LOCUS       sequence-332111-        5057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries fucO and aldA under the control of P_gyrA 
            promoter, which is used for study about Improving ethylene glycol 
            utilization in Escherichia coli fermentation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Panda S, Fung V, Zhou J, Liang H, Zhou K
  TITLE     Improving ethylene glycol utilization in Escherichia coli 
            fermentation
  JOURNAL   Biochemical Engineering Journal
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochemical
            Engineering Journal"
COMMENT     Sequence Label: pEG03'
FEATURES             Location/Qualifiers
     source          1..5057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     163..180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     RBS             488..510
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3496..4287
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4478..5057,1..9)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4967..4986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
       61 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      121 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      181 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      241 cgcatatgct ggatccttcc ctcgactcac acttggggag gcgacggctt caaatttagc
      301 gatctcttcg tggtctacgt tatggttttc cggcgatttt tcggcattca ttggcacttc
      361 tactccgtaa ttggcaagac aaacgagtat atcaggcatt ggatgtgaat aaagcgtata
      421 ggtttacctc aaactgcgcg gctgtgttat aatttgcgac ctttgaatcc gggatactag
      481 aaataatttt gtttaacttt aagaaggaga tatacatatg gctaacagaa tgattctgaa
      541 cgaaacggca tggtttggtc ggggtgctgt tggggcttta accgatgagg tgaaacgccg
      601 tggttatcag aaggcgctga tcgtcaccga taaaacgctg gtgcaatgcg gcgtggtggc
      661 gaaagtgacc gataagatgg atgctgcagg gctggcatgg gcgatttacg acggcgtagt
      721 gcccaaccca acaattactg tcgtcaaaga agggctcggt gtattccaga atagcggcgc
      781 ggattacctg atcgctattg gtggtggttc tccacaggat acttgtaaag cgattggcat
      841 tatcagcaac aacccggagt ttgccgatgt gcgtagcctg gaagggcttt ccccgaccaa
      901 taaacccagt gtaccgattc tggcaattcc taccacagca ggtactgcgg cagaagtgac
      961 cattaactac gtgatcactg acgaagagaa acggcgcaag tttgtttgcg ttgatccgca
     1021 tgatatcccg caggtggcgt ttattgacgc tgacatgatg gatggtatgc ctccagcgct
     1081 gaaagctgcg acgggtgtcg atgcgctcac tcatgctatt gaggggtata ttacccgtgg
     1141 cgcgtgggcg ctaaccgatg cactgcacat taaagcgatt gaaatcattg ctggggcgct
     1201 gcgaggatcg gttgctggtg ataaggatgc cggagaagaa atggcgctcg ggcagtatgt
     1261 tgcgggtatg ggcttctcga atgttgggtt agggttggtg catggtatgg cgcatccact
     1321 gggcgcgttt tataacactc cacacggtgt tgcgaacgcc atcctgttac cgcatgtcat
     1381 gcgttataac gctgacttta ccggtgagaa gtaccgcgat atcgcgcgcg ttatgggcgt
     1441 gaaagtggaa ggtatgagcc tggaagaggc gcgtaatgcc gctgttgaag cggtgtttgc
     1501 tctcaaccgt gatgtcggta ttccgccaca tttgcgtgat gttggtgtac gcaaggaaga
     1561 cattccggca ctggcgcagg cggcactgga tgatgtttgt accggtggca acccgcgtga
     1621 agcaacgctt gaggatattg tagagcttta ccataccgcc tggtaaccgt tcatttatca
     1681 caaaaggatt gttcgatgtc agtacccgtt caacatccta tgtatatcga tggacagttt
     1741 gttacctggc gtggagacgc atggattgat gtggtaaacc ctgctacaga ggctgtcatt
     1801 tcccgcatac ccgatggtca ggccgaggat gcccgtaagg caatcgatgc agcagaacgt
     1861 gcacaaccag aatgggaagc gttgcctgct attgaacgcg ccagttggtt gcgcaaaatc
     1921 tccgccggga tccgcgaacg cgccagtgaa atcagtgcgc tgattgttga agaagggggc
