LOCUS       sequence-332205-        2122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Frangedakis E, Guzman-Chavez F, Rebmann M, Markel K, Yu Y, Perraki 
            A, Tse SW, Liu Y, Rever J, Sauret-Gueto S, Goffinet B, Schneider H, 
            Haseloff J
  TITLE     Construction of DNA Tools for Hyperexpression in Marchantia 
            Chloroplasts.
  JOURNAL   ACS Synth Biol. 2021 Jul 16;10(7):1651-1666. doi: 
            10.1021/acssynbio.0c00637. Epub 2021 Jun 7.
  PUBMED    34097383
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00637"; journalName: "ACS Synth Biol"; date: 
            "2021-07-16- 16"; volume: "10"; issue: "7"; pages: "1651-1666"
COMMENT     Sequence Label: PROM_Ntpsba_chlo
FEATURES             Location/Qualifiers
     source          1..2122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     479..498
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     726..744
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(748..791)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      889..946
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(970..987)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1141..1729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1221..1240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1912..2006)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2027..2122,1..564))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
       61 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      121 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      181 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      241 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      301 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
      361 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      421 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      481 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      541 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
      601 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
      661 cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
      721 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
      781 aggatgattt ctggaattcg gtctctagta ttattcaaca gtataacatg acttatatac
      841 tcgtgtcaac caagagctcg aattggccgc tctccagaga ccctgcagtc cggcaaaaaa
      901 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
      961 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1021 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1141 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1321 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1561 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1861 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc
     1921 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1981 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     2041 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     2101 aaacggcatg atgaacctga at
//
