LOCUS       sequence-332087-       10709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for FLCN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, 
            Lim CY, Samelson AJ, Hurley JH, Zoncu R
  TITLE     Structural mechanism of a Rag GTPase activation checkpoint by the 
            lysosomal folliculin complex.
  JOURNAL   Science. 2019 Nov 22;366(6468):971-977. doi: 
            10.1126/science.aax0364. Epub 2019 Oct 31.
  PUBMED    31672913
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax0364"; journalName: "Science"; date: 
            "2019-11-22- 22"; volume: "366"; issue: "6468"; pages: "971-977"
COMMENT     Sequence Label: pLJM1 FLCN (WT) FLAG GFP
FEATURES             Location/Qualifiers
     source          1..10709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     59..79
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        250..1461
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1298..1317
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3223..3246
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(3271..3273,3274..3987)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3271..3273,3274..3984)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3313..3334)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3574..3593)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3921..3942
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4029..4146
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4195..4705
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4624..4642
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4727..5326
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4727..4746)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5223..5243
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5454..5687
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5759..5880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5796..5815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5850..5869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5920..6055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6075..6094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6076..6094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6104..6121)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6104..6120)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6113..6135)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6262..6717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6349..6368)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6559..6580
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6743..6847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6848..6916,6917..7708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7066..7085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7879..8467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8368..8387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8621..8638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8755..8776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8791..8796,8797..8814,8815..8821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8829..8845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8834..8856
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8853..8869
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8853..8869
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8888..8908
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8890..8908
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8936..9162
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9163..9343
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9390..9515
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10008..10241
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10426..10470
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaacggatct cgacggtatc gatcacgaga ctagcctcga gcggccgccc ccttcaccga
       61 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
      121 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
      181 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      241 gggtgcagcg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
      301 cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc
      361 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg
      421 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa
      481 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg
      541 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg
      601 gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc
      661 tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg
      721 agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg
      781 gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc
      841 gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct
      901 tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct
      961 ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg
     1021 ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct
     1081 cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg
     1141 gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct
     1201 ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt
     1261 ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg
     1321 cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca
     1381 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     1441 tttttggctt ttttgttaga cgctagcgct accggtgcca ccatgatgaa cgccatcgtg
     1501 gccctgtgcc acttctgtga gctgcacggc ccacggaccc tgttttgcac agaggtgctg
     1561 cacgcaccac tgccacaggg cgacggaaac gaggatagcc caggacaggg agagcaggca
     1621 gaggaggagg agggcggcat ccagatgaat agccggatga gagcccactc cccagcagag
     1681 ggagcctctg tggagagctc ctctcctggc ccaaagaaga gcgacatgtg cgagggatgt
     1741 cggtccctgg ccgcaggaca ccctggctat atctctcacg ataaggagac aagcatcaag
     1801 tacgtgtctc accagcaccc cagccaccct cagctgttct ccatcgtgcg gcaggcctgc
     1861 gtgagatccc tgtcttgcga ggtgtgccca ggaagagagg gacctatctt ctttggcgac
     1921 gagcagcacg gcttcgtgtt ttctcacacc ttctttatca aggatagcct ggccaggggc
     1981 ttccagcggt ggtattccat catcacaatc atgatggacc ggatctacct gatcaactct
     2041 tggccctttc tgctgggcaa ggtgagaggc atcatcgacg agctgcaggg caaggccctg
     2101 aaggtgttcg aggccgagca gtttggctgt ccacagaggg cccagaggat gaacaccgcc
     2161 ttcacacctt ttctgcacca gaggaacggc aatgccgccc gctctctgac cagcctgaca
     2221 tccgacgata atctgtgggc ctgcctgcac accagctttg cctggctgct gaaggcctgt
     2281 ggctccaggc tgacagagaa gctgctggag ggcgccccta ccgaggacac actggtgcag
     2341 atggagaagc tggccgacct ggaggaggag agcgagtcct gggataatag cgaggcagaa
     2401 gaggaggaga aggcaccagt gctgccagag tccaccgagg gaagggagct gacacaggga
     2461 ccagccgaga gctcctctct gtctggctgc ggcagctggc agcctagaaa gctgccagtg
     2521 ttcaagtctc tgaggcacat gagacaggtg ctgggcgccc ccagctttag gatgctggcc
     2581 tggcacgtgc tgatgggcaa ccaggtcatc tggaagtccc gcgacgtgga tctggtgcag
     2641 tctgccttcg aggtgctgag gaccatgctg ccagtgggct gcgtgcgcat catcccctac
     2701 agctcccagt atgaggaggc ctacaggtgt aatttcctgg gcctgagccc tcacgtgcag
     2761 atcccacctc acgtgctgtc tagcgagttt gccgtgatcg tggaggtgca cgccgccgcc
     2821 cggagcaccc tgcacccagt gggctgtgag gacgatcagt ctctgagcaa gtatgagttt
     2881 gtggtgacat ccggctctcc tgtggcagca gacagggtgg gcccaaccat cctgaacaag
     2941 atcgaggccg ccctgacaaa ccagaatctg agcgtggacg tggtggatca gtgcctggtg
     3001 tgcctgaagg aggagtggat gaataaggtg aaggtgctgt tcaagtttac caaggtggac
     3061 tcccgcccta aggaggatac acagaagctg ctgtccatcc tgggcgcctc tgaggaggat
     3121 aacgtgaagc tgctgaagtt ctggatgacc ggcctgagca agacatacaa gtcccacctg
     3181 atgtctaccg tgcggagccc aacagccagc gagtccagaa atgactacaa agacgatgac
     3241 gacaagggta gcggtagtgg aagcgtcgac gtgagcaagg gcgaggagct gttcaccggg
     3301 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3361 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3421 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3481 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3541 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3601 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3661 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3721 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     3781 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3841 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     3901 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3961 ctcggcatgg acgagctgta caagtgagaa ttctcgacct cgagacaaat ggcagtattc
     4021 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     4081 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     4141 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt
     4201 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
     4261 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
     4321 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
     4381 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
     4441 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
     4501 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
     4561 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
     4621 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
     4681 ccgaatcacc gacctctctc cccaggggga tccaccggag cttaccatga ccgagtacaa
     4741 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     4801 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     4861 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     4921 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     4981 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5041 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5101 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5161 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5221 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5281 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     5341 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcggtacctt taagaccaat
     5401 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5461 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     5521 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5581 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5641 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     5701 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     5761 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5821 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5881 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     5941 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6001 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     6061 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     6121 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6181 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6241 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6301 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6361 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6421 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6481 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6541 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6601 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6661 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     6721 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6781 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6841 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6901 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6961 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7021 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7081 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7141 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7201 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7261 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7321 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7381 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7441 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7501 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7561 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7621 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7681 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7741 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7801 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7861 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7921 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7981 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8041 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8101 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8161 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8221 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8281 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8341 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8401 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8461 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8521 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8581 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8641 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     8701 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     8761 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     8821 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     8881 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     8941 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     9001 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     9061 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     9121 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     9181 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9241 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9301 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     9361 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     9421 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     9481 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     9541 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     9601 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     9661 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     9721 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     9781 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     9841 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     9901 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     9961 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    10021 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    10081 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    10141 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    10201 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    10261 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    10321 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    10381 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    10441 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    10501 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    10561 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    10621 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    10681 agagacagag acagatccat tcgattagt
//
