LOCUS       sequence-332110-        8392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET fusion between ymScarletI and ymNeongreen for use in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8392)
  TITLE     Teusink unpublished plasmids
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ymScarletI-ymNeongreen pDRF1-GW
FEATURES             Location/Qualifiers
     source          1..8392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(233..688)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(370..391)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     582..601
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    1496..1520
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2253..2262
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2514..2523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2978..3002)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3064..3083)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3065..3083)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3231..3418
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3286..3310)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3430..3447)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3430..3446)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3439..3461)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3882..5228
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5339..6142)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6143..6358)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6383..6401)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6469..6573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6574..6642,6643..7434)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6792..6811)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7605..8193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8094..8113
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8347..8364
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagct taccgcatca ggaaattgta
      241 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
      301 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
      361 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
      421 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
      481 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
      541 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
      601 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
      661 gcgcttaatg cgccgctaca gggcgcgtcc attcgccaag cttcctgaaa cggagaaaca
      721 taaacaggca ttgctgggat cacccataca tcactctgtt ttgcctgacc ttttccggta
      781 atttgaaaac aaacccggtc tcgaagcgga gatccggcga taattaccgc agaaataaac
      841 ccatacacga gacgtagaac cagccgcaca tggccggaga aactcctgcg agaatttcgt
      901 aaactcgcgc gcattgcatc tgtatttcct aatgcggcac ttccaggcct cgatcgagac
      961 cgtttatcca ttgctttttt gttgtctttt tccctcgttc acagaaagtc tgaagaagct
     1021 atagtagaac tatgagcttt ttttgtttct gttttccttt tttttttttt tacctctgtg
     1081 gaaattgtta ctctcacact ctttagttcg tttgtttgtt ttgtttattc caattatgac
     1141 cggtgacgaa acgtggtcga tggtgggtac cgcttatgct cccctccatt agtttcgatt
     1201 atataaaaag gccaaatatt gtattatttt caaatgtcct atcattatcg tctaacatct
     1261 aatttctctt aaattttttc tctttctttc ctataacacc aatagtgaaa atcttttttt
     1321 cttctatatc tacaaaaact ttttttttct atcaacctcg ttgataaatt ttttctttaa
     1381 caatcgttaa taattaatta attggaaaat aaccattttt tctctctttt atacacacat
     1441 tcaaaagaaa gaaaaaaaat ataccccagc ctcgactagt gaattcgcgg ccatcacaag
     1501 tttgtacaaa aaagcaggct gctagcatgg ttagtaaagg tgaagctgtt ataaaagaat
     1561 tcatgaggtt taaagttcat atggaaggtt caatgaatgg tcatgaattt gaaattgaag
     1621 gtgaaggtga aggtagacca tatgaaggta cacaaactgc taaattgaaa gttactaaag
     1681 gtggtccatt accattttct tgggatattt tgtctccaca attcatgtat ggttctagag
     1741 cttttattaa gcatccagct gatattccag attattataa acaatctttt cctgaaggtt
     1801 ttaaatggga aagagttatg aatttcgaag atggtggtgc tgttactgtt actcaagata
     1861 cttctttgga agatggtact ttaatctata aagttaaatt gagaggtact aattttccac
     1921 cagatggtcc agttatgcaa aagaaaacta tgggttggga agcatctact gaaagattgt
     1981 atccagaaga tggtgttttg aaaggagaca ttaagatggc tttgagattg aaagatggtg
     2041 gtcggtacct ggccgacttc aagaccacct ataaagctaa aaaaccagtt caaatgccag
     2101 gtgcatataa tgttgataga aagttagata taacgtcgca taacgaggac tatactgtag
     2161 ttgaacaata tgaacgtagt gaaggtagac atagtaccgg aggaatggat gaattgtata
     2221 aatccggact cagatcttcg gatccaccgg tcgccaccat ggtctctaag ggtgaagaag
     2281 ataacatggc ttctttgcca gccacccacg agttgcacat cttcggttcc attaacggtg
     2341 tcgacttcga tatggtcggt caaggtaccg gtaacccaaa cgacggttac gaagaattga
     2401 acttgaagtc taccaagggc gacttgcagt tctccccttg gattttggtt ccacacatcg
     2461 gttacggttt ccaccaatac ttgccatacc cagacggtat gtccccattc caagccgcca
     2521 tggttgacgg ttctggttac caagtccaca gaaccatgca attcgaagac ggtgcttcct
     2581 tgaccgtgaa ctacagatac acctacgaag gttctcacat caagggcgaa gcccaagtta
     2641 agggtaccgg tttccctgct gacggtccag tcatgactaa ctccttgacc gctgctgact
     2701 ggtgtcgttc caagaagacc tacccaaacg ataagaccat catttccact ttcaagtggt
     2761 cctacaccac cggtaacggc aagagatacc gctctaccgc tcgtaccacc tacaccttcg
     2821 ccaagccaat ggctgccaac tacttgaaga accagcctat gtacgtcttc cgtaagaccg
     2881 agctgaagca ttccaagact gaattgaact tcaaggaatg gcaaaaggct ttcaccgacg
     2941 tcatgggtat ggacgaattg tacaagtaag cggccgcacc cagctttctt gtacaaagtg
     3001 gtgtagttaa ttcatgatgg ccgctgcagg tcgacctcga gggggggccc ggtacccaat
     3061 tcgccctata gtgagtcgta ttacgcgcgg atccagcttt ggacttcttc gccagaggtt
     3121 tggtcaagtc tccaatcaag gttgtcggct tgtctacctt gccagaaatt tacgaaaaga
