LOCUS       sequence-332112-        6139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries fucO and aldA under the control of P_BAD 
            promoter, which is used for study about Improving ethylene glycol 
            utilization in Escherichia coli fermentation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Panda S, Fung V, Zhou J, Liang H, Zhou K
  TITLE     Improving ethylene glycol utilization in Escherichia coli 
            fermentation
  JOURNAL   Biochemical Engineering Journal
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochemical
            Engineering Journal"
COMMENT     Sequence Label: pEG01
FEATURES             Location/Qualifiers
     source          1..6139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..292
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     215..234
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             318..340
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(3028..3046)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3042..3089
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             3370..4161
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4354..4942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5096..5113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(5242..6120)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac
       61 tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc
      121 gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc
      181 cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga
      241 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt
      301 tttgggctag aaataatttt gtttaacttt aagaaggaga tatacatatg gctaacagaa
      361 tgattctgaa cgaaacggca tggtttggtc ggggtgctgt tggggcttta accgatgagg
      421 tgaaacgccg tggttatcag aaggcgctga tcgtcaccga taaaacgctg gtgcaatgcg
      481 gcgtggtggc gaaagtgacc gataagatgg atgctgcagg gctggcatgg gcgatttacg
      541 acggcgtagt gcccaaccca acaattactg tcgtcaaaga agggctcggt gtattccaga
      601 atagcggcgc ggattacctg atcgctattg gtggtggttc tccacaggat acttgtaaag
      661 cgattggcat tatcagcaac aacccggagt ttgccgatgt gcgtagcctg gaagggcttt
      721 ccccgaccaa taaacccagt gtaccgattc tggcaattcc taccacagca ggtactgcgg
      781 cagaagtgac cattaactac gtgatcactg acgaagagaa acggcgcaag tttgtttgcg
      841 ttgatccgca tgatatcccg caggtggcgt ttattgacgc tgacatgatg gatggtatgc
      901 ctccagcgct gaaagctgcg acgggtgtcg atgcgctcac tcatgctatt gaggggtata
      961 ttacccgtgg cgcgtgggcg ctaaccgatg cactgcacat taaagcgatt gaaatcattg
     1021 ctggggcgct gcgaggatcg gttgctggtg ataaggatgc cggagaagaa atggcgctcg
     1081 ggcagtatgt tgcgggtatg ggcttctcga atgttgggtt agggttggtg catggtatgg
     1141 cgcatccact gggcgcgttt tataacactc cacacggtgt tgcgaacgcc atcctgttac
     1201 cgcatgtcat gcgttataac gctgacttta ccggtgagaa gtaccgcgat atcgcgcgcg
     1261 ttatgggcgt gaaagtggaa ggtatgagcc tggaagaggc gcgtaatgcc gctgttgaag
     1321 cggtgtttgc tctcaaccgt gatgtcggta ttccgccaca tttgcgtgat gttggtgtac
     1381 gcaaggaaga cattccggca ctggcgcagg cggcactgga tgatgtttgt accggtggca
     1441 acccgcgtga agcaacgctt gaggatattg tagagcttta ccataccgcc tggtaaccgt
     1501 tcatttatca caaaaggatt gttcgatgtc agtacccgtt caacatccta tgtatatcga
     1561 tggacagttt gttacctggc gtggagacgc atggattgat gtggtaaacc ctgctacaga
     1621 ggctgtcatt tcccgcatac ccgatggtca ggccgaggat gcccgtaagg caatcgatgc
     1681 agcagaacgt gcacaaccag aatgggaagc gttgcctgct attgaacgcg ccagttggtt
     1741 gcgcaaaatc tccgccggga tccgcgaacg cgccagtgaa atcagtgcgc tgattgttga
     1801 agaagggggc aagatccagc agctggctga agtcgaagtg gcttttactg ccgactatat
     1861 cgattacatg gcggagtggg cacggcgtta cgagggcgag attattcaaa gcgatcgtcc
     1921 aggagaaaat attcttttgt ttaaacgtgc gcttggtgtg actaccggca ttctgccgtg
     1981 gaacttcccg ttcttcctca ttgcccgcaa aatggctccc gctcttttga ccggtaatac
     2041 catcgtcatt aaacctagtg aatttacgcc aaacaatgcg attgcattcg ccaaaatcgt
     2101 cgatgaaata ggccttccgc gcggcgtgtt taaccttgta ctggggcgtg gtgaaaccgt
     2161 tgggcaagaa ctggcgggta acccaaaggt cgcaatggtc agtatgacag gcagcgtctc
     2221 tgcaggtgag aagatcatgg cgactgcggc gaaaaacatc accaaagtgt gtctggaatt
     2281 ggggggtaaa gcaccagcta tcgtaatgga cgatgccgat cttgaactgg cagtcaaagc
     2341 catcgttgat tcacgcgtca ttaatagtgg gcaagtgtgt aactgtgcag aacgtgttta
     2401 tgtacagaaa ggcatttatg atcagttcgt caatcggctg ggtgaagcga tgcaggcggt
     2461 tcaatttggt aaccccgctg aacgcaacga cattgcgatg gggccgttga ttaacgccgc
     2521 ggcgctggaa agggtcgagc aaaaagtggc gcgcgcagta gaagaagggg cgagagtggc
     2581 gttcggtggc aaagcggtag aggggaaagg atattattat ccgccgacat tgctgctgga
     2641 tgttcgccag gaaatgtcga ttatgcatga ggaaaccttt ggcccggtgc tgccagttgt
     2701 cgcatttgac acgctggaag atgctatctc aatggctaat gacagtgatt acggcctgac
     2761 ctcatcaatc tatacccaaa atctgaacgt cgcgatgaaa gccattaaag ggctgaagtt
     2821 tggtgaaact tacatcaacc gtgaaaactt cgaagctatg caaggcttcc acgccggatg
     2881 gcgtaaatcc ggtattggcg gcgcagatgg taaacatggc ttgcatgaat atctgcagac
     2941 ccaggtggtt tatttacagt cttgatgggc tgaagggttt aagggctgct aacaaagccc
     3001 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     3061 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatcccg
     3121 caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt
     3181 gccttccctc gactcacact tgggtcgacc tgcagaagct tagatctatt accctgttat
     3241 ccctactcga gttcatgtgc agctccataa gcaaaagggg atgataagtt tatcaccacc
     3301 gactatttgc aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag
     3361 tgcaatgtca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     3421 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     3481 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     3541 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     3601 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     3661 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     3721 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     3781 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     3841 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     3901 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     3961 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     4021 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     4081 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     4141 accaaggtag tcggcaaata agatgccgct cgccagtcga ttggctgagc tcataagttc
     4201 ctattccgaa gttccgcgaa cgcgtaaagg atctaggtga agatcctttt tgataatctc
     4261 atgaccaaaa tcccttaacg ttcacacaac atagccacgg gagttttcgt tccactgagc
     4321 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4381 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4441 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4501 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4561 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4621 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4681 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4741 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4801 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtattt
     4861 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4921 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4981 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5041 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5101 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     5161 cggtatttca caccgcatat gctggatcct tcaaacagaa aggccatggg tcaagccgtc
     5221 aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc
     5281 acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa
     5341 atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt
     5401 gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat
     5461 ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc
     5521 gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc
     5581 gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag
     5641 taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc
     5701 ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg
     5761 aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg
     5821 cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg
     5881 atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc
     5941 ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc
     6001 cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc
     6061 caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat
     6121 acttttcata ctcccgcca
//