     1981 aagatccagc agctggctga agtcgaagtg gcttttactg ccgactatat cgattacatg
     2041 gcggagtggg cacggcgtta cgagggcgag attattcaaa gcgatcgtcc aggagaaaat
     2101 attcttttgt ttaaacgtgc gcttggtgtg actaccggca ttctgccgtg gaacttcccg
     2161 ttcttcctca ttgcccgcaa aatggctccc gctcttttga ccggtaatac catcgtcatt
     2221 aaacctagtg aatttacgcc aaacaatgcg attgcattcg ccaaaatcgt cgatgaaata
     2281 ggccttccgc gcggcgtgtt taaccttgta ctggggcgtg gtgaaaccgt tgggcaagaa
     2341 ctggcgggta acccaaaggt cgcaatggtc agtatgacag gcagcgtctc tgcaggtgag
     2401 aagatcatgg cgactgcggc gaaaaacatc accaaagtgt gtctggaatt ggggggtaaa
     2461 gcaccagcta tcgtaatgga cgatgccgat cttgaactgg cagtcaaagc catcgttgat
     2521 tcacgcgtca ttaatagtgg gcaagtgtgt aactgtgcag aacgtgttta tgtacagaaa
     2581 ggcatttatg atcagttcgt caatcggctg ggtgaagcga tgcaggcggt tcaatttggt
     2641 aaccccgctg aacgcaacga cattgcgatg gggccgttga ttaacgccgc ggcgctggaa
     2701 agggtcgagc aaaaagtggc gcgcgcagta gaagaagggg cgagagtggc gttcggtggc
     2761 aaagcggtag aggggaaagg atattattat ccgccgacat tgctgctgga tgttcgccag
     2821 gaaatgtcga ttatgcatga ggaaaccttt ggcccggtgc tgccagttgt cgcatttgac
     2881 acgctggaag atgctatctc aatggctaat gacagtgatt acggcctgac ctcatcaatc
     2941 tatacccaaa atctgaacgt cgcgatgaaa gccattaaag ggctgaagtt tggtgaaact
     3001 tacatcaacc gtgaaaactt cgaagctatg caaggcttcc acgccggatg gcgtaaatcc
     3061 ggtattggcg gcgcagatgg taaacatggc ttgcatgaat atctgcagac ccaggtggtt
     3121 tatttacagt cttgatgggc tgaagggttt aagcgaagac gaacaataag gcctcccaaa
     3181 tcggggggcc ttttttattg ataacaaaaa ggcaacacta tgacatcgga aaacccgtta
     3241 ctggcgctgc gagagaaaat cagcgcgctg gatgaaaaat tattagcgtt actggcagag
     3301 cggcgcgaat tcaaacagaa aggccatggg tcgacctgca gaagcttaga tctattaccc
     3361 tgttatccct actcgagttc atgtgcagct ccataagcaa aaggggatga taagtttatc
     3421 accaccgact atttgcaaca gtgccgttga tcgtgctatg atcgactgat gtcatcagcg
     3481 gtggagtgca atgtcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     3541 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     3601 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     3661 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     3721 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     3781 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     3841 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     3901 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     3961 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     4021 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     4081 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     4141 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     4201 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     4261 gagatcacca aggtagtcgg caaataagat gccgctcgcc agtcgattgg ctgagctcat
     4321 aagttcctat tccgaagttc cgcgaacgcg taaaggatct aggtgaagat cctttttgat
     4381 aatctcatga ccaaaatccc ttaacgtcac acaacatagc cacggagttt tcgttccact
     4441 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4501 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4561 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4621 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4681 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4741 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4801 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4861 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4921 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4981 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5041 cgtcaggggg gcggagc
//
