LOCUS       sequence-332171-         475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of 1xHA-tagged human androgen receptor 
            trancate: AR Ligand-bindind domain (LBD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Liu X, Han W, Gulla S, Simon NI, Gao Y, Cai C, Yang H, Zhang X, Liu 
            J, Balk SP, Chen S
  TITLE     Protein phosphatase 1 suppresses androgen receptor ubiquitylation 
            and degradation.
  JOURNAL   Oncotarget. 2016 Jan 12;7(2):1754-64. doi: 10.18632/oncotarget.6434.
  PUBMED    26636645
REFERENCE   2  (bases 1 to 475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget"; journalName: "Oncotarget"; date: "2016-01-12-
            12"; volume: "7"; issue: "2"; pages: "1754-64"
COMMENT     Sequence Label: HA-AR-LBD
FEATURES             Location/Qualifiers
     source          1..475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(251..277)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(314..333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(315..333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(378..429)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(407..427)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 catgtacgtt taatacatac gtattattca gctgtctctc tcccagttca ttgaggctag
       61 agagcaaggc tgcaaaggag tcgggctggt tgttgtcgtg tccagcacac actacacctg
      121 gctcaatggc ttccaggaca ttcagaaaga tgggctgaca ttcatagcct tcaatgtgtg
      181 acacaattcc agcacactgg cggccgttac tagtggatcc agctcgggta cctccggaat
      241 cgatgttaac ggcgtaatca gggacgtcgt aaggatagac catggccgca gctagccgcg
      301 gaagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta agcagtgggt
      361 tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg cctaccgccc
      421 atttgcgtca atgtttttgg agttgttacg acattttggc aagtcccgtt gagtt
//