     3181 tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa gcgaatttct
     3241 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     3301 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     3361 tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca
     3421 tgccaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     3481 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     3541 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     3601 ttttctcctt acgcatctgt gcggtatttc acaccgcata atcggatcgt acttgttacc
     3661 catcattgaa ttttgaacat ccgaacctgg gagttttccc tgaaacagat agtatatttg
     3721 aacctgtata ataatatata gtctagcgct ttacggaaga caatgtatgt atttcggttc
     3781 ctggagaaac tattgcatct attgcatagg taatcttgca cgtcgcatcc ccggttcatt
     3841 ttctgcgttt ccatcttgca cttcaatagc atatctttgt taacgaagca tctgtgcttc
     3901 attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     3961 gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg
     4021 cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct
     4081 gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat
     4141 ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc
     4201 atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt
     4261 tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat
     4321 aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt
     4381 ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg
     4441 tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct
     4501 tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat
     4561 tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa
     4621 cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta
     4681 ggttatatag ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg
     4741 tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt
     4801 tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt
     4861 ctagctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct
     4921 tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct
     4981 gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa
     5041 atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat
     5101 attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct
     5161 atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat
     5221 attggatcga tccgatgata agctgtcaaa catgagaatt gggtaataac tgatataatt
     5281 aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt
     5341 agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac
     5401 cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag
     5461 atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt
     5521 catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca
     5581 tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag
     5641 tacccttagt atattctcca gtagataggg agcccttgca tgacaattct gctaacatca
     5701 aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac
     5761 ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac
     5821 ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga
     5881 gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa
     5941 aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa
     6001 ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct
     6061 tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt
     6121 ccttatatgt agctttcgac atgatttatc ttcgtttcct gcatgttttt gttctgtgca
     6181 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata
     6241 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa
     6301 aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa
     6361 gctaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6421 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6481 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6541 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6601 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6661 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6721 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6781 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6841 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6901 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6961 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7021 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7081 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7141 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7201 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7261 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7321 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7381 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7441 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7501 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7561 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7621 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7681 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7741 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7801 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7861 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7921 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7981 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8041 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8101 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8161 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8221 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8281 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8341 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa ac
//
