LOCUS       sequence-332140-        9495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLyGo cloning vector for a sequence of interest (LPMO) in K. 
            phaffii. Vector encoding the alfa-amylase signal peptide and the 
            LyGo cassette (SapI-ccdB-SapI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Hernandez-Rollan C, Falkenberg KB, Rennig M, Bertelsen AB, Ipsen JO,
            Brander S, Daley DO, Johansen KS, Norholm MHH
  TITLE     LyGo: A Platform for Rapid Screening of Lytic Polysaccharide 
            Monooxygenase Production.
  JOURNAL   ACS Synth Biol. 2021 Apr 16;10(4):897-906. doi: 
            10.1021/acssynbio.1c00034. Epub 2021 Apr 2.
  PUBMED    33797234
REFERENCE   2  (bases 1 to 9495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00034"; journalName: "ACS Synth Biol"; date: 
            "2021-04-16- 16"; volume: "10"; issue: "4"; pages: "897-906"
COMMENT     Sequence Label: pLyGo-Kp-2
FEATURES             Location/Qualifiers
     source          1..9495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..978
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     sig_peptide     990..1049
                     /codon_start=1
                     /product="signal sequence of alpha-amylase from Aspergillus
                     niger"
                     /label=alpha-amylase signal sequence
                     /translation="MVAWWSLFLYGLQVAAPALA"
     CDS             complement(1065..1370)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDKSWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1167..1186)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      1591..1837
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(2250..4784)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(5196..6011)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5196..6011)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5919..5938
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6003..6022
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(6175..6194)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    6389..7137
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(7173..7195)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7281..7421
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7336..7355
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7436..7453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7607..8195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7687..7706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8366..9157,9158..9226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8989..9008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9227..9331)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9399..9417
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacgcagatc gggaacactg aaaaataaca gttattattc gagatctaac atccaaagac
       61 gaaaggttga atgaaacctt tttgccatcc gacatccaca ggtccattct cacacataag
      121 tgccaaacgc aacaggaggg gatacactag cagcagaccg ttgcaaacgc aggacctcca
      181 ctcctcttct cctcaacacc cacttttgcc atcgaaaaac cagcccagtt attgggcttg
      241 attggagctc gctcattcca attccttcta ttaggctact aacaccatga ctttattagc
      301 ctgtctatcc tggcccccct ggcgaggttc atgtttgttt atttccgaat gcaacaagct
      361 ccgcattaca cccgaacatc actccagatg agggctttct gagtgtgggg tcaaatagtt
      421 tcatgttccc caaatggccc aaaactgaca gtttaaacgc tgtcttggaa cctaatatga
      481 caaaagcgtg atctcatcca agatgaacta agtttggttc gttgaaatgc taacggccag
      541 ttggtcaaaa agaaacttcc aaaagtcggc ataccgtttg tcttgtttgg tattgattga
      601 cgaatgctca aaaataatct cattaatgct tagcgcagtc tctctatcgc ttctgaaccc
      661 cggtgcacct gtgccgaaac gcaaatgggg aaacacccgc tttttggatg attatgcatt
      721 gtctccacat tgtatgcttc caagattctg gtgggaatac tgctgatagc ctaacgttca
      781 tgatcaaaat ttaactgttc taacccctac ttgacagcaa tatataaaca gaaggaagct
      841 gccctgtctt aaaccttttt ttttatcatc attattagct tactttcata attgcgactg
      901 gttccaattg acaagctttt gattttaacg acttttaacg acaacttgag aagatcaaaa
      961 aacaactaat tattcgaagg atccaaacga tggtcgcttg gtggtctttg tttctgtacg
     1021 gtcttcaggt cgctgcacct gctttggctc atcgaagagc ttaattatat tccccagaac
     1081 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
     1141 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gcttttatcc
     1201 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg
