LOCUS       sequence-332097-        8433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET fusion between ymTq2 and yomCherry for use in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8433)
  TITLE     Teusink unpublished plasmids
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ymTq2-yomCherry pDRF1-GW
FEATURES             Location/Qualifiers
     source          1..8433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    665..689
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1463..1472
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2188..2212)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2274..2293)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2275..2293)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2441..2628
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2496..2520)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2640..2657)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2640..2656)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2649..2671)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3092..4438
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4549..5352)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5353..5568)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5593..5611)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5679..5783
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5784..5852,5853..6644)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6002..6021)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6815..7403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7304..7323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7557..7574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7691..7712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7727..7732,7733..7750,7751..7757)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7765..7781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7770..7792
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7789..7805
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7789..7805
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7835..8290)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7972..7993)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8184..8203
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaaataaacc catacacgag acgtagaacc agccgcacat ggccggagaa actcctgcga
       61 gaatttcgta aactcgcgcg cattgcatct gtatttccta atgcggcact tccaggcctc
      121 gatcgagacc gtttatccat tgcttttttg ttgtcttttt ccctcgttca cagaaagtct
      181 gaagaagcta tagtagaact atgagctttt tttgtttctg ttttcctttt tttttttttt
      241 acctctgtgg aaattgttac tctcacactc tttagttcgt ttgtttgttt tgtttattcc
      301 aattatgacc ggtgacgaaa cgtggtcgat ggtgggtacc gcttatgctc ccctccatta
      361 gtttcgatta tataaaaagg ccaaatattg tattattttc aaatgtccta tcattatcgt
      421 ctaacatcta atttctctta aattttttct ctttctttcc tataacacca atagtgaaaa
      481 tctttttttc ttctatatct acaaaaactt tttttttcta tcaacctcgt tgataaattt
      541 tttctttaac aatcgttaat aattaattaa ttggaaaata accatttttt ctctctttta
      601 tacacacatt caaaagaaag aaaaaaaata taccccagcc tcgactagtg aattcgcggc
      661 catcacaagt ttgtacaaaa aagcaggctg ctagcactag taagctttta attaaatggt
      721 tagtaaaggt gaagaattgt tcactggtgt agtgccaatt ttagtagaat tagatggtga
      781 cgttaacggt cataagttct ctgtaagcgg tgaaggtgaa ggtgacgcaa catacggtaa
      841 attaactttg aaattcattt gtactacagg taaattacca gttccatggc caactttggt
      901 tactactttg tcttggggtg ttcaatgttt cgctagatac ccagatcata tgaagcaaca
      961 tgatttcttt aagtctgcta tgccagaagg ttacgttcaa gaaagaacta tcttcttcaa
     1021 ggatgatggt aactataaga ctagagctga agttaagttc gaaggagata ctttagttaa
     1081 tagaattgaa ttgaagggta ttgattttaa ggaagatggt aatattttgg gtcataagtt
     1141 ggaatataat tacttctctg ataacgttta cattactgct gataagcaaa agaacggtat
     1201 taaggcgaac ttcaagatta gacataacat tgaagatggt ggtgttcaat tagctgatca
     1261 ttaccaacaa aacactccaa ttggtgacgg tccagttttg ttgccagata accattactt
     1321 gtctactcaa tctaagttgt ctaaggatcc aaacgaaaag agagatcata tggttttgtt
     1381 ggaattcgtt actgctgctg ggatcacttt aggtatggat gaattgtata aatccggact
     1441 cagatcttcg gatccaccgg tcgccaccat ggttagcaaa ggcgaggaag ataacatggc
     1501 tataatcaaa gagtttatga gattcaaagt acacatggag ggttcagtga atggtcatga
     1561 atttgaaatt gaaggcgaag gcgagggcag accttacgaa ggaactcaaa cagcaaaact
     1621 taaggtaaca aaaggtggtc ctctgccatt cgcctgggac attctcagtc cacaattcat
     1681 gtacggttct aaagcgtacg tcaaacatcc agcagacatt ccagattact tgaaattgtc
     1741 ttttccagaa ggctttaagt gggaaagagt tatgaacttc gaggatggag gggttgtgac
     1801 cgttacgcaa gattcctctt tacaagatgg tgagtttatc tacaaggtca aattaagggg
     1861 gactaatttt ccttcagacg ggccagtcat gcagaaaaag actatgggat gggaagcctc
     1921 ttcagagaga atgtatcctg aagatggcgc tctaaaagga gaaatcaagc aaagattgaa
     1981 gttaaaggac ggaggtcatt atgatgcaga agtaaaaaca acctataaag ctaaaaagcc
     2041 agttcaactt cctggtgcct acaatgttaa catcaagcta gacattacat cccataatga
     2101 agattacact atagtggaac agtatgaacg tgctgaaggt agacacagta caggtggtat
     2161 ggatgaactg tacaagtaag cggccgcacc cagctttctt gtacaaagtg gtgtagttaa
     2221 ttcatgatgg ccgctgcagg tcgacctcga gggggggccc ggtacccaat tcgccctata
     2281 gtgagtcgta ttacgcgcgg atccagcttt ggacttcttc gccagaggtt tggtcaagtc
     2341 tccaatcaag gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg
     2401 tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat
     2461 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
     2521 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
     2581 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccaattca
     2641 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2701 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     2761 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     2821 acgcatctgt gcggtatttc acaccgcata atcggatcgt acttgttacc catcattgaa
     2881 ttttgaacat ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata
     2941 ataatatata gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac
     3001 tattgcatct attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt
     3061 ccatcttgca cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga
     3121 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     3181 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
     3241 gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     3301 tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
     3361 tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
     3421 actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
     3481 gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
     3541 tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
     3601 aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa
     3661 gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
     3721 ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
     3781 aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat
     3841 gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag
     3901 ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt
     3961 attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg
     4021 tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag
     4081 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
     4141 caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
     4201 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
     4261 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
     4321 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
     4381 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcga
     4441 tccgatgata agctgtcaaa catgagaatt gggtaataac tgatataatt aaattgaagc
     4501 tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg
     4561 gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt
     4621 agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga
     4681 gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc
     4741 cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg
     4801 agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt
     4861 atattctcca gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct
     4921 aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac
     4981 cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta
     5041 ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt
     5101 gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa
     5161 gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag
     5221 taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat
     5281 gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt
     5341 agctttcgac atgatttatc ttcgtttcct gcatgttttt gttctgtgca gttgggttaa
     5401 gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag
     5461 tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca
     5521 aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctaattctt
     5581 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5641 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5701 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5761 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5821 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5881 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5941 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6001 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6061 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6121 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6181 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6241 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6301 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6361 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6421 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6481 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6541 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6601 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6661 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6721 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6961 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7441 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7501 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7561 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7621 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7681 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7741 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7801 atgaccatga ttacgccaag cttaccgcat caggaaattg taagcgttaa tattttgtta
     7861 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     7921 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     7981 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     8041 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     8101 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     8161 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     8221 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     8281 cagggcgcgt ccattcgcca agcttcctga aacggagaaa cataaacagg cattgctggg
     8341 atcacccata catcactctg ttttgcctga ccttttccgg taatttgaaa acaaacccgg
     8401 tctcgaagcg gagatccggc gataattacc gca
//
