc ttttttggag gcctttcaaa tatgtatccg ctcatgagac aataaccctg
     4441 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4501 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4561 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4621 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4681 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4741 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4801 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4861 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4921 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4981 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5041 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5101 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5161 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5221 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5281 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5341 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5401 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5461 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5521 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5581 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5641 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5701 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5761 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5821 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5881 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5941 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6001 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6061 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6121 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6181 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6241 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6301 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6361 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6421 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6481 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     6541 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     6601 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     6661 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     6721 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     6781 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6841 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6901 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6961 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     7021 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     7081 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     7141 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     7201 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     7261 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     7321 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     7381 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     7441 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     7501 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     7561 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     7621 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     7681 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     7741 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     7801 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     7861 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     7921 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     7981 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     8041 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     8101 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     8161 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     8221 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggaca attctcgacc
     8281 tcgagacaaa tggcagtatt catccacaa
//