     1261 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
     1321 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat agcatacctc
     1381 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc
     1441 gcaaatacgc atactgttat ctggctttta gtagcgaaaa aaccccgccg aagcggggtt
     1501 ttttgcggct cttcgtaatt cgccttagat atgactgttc ctcagttcaa gttgggcact
     1561 tacgagaaga ccggtcttgc tagattctaa tcaagaggat gtcagaatgc catttgcctg
     1621 agagatgcag gcttcatttt tgatactttt ttatttgtaa cctatatagt ataggatttt
     1681 ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc tcgcagctga
     1741 tgaatatctt gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt tcttggtatt
     1801 tcccactcct cttcagagta cagaagatta agtgagacgt tcgtttgtgc aagcttatcg
     1861 ataagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta
     1921 tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca
     1981 taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca
     2041 tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac
     2101 ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac
     2161 ttggagccac tatcgactac gcgatcatgg cgatcacacc cgtcctgtgg atctatcgaa
     2221 tctaaatgta agttaaaatc tctaaataat taaataagtc ccagtttctc catacgaacc
     2281 ttaacagcat tgcggtgagc atctagacct tcaacagcag ccagatccat cactgcttgg
     2341 ccaatatgtt tcagtccctc aggagttacg tcttgtgaag tgatgaactt ctggaaggtt
     2401 gcagtgttaa ctccgctgta ttgacgggca tatccgtacg ttggcaaagt gtggttggta
     2461 ccggaggagt aatctccaca actctctgga gagtaggcac caacaaacac agatccagcg
     2521 tgttgtactt gatcaacata agaagaagca ttctcgattt gcaggatcaa gtgttcagga
     2581 gcgtactgat tggacatttc caaagcctgc tcgtaggttg caaccgatag ggttgtagag
     2641 tgtgcaatac acttgcgtac aatttcaacc cttggcaact gcacagcttg gttgtgaaca
     2701 gcatcttcaa ttctggcaag ctccttgtct gtcatatcga cagccaacag aatcacctgg
     2761 gaatcaatac catgttcagc ttgagacaga aggtctgagg caacgaaatc tggatcagcg
     2821 tatttatcag caataactag aacttcagaa ggcccagcag gcatgtcaat actacacagg
     2881 gctgatgtgt cattttgaac catcatcttg gcagcagtaa cgaactggtt tcctggacca
     2941 aatattttgt cacacttagg aacagtttct gttccgtaag ccatagcagc tactgcctgg
     3001 gcgcctcctg ctagcacgat acacttagca ccaaccttgt gggcaacgta gatgacttct
     3061 ggggtaaggg taccatcctt cttaggtgga gatgcaaaaa caatttcttt gcaaccagca
     3121 actttggcag gaacacccag catcagggaa gtggaaggca gaattgcggt tccaccagga
     3181 atatagaggc caactttctc aataggtctt gcaaaacgag agcagactac accagggcaa
     3241 gtctcaactt gcaacgtctc cgttagttga gcttcatgga atttcctgac gttatctata
     3301 gagagatcaa tggctctctt aacgttatct ggcaattgca taagttcctc tgggaaagga
     3361 gcttctaaca caggtgtctt caaagcgact ccatcaaact tggcagttag ttctaaaagg
     3421 gctttgtcac cattttgacg aacattgtcg acaattggtt tgactaattc cataatctgt
     3481 tccgttttct ggataggacg acgaagggca tcttcaattt cttgtgagga ggccttagaa
     3541 acgtcaattt tgcacaattc aatacgacct tcagaaggga cttctttagg tttggattct
     3601 tctttaggtt gttccttggt gtatcctggc ttggcatctc ctttccttct agtgaccttt
     3661 agggacttca tatccaggtt tctctccacc tcgtccaacg tcacaccgta cttggcacat
     3721 ctaactaatg caaaataaaa taagtcagca cattcccagg ctatatcttc cttggattta
     3781 gcttctgcaa gttcatcagc ttcctcccta attttagcgt tcaacaaaac ttcgtcgtca
     3841 aataaccgtt tggtataaga accttctgga gcattgctct tacgatccca caaggtggct
     3901 tccatggctc taagaccctt tgattggcca aaacaggaag tgcgttccaa gtgacagaaa
     3961 ccaacacctg tttgttcaac cacaaatttc aagcagtctc catcacaatc caattcgata
     4021 cccagcaact tttgagttgc tccagatgta gcacctttat accacaaacc gtgacgacga
     4081 gattggtaga ctccagtttg tgtccttata gcctccggaa tagacttttt ggacgagtac
     4141 accaggccca acgagtaatt agaagagtca gccaccaaag tagtgaatag accatcgggg
     4201 cggtcagtag tcaaagacgc caacaaaatt tcactgacag ggaacttttt gacatcttca
     4261 gaaagttcgt attcagtagt caattgccga gcatcaataa tggggattat accagaagca
     4321 acagtggaag tcacatctac caactttgcg gtctcagaaa aagcataaac agttctacta
     4381 ccgccattag tgaaactttt caaatcgccc agtggagaag aaaaaggcac agcgatacta
     4441 gcattagcgg gcaaggatgc aactttatca accagggtcc tatagataac cctagcgcct
     4501 gggatcatcc tttggacaac tctttctgcc aaatctaggt ccaaaatcac ttcattgata
     4561 ccattattgt acaacttgag caagttgtcg atcagctcct caaattggtc ctctgtaacg
     4621 gatgactcaa cttgcacatt aacttgaagc tcagtcgatt gagtgaactt gatcaggttg
     4681 tgcagctggt cagcagcata gggaaacacg gcttttccta ccaaactcaa ggaattatca
