LOCUS       sequence-332050-       13583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, 
            ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Fracchiolla D, Chang C, Hurley JH, Martens S
  TITLE     A PI3K-WIPI2 positive feedback loop allosterically activates LC3 
            lipidation in autophagy.
  JOURNAL   J Cell Biol. 2020 Jul 6;219(7). pii: 151802. doi: 
            10.1083/jcb.201912098.
  PUBMED    32437499
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-07-6"; volume: "219(7). pii: 151802. doi"; 
            pages: " 10.1083/jcb.201912098"
COMMENT     Sequence Label: 
            10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human 
            autophagy E3(mGFP)-like enzyme)
FEATURES             Location/Qualifiers
     source          1..13583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    166..300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(444..461)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1346..1366)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(1373..1402)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(1461..1552)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        join(1712..1717,1718..1734,1735..1740)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1929..2462
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  2529..2753
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(2887..2904)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3058..3646)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3138..3157)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3757..3776)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3768..4583
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3768..4583
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3841..3860)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4649..4668
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  4939..5104
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    5264..5398
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5542..5559)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(5613..5636)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(5655..5675)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(join(5688..6398,6399..6401,6402..6404))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5730..5751)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6079..6098
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6338..6359
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(8296..8387)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    8559..8693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8837..8854)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(9383..9474)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    9646..9780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9924..9941)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(10710..10801)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    10973..11107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(11251..11268)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(13486..13577)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
ORIGIN
        1 atattccgga gtaggtcgcg aatcgatact agtgtttaaa ctgttccaat tagatgggta
       61 taccctaggg gttatgatag ttattgctca gcggtggcag cagccaactc agcttccttt
      121 cgggctttgt tagcagccgg atcttctagg ctcaagcagt gatcagatcc agacatgata
      181 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      241 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      301 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      361 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct agtacttctc
      421 gacaagcttg gtaccgcatg tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc
      481 ttatattccc cagaacatca ggttaatggc gttagtccgt aggttgggga ataatggaaa
      541 tgtggaggaa gttgtctggg taggagaggt gttcggacag ccattgcaga ggggtttcca
