LOCUS       sequence-332099-        7664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses yomCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7664)
  TITLE     Teusink unpublished plasmids
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: yomCherry pDRF1-GW
FEATURES             Location/Qualifiers
     source          1..7664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    204..228
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      233..242
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(958..982)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1044..1063)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1045..1063)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1211..1398
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1266..1290)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1410..1427)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1410..1426)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1419..1441)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1862..3208
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3319..4122)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4123..4338)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(4363..4381)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4449..4553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4554..4622,4623..5414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4772..4791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5585..6173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6074..6093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6327..6344
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6461..6482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6497..6502,6503..6520,6521..6527)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6535..6551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6540..6562
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6559..6575
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6559..6575
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(6605..7060)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6742..6763)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6954..6973
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ataacaccaa tagtgaaaat ctttttttct tctatatcta caaaaacttt ttttttctat
       61 caacctcgtt gataaatttt ttctttaaca atcgttaata attaattaat tggaaaataa
      121 ccattttttc tctcttttat acacacattc aaaagaaaga aaaaaaatat accccagcct
      181 cgactagtga attcgcggcc atcacaagtt tgtacaaaaa agcaggctgc tagccaccat
      241 ggttagcaaa ggcgaggaag ataacatggc tataatcaaa gagtttatga gattcaaagt
      301 acacatggag ggttcagtga atggtcatga atttgaaatt gaaggcgaag gcgagggcag
      361 accttacgaa ggaactcaaa cagcaaaact taaggtaaca aaaggtggtc ctctgccatt
      421 cgcctgggac attctcagtc cacaattcat gtacggttct aaagcgtacg tcaaacatcc
      481 agcagacatt ccagattact tgaaattgtc ttttccagaa ggctttaagt gggaaagagt
      541 tatgaacttc gaggatggag gggttgtgac cgttacgcaa gattcctctt tacaagatgg
      601 tgagtttatc tacaaggtca aattaagggg gactaatttt ccttcagacg ggccagtcat
      661 gcagaaaaag actatgggat gggaagcctc ttcagagaga atgtatcctg aagatggcgc
      721 tctaaaagga gaaatcaagc aaagattgaa gttaaaggac ggaggtcatt atgatgcaga
      781 agtaaaaaca acctataaag ctaaaaagcc agttcaactt cctggtgcct acaatgttaa
      841 catcaagcta gacattacat cccataatga agattacact atagtggaac agtatgaacg
      901 tgctgaaggt agacacagta caggtggtat ggatgaactg tacaagtaag cggccgcacc
      961 cagctttctt gtacaaagtg gtgtagttaa ttcatgatgg ccgctgcagg tcgacctcga
     1021 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgg atccagcttt
     1081 ggacttcttc gccagaggtt tggtcaagtc tccaatcaag gttgtcggct tgtctacctt
     1141 gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac
     1201 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     1261 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
     1321 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
     1381 tgaccacacc tctaccggca tgccaattca ctggccgtcg ttttacaacg tcgtgactgg
     1441 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     1501 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1561 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     1621 atcggatcgt acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc
     1681 tgaaacagat agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga
     1741 caatgtatgt atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca
     1801 cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt
     1861 taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
     1921 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
     1981 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
     2041 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
     2101 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
     2161 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
     2221 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
     2281 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
     2341 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
     2401 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
     2461 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
     2521 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
     2581 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
     2641 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
     2701 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
     2761 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
     2821 ctctgaagtt cctatacttt ctagctagag aataggaact tcggaatagg aacttcaaag
     2881 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     2941 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     3001 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
     3061 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
     3121 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
     3181 atgctatcat ttcctttgat attggatcga tccgatgata agctgtcaaa catgagaatt
     3241 gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt
     3301 tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc
     3361 tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct
     3421 tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg
     3481 acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta
     3541 accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc
     3601 gctcttcgca atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca
     3661 tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg
     3721 cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca
     3781 ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc
     3841 aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt
     3901 aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat
     3961 tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga
     4021 agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact
     4081 aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct
     4141 gcatgttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac
     4201 actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg
     4261 ttcggagatt accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa
     4321 aaaaaatgat gaattgaaaa gctaattctt gaagacgaaa gggcctcgtg atacgcctat
     4381 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4441 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4501 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4561 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4621 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4681 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4741 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4801 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4861 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4921 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4981 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5041 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5101 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5161 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5221 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5281 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5341 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5401 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5461 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5521 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5581 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5641 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5701 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5761 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5821 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5881 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5941 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6001 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6061 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6121 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6181 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6241 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6301 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6361 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6421 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6481 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6541 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cttaccgcat
     6601 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     6661 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     6721 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     6781 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     6841 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     6901 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     6961 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     7021 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca agcttcctga
     7081 aacggagaaa cataaacagg cattgctggg atcacccata catcactctg ttttgcctga
     7141 ccttttccgg taatttgaaa acaaacccgg tctcgaagcg gagatccggc gataattacc
     7201 gcagaaataa acccatacac gagacgtaga accagccgca catggccgga gaaactcctg
     7261 cgagaatttc gtaaactcgc gcgcattgca tctgtatttc ctaatgcggc acttccaggc
     7321 ctcgatcgag accgtttatc cattgctttt ttgttgtctt tttccctcgt tcacagaaag
     7381 tctgaagaag ctatagtaga actatgagct ttttttgttt ctgttttcct tttttttttt
     7441 tttacctctg tggaaattgt tactctcaca ctctttagtt cgtttgtttg ttttgtttat
     7501 tccaattatg accggtgacg aaacgtggtc gatggtgggt accgcttatg ctcccctcca
     7561 ttagtttcga ttatataaaa aggccaaata ttgtattatt ttcaaatgtc ctatcattat
     7621 cgtctaacat ctaatttctc ttaaattttt tctctttctt tcct
//
