LOCUS       sequence-332116-        5367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries fucO and aldA under the control of P_gyrA 
            promoter, which is used for study about Improving ethylene glycol 
            utilization in Escherichia coli fermentation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Panda S, Fung V, Zhou J, Liang H, Zhou K
  TITLE     Improving ethylene glycol utilization in Escherichia coli 
            fermentation
  JOURNAL   Biochemical Engineering Journal
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochemical
            Engineering Journal"
COMMENT     Sequence Label: pEG03
FEATURES             Location/Qualifiers
     source          1..5367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             208..230
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(3269..3928)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3843..3862
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3929..4031)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4442..4459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4576..5121)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4656..4675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgacggctt caaatttagc gatctcttcg tggtctacgt tatggttttc cggcgatttt
       61 tcggcattca ttggcacttc tactccgtaa ttggcaagac aaacgagtat atcaggcatt
      121 ggatgtgaat aaagcgtata ggtttacctc aaactgcgcg gctgtgttat aatttgcgac
      181 ctttgaatcc gggatactag aaataatttt gtttaacttt aagaaggaga tatacatatg
      241 gctaacagaa tgattctgaa cgaaacggca tggtttggtc ggggtgctgt tggggcttta
      301 accgatgagg tgaaacgccg tggttatcag aaggcgctga tcgtcaccga taaaacgctg
      361 gtgcaatgcg gcgtggtggc gaaagtgacc gataagatgg atgctgcagg gctggcatgg
      421 gcgatttacg acggcgtagt gcccaaccca acaattactg tcgtcaaaga agggctcggt
      481 gtattccaga atagcggcgc ggattacctg atcgctattg gtggtggttc tccacaggat
      541 acttgtaaag cgattggcat tatcagcaac aacccggagt ttgccgatgt gcgtagcctg
      601 gaagggcttt ccccgaccaa taaacccagt gtaccgattc tggcaattcc taccacagca
      661 ggtactgcgg cagaagtgac cattaactac gtgatcactg acgaagagaa acggcgcaag
      721 tttgtttgcg ttgatccgca tgatatcccg caggtggcgt ttattgacgc tgacatgatg
      781 gatggtatgc ctccagcgct gaaagctgcg acgggtgtcg atgcgctcac tcatgctatt
      841 gaggggtata ttacccgtgg cgcgtgggcg ctaaccgatg cactgcacat taaagcgatt
      901 gaaatcattg ctggggcgct gcgaggatcg gttgctggtg ataaggatgc cggagaagaa
      961 atggcgctcg ggcagtatgt tgcgggtatg ggcttctcga atgttgggtt agggttggtg
     1021 catggtatgg cgcatccact gggcgcgttt tataacactc cacacggtgt tgcgaacgcc
     1081 atcctgttac cgcatgtcat gcgttataac gctgacttta ccggtgagaa gtaccgcgat
     1141 atcgcgcgcg ttatgggcgt gaaagtggaa ggtatgagcc tggaagaggc gcgtaatgcc
     1201 gctgttgaag cggtgtttgc tctcaaccgt gatgtcggta ttccgccaca tttgcgtgat
     1261 gttggtgtac gcaaggaaga cattccggca ctggcgcagg cggcactgga tgatgtttgt
     1321 accggtggca acccgcgtga agcaacgctt gaggatattg tagagcttta ccataccgcc
     1381 tggtaaccgt tcatttatca caaaaggatt gttcgatgtc agtacccgtt caacatccta
     1441 tgtatatcga tggacagttt gttacctggc gtggagacgc atggattgat gtggtaaacc
     1501 ctgctacaga ggctgtcatt tcccgcatac ccgatggtca ggccgaggat gcccgtaagg
     1561 caatcgatgc agcagaacgt gcacaaccag aatgggaagc gttgcctgct attgaacgcg
     1621 ccagttggtt gcgcaaaatc tccgccggga tccgcgaacg cgccagtgaa atcagtgcgc
     1681 tgattgttga agaagggggc aagatccagc agctggctga agtcgaagtg gcttttactg
     1741 ccgactatat cgattacatg gcggagtggg cacggcgtta cgagggcgag attattcaaa
     1801 gcgatcgtcc aggagaaaat attcttttgt ttaaacgtgc gcttggtgtg actaccggca
     1861 ttctgccgtg gaacttcccg ttcttcctca ttgcccgcaa aatggctccc gctcttttga
     1921 ccggtaatac catcgtcatt aaacctagtg aatttacgcc aaacaatgcg attgcattcg
     1981 ccaaaatcgt cgatgaaata ggccttccgc gcggcgtgtt taaccttgta ctggggcgtg
     2041 gtgaaaccgt tgggcaagaa ctggcgggta acccaaaggt cgcaatggtc agtatgacag
     2101 gcagcgtctc tgcaggtgag aagatcatgg cgactgcggc gaaaaacatc accaaagtgt
     2161 gtctggaatt ggggggtaaa gcaccagcta tcgtaatgga cgatgccgat cttgaactgg
     2221 cagtcaaagc catcgttgat tcacgcgtca ttaatagtgg gcaagtgtgt aactgtgcag
     2281 aacgtgttta tgtacagaaa ggcatttatg atcagttcgt caatcggctg ggtgaagcga
     2341 tgcaggcggt tcaatttggt aaccccgctg aacgcaacga cattgcgatg gggccgttga
     2401 ttaacgccgc ggcgctggaa agggtcgagc aaaaagtggc gcgcgcagta gaagaagggg
     2461 cgagagtggc gttcggtggc aaagcggtag aggggaaagg atattattat ccgccgacat
     2521 tgctgctgga tgttcgccag gaaatgtcga ttatgcatga ggaaaccttt ggcccggtgc
     2581 tgccagttgt cgcatttgac acgctggaag atgctatctc aatggctaat gacagtgatt
     2641 acggcctgac ctcatcaatc tatacccaaa atctgaacgt cgcgatgaaa gccattaaag
     2701 ggctgaagtt tggtgaaact tacatcaacc gtgaaaactt cgaagctatg caaggcttcc
     2761 acgccggatg gcgtaaatcc ggtattggcg gcgcagatgg taaacatggc ttgcatgaat
     2821 atctgcagac ccaggtggtt tatttacagt cttgatgggc tgaagggttt aagcgaagac
     2881 gaacaataag gcctcccaaa tcggggggcc ttttttattg ataacaaaaa ggcaacacta
     2941 tgacatcgga aaacccgtta ctggcgctgc gagagaaaat cagcgcgctg gatgaaaaat
     3001 tattagcgtt actggcagag aggcgcgaat tcaaacagaa aggccatggg ctggcctcag
     3061 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     3121 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
     3181 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
     3241 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
     3301 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     3361 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     3421 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     3481 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     3541 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     3601 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     3661 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     3721 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     3781 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     3841 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     3901 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     3961 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     4021 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     4081 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     4141 aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
     4201 gctccagtgg cttctgtttc tatcagctgt ccctttcaca caacatagcc acgggtgttc
     4261 agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag
     4321 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     4381 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     4441 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     4501 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     4561 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     4621 ctcaaatcag tggtgacgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4681 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     4741 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     4801 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     4861 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     4921 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     4981 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     5041 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     5101 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt
     5161 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
     5221 taattctcat gtttgacagc ttatcaccca gtcctgctcg cttcgctact tggccatacc
     5281 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     5341 gtcttccctc gactcacact tggggag
//