      601 gcattggctc gattccgtgg atcatgacct ggttcttctt ttctccgtcc tcagggtcga
      661 tagctgatgg gcagacctcc ttcagcaggt cgcccagagt gtgcagctga ccgtcggcag
      721 ccactggtct gaacagcttc tggatgaagg gcctttcggt agtggtctgg tagatacgga
      781 aggggatgta gcggaagccg ttttcctcgg cagggtactc catcagctta cggttgatag
      841 cccagaactg gtcgaagcgg tcgttttgca gacccatcca cagctgcttg tggtccttct
      901 tctgcatttc gttgatgacc tgggacttgt gcttcagggc gtcagcttcc ttcatgcagg
      961 acatgaagtg ggcctcgata gcgtcctttg aagggcagtg cagcaggtcc ttctctggga
     1021 agctcttgaa gtggacggtg atgttccaag gcagggcaga gctggaagcc agcaggtcga
     1081 acagcagacc gatggggtag tgccacttca gaggagttcc ttcgtactcg aaccagattt
     1141 cagagatgtc ctcctgcctc atgaccttct ggaagtgctt cttgaccttg tcggtcacca
     1201 gagtcaggta gctgactctt ggcagcagca ggtagtaggg ttcagcctcc ctctccgtaa
     1261 tctcatcttg gtacagcgtg aagcaggtag gaatgcgtcc gaaccagaca tctctcagaa
     1321 catctttatc atccgtcata ccggagccct ggaaatacag gttttcaccg ccatggtgat
     1381 ggtgatggtg atggtgatgg tgcatggttt cggaccggga tccgtcgccc gatggtggga
     1441 cggtatgaat aatccggaat atttataggt ttttttatta caaaactgtt acgaaaacag
     1501 taaaatactt atttatttgc gagatggtta tcattttaat tatctccatg atctattaat
     1561 attccggagt aggtcgcgaa tcgatactag tgtttaaaca ggtgaaacct tgcgctcgtt
     1621 cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac
     1681 accgtggaaa cggatgaagg cacgaaccca gttgacataa gcctgttcgg ttcgtaaact
     1741 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     1801 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat
     1861 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     1921 cagcaacgat gttacgcagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     1981 agttaggtgg ctcaagtatg ggcatcattc gcacatgtag gctcggccct gaccaagtca
     2041 aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt cggagacgta gccacctact
     2101 cccaacatca gccggactcc gattacctcg ggaacttgct ccgtagtaag acattcatcg
     2161 cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct cgcggcttac gttctgccca
     2221 agtttgagca gccgcgtagt gagatctata tctatgatct cgcagtctcc ggcgagcacc
     2281 ggaggcaggg cattgccacc gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg
     2341 gtgcttatgt gatctacgtg caagcagatt acggtgacga tcccgcagtg gctctctata
     2401 caaagttggg catacgggaa gaagtgatgc actttgatat cgacccaagt accgccacct
     2461 aacaattcgt tcaagccgag atcggcttcc cggccgcgga gttgttcggt aaattgtcac
     2521 aacgccgcga atatagtctt taccatgccc ttggccacgc ccctctttaa tacgacgggc
     2581 aatttgcact tcagaaaatg aagagtttgc tttagccata acaaaagtcc agtatgcttt
     2641 ttcacagcat aactggactg atttcagttt acaactattc tgtctagttt aagactttat
     2701 tgtcatagtt tagatctatt ttgttcagtt taagacttta ttgtccgccc acacccgctt
     2761 acgcagggca tccatttatt actcaaccgt aaccgatttt gccaggttac gcggctggtc
     2821 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     2881 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2941 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3001 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3061 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3121 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3181 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3241 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3301 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3361 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3421 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3481 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3541 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3601 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3661 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3721 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     3781 aacgggaaac gtcttgctct aggccgcgat taaattccaa catggatgct gatttatatg
     3841 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