     4741 aactctgcaa cacttgcgta tgcaggtagc aagggaaatg tcatacttga agtcggacag
     4801 tgagtgtagt cttgagaaat tctgaagccg tatttttatt atcagtgagt cagtcatcag
     4861 gagatcctct acgccggacg catcgtggcc gacctgcagg gggggggggc gctgaggtct
     4921 gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca
     4981 gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt
     5041 gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt
     5101 caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg
     5161 ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
     5221 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     5281 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     5341 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     5401 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
     5461 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     5521 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     5581 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     5641 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     5701 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     5761 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     5821 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     5881 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     5941 tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt ttcccgttga
     6001 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     6061 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     6121 tccccccccc cctgcaggtc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     6181 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     6241 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     6301 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     6361 taatgcagga gtcgcataag ggagagcgtc gagtatctat gattggaagt atgggaatgg
     6421 tgatacccgc attcttcagt gtcttgaggt ctcctatcag attatgccca actaaagcaa
     6481 ccggaggagg agatttcatg gtaaatttct ctgacttttg gtcatcagta gactcgaact
     6541 gtgagactat ctcggttatg acagcagaaa tgtccttctt ggagacagta aatgaagtcc
     6601 caccaataaa gaaatccttg ttatcaggaa caaacttctt gtttcgaact ttttcggtgc
     6661 cttgaactat aaaatgtaga gtggatatgt cgggtaggaa tggagcgggc aaatgcttac
     6721 cttctggacc ttcaagaggt atgtagggtt tgtagatact gatgccaact tcagtgacaa
     6781 cgttgctatt tcgttcaaac cattccgaat ccagagaaat caaagttgtt tgtctactat
     6841 tgatccaagc cagtgcggtc ttgaaactga caatagtgtg ctcgtgtttt gaggtcatct
     6901 ttgtatgaat aaatctagtc tttgatctaa ataatcttga cgagccagac gataatacca
     6961 atctaaactc tttaaacgtt aaaggacaag tatgtctgcc tgtattaaac cccaaatcag
     7021 ctcgtagtct gatcctcatc aacttgaggg gcactatctt gttttagaga aatttgcgga
     7081 gatgcgatat cgagaaaaag gtacgctgat tttaaacgtg aaatttatct caagatctgc
     7141 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     7201 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7261 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     7321 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     7381 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcaacc gcttcctcgc
     7441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8041 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8401 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8461 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8521 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8581 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8641 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     8701 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8761 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8821 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8881 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8941 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     9001 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9061 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9121 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9181 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9241 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9301 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9361 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     9421 ttcgtcttca agaattaatt ctcatgtttg acagcttatc atcgataagc tgactcatgt
     9481 tggtattgtg aaata
//