     3901 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     3961 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     4021 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa
     4081 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     4141 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     4201 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     4261 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     4321 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     4381 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     4441 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     4501 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     4561 tgatgctcga tgagtttttc taagaattct tcacgaccac gctgatgagc tttaccgcag
     4621 tacgtttggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     4681 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     4741 cgctacaggg cgcgtcccat tcgccattca ggctgcaaat aagcgttgat attcagtcaa
     4801 ttacaaacat taataacgaa gagatgacag aaaaattttc attctgtgac agagaaaaag
     4861 tagccgaaga tgacggtttg tcacatggag ttggcaggat gtttgattaa aaacataaca
     4921 ggaagaaaaa tgccccgctg tgggcggaca aaatagttgg gaactgggag gggtggaaat
     4981 ggagttttta aggattattt agggaagagt gacaaaatag atgggaactg ggtgtagcgt
     5041 cgtaagctaa tacgaaaatt aaaaatgaca aaatagtttg gaactagatt tcacttatct
     5101 ggttgtttaa acataccaga acatcttgcc tactcagcct agtccaatta gatgggtata
     5161 ccctaggggt tatgatagtt attgctcagc ggtggcagca gccaactcag cttcctttcg
     5221 ggctttgtta gcagccggat cttctaggct caagcagtga tcagatccag acatgataag
     5281 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     5341 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     5401 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     5461 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag tacttctcga
     5521 caagcttggt accgcatgtc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
     5581 atattcccca gaacatcagg ttaatggcgt tacttctcga actgcggatg cgaccaggcc
     5641 gagccgctgc cgccgccctg aaaatacagg ttttcgccgc tgccgccctt gtacagctcg
     5701 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     5761 ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt gtcgggcagc
     5821 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     5881 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
     5941 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     6001 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     6061 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     6121 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     6181 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
     6241 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     6301 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     6361 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatgccgcc gctgccgccg
     6421 tattgagccc agagcaccgc cttacagccc ttatccactg aaaccacatg cgaaccgctg
     6481 ggggaccagg ccacagcgtt gatagagctg gagtgctgct tggacaggac cttctccacc
     6541 ttgccagtca gcacagacca gatgtacagt gagccttcag cagaaccggc agcgacgtag
     6601 ctaccgtctg gggagaagac cactctggtc cagtctgatc cgcacttgaa gccgggagca
     6661 gagaaagtct gcttgatggc gttggtgcgc aggtcgatga ccttcagcag gtcgtcacgt
     6721 gagcaagaca gcagttcagt gcgctcaggg ttcaggtcca gagcggtgat ctttcccagc
     6781 agttccatct cacggacgat gctctcggac ctgatgtccc agaaacggat cttcttgtcg
     6841 aagtgtccag acatcacgca ctgttcagtg cagacgatgt cgttgcatga agagccggcg
     6901 aacacggtct tgatgcagac cttgcttctc aggtcccaca gcttcagggt cctgtcgtgt
     6961 gagccagaca cgatacgagc gttgtccagc aggaacttgg ctgacaggac cttgccagag
     7021 tgtccagtca gggtgtggcg cagtctgtag tcgtccacag tccagatcct gctagcgaag
     7081 tcgttggagg cagccagcag gtagctacca gcggagtcga actcgataga ggtgataccg
     7141 gcgttgcttc cggacagtga gcccttgaac tcgcacttct ctccgaacac ttcccacagc
     7201 ttgaccctac ggtccatacc tccggtggcc agcagacgtg aaccaggaga gaactggaca
     7261 gcgttcacct ctccgtcgtg ggcgtcgaag acgcacagag cagtagcggg gacgcgcact
     7321 tcctttccgc tgccagggtg ggtgtccacg ttgtcctgag ggactgggaa gctggacaca
     7381 gagcgtctac cgaagatgtt agtgatgctg tccagcaggc caccagcagg ctgggacagc
     7441 ctcttggtag cagcgcggct gatggctctc actggggaag tttcctcggt gtggtcggaa
     7501 gtctcgtcca cgatcacttc gatgtcgtcg tcctgctcga cgggcagagg ttccttggca
     7561 gcttcagcca gctccttttg cagtctggcc tgcctacggc gtgagtcctt ttcgttctca
     7621 gcgttcagtc tgttagcttc ctgggccttc tcagccatcc acctggtcac cagctcctgg
     7681 ttttcctcag tggtcttcct cagcttgcct tccagagcag tgaaggtgat ttgcagggcg
     7741 tcgtactcgt ccttcagagt ctggttggcg cgttccaggt cgcacagctt ggttctcagg
     7801 tccaggcatt cagtctccag gtcggagatg gtttgcaggc actcggcgat cttagcttcg
     7861 ttcatctgca tctccctgtc cttacgctgc atctggttgt tcaggtcgat gaccagctga
     7921 gccagctcac cacgcttctt gtgcagttca gtcagttcct cctggtgctt gatgcgcagc
     7981 tgggccatct cttgcagctg gttgtcgttc caggtgccgt cgtgaccagg gctgatctcg
     8041 tggcggttgg gcacgtcgtg cttttcagct tgcagcttct gggccaggac tgagtgcagg
     8101 tcagacttct ccagcagctt gttgtattgc aggatgattt cctcgaaagc ctgtctctgc
     8161 aatctatctc ttctacgcaa ctgttcgctg atgtgacgct tccacctggg gaaatccgcc
     8221 gccctcaaac cgctgctcat ggtttcggac cgggatccgt cgcccgatgg tgggacggta
     8281 tgaataatcc ggaatattta taggtttttt tattacaaaa ctgttacgaa aacagtaaaa
     8341 tacttattta tttgcgagat ggttatcatt ttaattatct ccatgatcta ttaatattcc
     8401 ggagtaggtc gcgaatcgat actagtgttt aaacgcagcc aattagatgg gtatacccta
     8461 ggggttatga tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
     8521 tgttagcagc cggatcttct aggctcaagc agtgatcaga tccagacatg ataagataca
     8581 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     8641 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     8701 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     8761 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagtactt ctcgacaagc
     8821 ttggtaccgc atgtcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttatatt
     8881 ccccagaaca tcaggttaat ggcgttagcc ccacgcttgt gatttgcagt aatggagaac
     8941 caacttgccg tcgcttccga aacactcgta cagagtaccc acttcctggt caggagatgg
     9001 agcgaaggac tggttcacgt agatgaacag ctgttcagaa gccaccagct tcaggaactt
     9061 cttgatgaag tcgatcagac cctggatagt acgggtcctc tccacagccc acttcttggt
     9121 cttcatgata ggagtgtcac cgacagcctt cagcaggatg tcgatcttct tcttagtgtc
     9181 accagcaggc tcttcagtac caggtgagac agcggctgaa gaaggtggct caggagtggt
     9241 agtctcgggg ctaacatctg tgagtccttc gcctcctgcg gcaatgcttg tgggcaactg
     9301 caaaacgctc tggggctctt cggccatggt ttcggaccgg gatccgtcgc ccgatggtgg
     9361 gacggtatga ataatccgga atatttatag gtttttttat tacaaaactg ttacgaaaac
     9421 agtaaaatac ttatttattt gcgagatggt tatcatttta attatctcca tgatctatta
     9481 atattccgga gtaggtcgcg aatcgatact agtgtttaaa cctgaccaat tagatgggta
     9541 taccctaggg gttatgatag ttattgctca gcggtggcag cagccaactc agcttccttt
     9601 cgggctttgt tagcagccgg atcttctagg ctcaagcagt gatcagatcc agacatgata
     9661 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     9721 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     9781 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     9841 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct agtacttctc
     9901 gacaagcttg gtaccgcatg tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc
     9961 ttatattccc cagaacatca ggttaatggc gttagggcac gtttctttcg tcttggctcg
    10021 tcgcctttgc gtaggacaat gggaggttga ggccgaccac aggaccgacg atgctcagcc
    10081 aggaggtgat gtagttcacg ttcttgttga tcttctgaga gttcttcagg actggagtca
    10141 tgaactcgtt ggtcttgcag gggtgcagca cgaagaaggg ctgacccagg atagggtgct
    10201 cctgctgagt gatggtgtcc caaggtcctt gcagcaggcg catcttgtag cattcgtgca
    10261 ctccctccca gatgtccttc agagtcaggg gacggccgtc caggaagctg gccctgaagt
    10321 acaggacagg cacctggtag gagcaggagt acaggacgtg gtattcgtac ttgatgacct
    10381 cggaagcggc agcggtttcg atgacctcgc agtcgtccag aggcagctcg aaagcctcct
    10441 ccattggcag gcaagtctga ccgtgggtgg aggctcccag gtgtgacatc acagagccgt
    10501 tcttgatctg gaagtggatc ttgcacatgt aaccgtctga gcagtccttg gaagggcgcc
    10561 actcccaact atctccgatt tgctgggaat gtttgatgaa ctccgcacag tatctttgga
    10621 acgtcttttc tccgatgaac tcgtcctcct ccatggtttc ggaccgggat ccgtcgcccg
    10681 atggtgggac ggtatgaata atccggaata tttataggtt tttttattac aaaactgtta
    10741 cgaaaacagt aaaatactta tttatttgcg agatggttat cattttaatt atctccatga
    10801 tctattaata ttccggagta ggtcgcgaat cgatactagt gtttaaacag ccccaattag
    10861 atgggtatac cctaggggtt atgatagtta ttgctcagcg gtggcagcag ccaactcagc
    10921 ttcctttcgg gctttgttag cagccggatc ttctaggctc aagcagtgat cagatccaga
    10981 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
    11041 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
    11101 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
    11161 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctagt
    11221 acttctcgac aagcttggta ccgcatgtca gtggtggtgg tggtggtgct cgagtgcggc
    11281 cgcaagctta tattccccag aacatcaggt taatggcgtt aaatagtctc atcgtcgctc
    11341 atatcccaaa tctcagccgc ctgcgtttcc tggtggagca gagtcagtcc ggtcaggtct
    11401 tccaggaagc tgtgtgaaga gttgaacacc ttagccagga agttgaagcc ttcgcgctcg
    11461 tactggtcca ggaccttgct ggagcaggcg gtgcacttgt cgaaagccag gctgacgggc
    11521 agcacgttgt cgaaacggga caggaatccg cggatctggt gaggcaccag gcccagtgaa
    11581 gtgggaggct cgttcattct gtcgtctgaa gagctggcga tagcgtaacc tccttctggg
    11641 tgttgcagga cagacaccat cagctccaca gccagagcgc cagcgatgac agccagaccg
    11701 ggccttgaca cggtgcactg ctggtccaga gtacggtcgc gggtagagtc gcctggggcc
    11761 accacgtcgt tgcagaagta gcaacccagc ttgtatccgg ggatgttagc gaacagggat
    11821 gaacccagca ggtcagcaga ggcgactggg tggttggggc acaggtcacc agctccctgc
    11881 tgcttgggct tcttcagacc gtgacgcatg accacgaagg tgtcgaatcc cagagcggcg
    11941 ttgatgacca gcttgcgctt tgaagcagcg atgacggcag gcagccagcg ggattcccta
    12001 gtgtccatca gcaggaagac cacgtcgtgg ctctcgatca gctgttccag ctgctccacg
    12061 tccctgcgag cctgctccag ggtgacagag ctgaagttca cagggtgacc tggcatgggg
    12121 atggacatgt tgaatcctct ggcgttgaca ccagggaaaa tcttttgcag cctgtcagcg
    12181 gcagccagag cctttggctt gccacctccc aggcagtcct cgaactcgta cagaggctga
    12241 cgcactgggt tgctgtagct gatcttggcg ttgtccacga aggtgatgtg tctcacgccc
    12301 caacccatca gagtcctggc gacgttgcat cccagggttc cagcacccag cagcaggcac
    12361 ttcacggaca ccaccttgtc caggtccaga gtggggacca gtctccagca catcagcttc
    12421 aggttcaggt ccacggatga ttcagccaga cgctttgggt ccatgcactc agacaggttc
    12481 accatgcggg ggcccatgcc acccttctgg ttcttttccc aaccgacggc cttagggcag
    12541 tctgggctga aagccatttc aggcagcttc acctcgaaga tgatggagtg ggcgacgtcc
    12601 ctagcaccct gcatggttct gtccctgaag cagaccacct ccacggactg gaaagagctg
    12661 gaccaacggt gggcagccag gaccaggaag ttgcgcagtg gccatccagg gtactgagcg
    12721 aggttgcaag ggtcgtacac accgatagtg atcttggtac gctgtccctg gaagaagtct
    12781 gagtagtgct tcagcaggga caccagcacc atgttttcgt cgtacttgat caggaagtat
    12841 ggcagagcag tcacaccctc ggtctggcac aggttgtcgt aggcgcattc cagagcctcg
    12901 atctgcttca ggctgaacct ctggtccagt ccgacaggtc cttggatcag tggcagggat
    12961 tcaggcaggc acagggcagg gtagcagaac cagtagtaga agtggtactt cttcaggtca
    13021 gcgaaagtca gcagcaggaa cttgttcagc agcactgggt tctccagggc ggttcctgac
    13081 ttgatagatt cccagatttc gttggcagcc tgttccagca gcagcttctt gtcagcagtc
    13141 ttgaagctct ccagagtgtt ggtgttgtac agagtgccga tagctgggca gcatctggct
    13201 ggagttggag cgctcatgtc gaaagcggag aactccaggg tcagcctggc aggcaggcca
    13261 gcggagtcac cgttgtagta gtagcccttg atgtcctttg gagcttcgtc cagcctgtac
    13321 tcgttcagct tcttctgagt cagttcatgc cagaatccga catcgagtgc cgaggagaac
    13381 ggggcgaatt ggagtttaga caagccaggg tcaccagtag ccgctgccat ggtttcggac
    13441 cgggatccgt cgcccgatgg tgggacggta tgaataatcc ggaatattta taggtttttt
    13501 tattacaaaa ctgttacgaa aacagtaaaa tacttattta tttgcgagat ggttatcatt
    13561 ttaattatct ccatgatcta tta
//
