LOCUS       sequence-332206-       11049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A 
            fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Ciurkot K, Gorochowski TE, Roubos JA, Verwaal R
  TITLE     Efficient multiplexed gene regulation in Saccharomyces cerevisiae 
            using dCas12a.
  JOURNAL   Nucleic Acids Res. 2021 Jul 1. pii: 6312738. doi: 
            10.1093/nar/gkab529.
  PUBMED    34197613
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 1. pii: 6312738. doi: 10.1093/nar/gkab529."
COMMENT     Sequence Label: pCSN075
FEATURES             Location/Qualifiers
     source          1..11049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        207..620
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4330..4350
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4822..4855
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            4901..6257
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4901..5244
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5245..6054
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5312..5331)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6060..6257
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    6305..6338
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6406..6426)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6406..6424)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6445..6461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6445..6461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6458..6480)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6469..6485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6493..6499,6500..6517,6518..6523))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6538..6559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6676..6693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6847..7435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6927..6946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7606..8397,8398..8466))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8229..8248
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8467..8571)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8608..9111
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9152..9170
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9208..9230)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9330..9349
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9370..9650
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9651..10325
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10428..10883)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10565..10586)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10777..10796
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11009..11031
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11023..11040
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11024..11040
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accttggctg ataatagcgt ataaacaatg
       61 catactttgt acgttcaaaa tacaatgcag tagatatatt tatgcatatt acatataata
      121 catatcacat aggaagcaac aggcgcgttg gacttttaat tttcgaggac cgcgaatcct
      181 tacatcacac ccaatccccc acaagtgatc ccccacacac catagcttca aaatgtttct
      241 actccttttt tactcttcca gattttctcg gactccgcgc atcgccgtac cacttcaaaa
      301 cacccaagca cagcatacta aatttcccct ctttcttcct ctagggtgtc gttaattacc
      361 cgtactaaag gtttggaaaa gaaaaaagac accgcctcgt ttctttttct tcgtcgaaaa
      421 aggcaataaa aatttttatc acgtttcttt ttcttgaaaa tttttttttt tgattttttt
      481 ctctttcgat gacctcccat tgatatttaa gttaataaac ggtcttcaat ttctcaagtt
      541 tcagtttcat ttttcttgtt ctattacaac tttttttact tcttgctcat tagaaagaaa
      601 gcatagcaat ctaatctaag ttttaattac aaaatgtcta agttggaaaa attcaccaac
      661 tgttactctt tgtctaagac tttgagattc aaggccatcc cagttggtaa gacccaagaa
      721 aacatcgaca acaagagact attagttgaa gatgaaaaga gagctgaaga ctacaagggt
      781 gtcaagaaat tgttggacag atactacttg tcttttatca acgacgtttt gcattccatc
      841 aagctaaaga acttgaataa ctacatctct ttgttcagaa agaagactag aactgaaaag
      901 gaaaataagg aattggaaaa cttggaaatc aacttgagaa aggaaattgc taaggctttc
      961 aagggtaatg aaggttacaa gtctttattc aagaaagaca tcattgaaac cattttgcca
     1021 gaatttttgg atgataagga tgaaattgct ttggttaact ctttcaacgg tttcaccact
     1081 gctttcactg gtttcttcga caacagagaa aacatgttct ccgaggaagc taaatccact
     1141 tctattgctt tcagatgtat caacgaaaac ttgacccgtt acatctctaa catggacatt
     1201 tttgaaaagg tcgacgccat ctttgacaag cacgaagtcc aagaaatcaa ggaaaagatc
     1261 ttaaactccg actacgatgt cgaagatttc ttcgaaggtg aattcttcaa ctttgtttta
     1321 acccaagaag gtatcgatgt ctacaacgcc attatcggtg gttttgtcac tgaatctggt
     1381 gaaaagatca agggtttgaa cgaatacatt aacttgtaca accaaaagac caaacaaaaa
     1441 ttgccaaagt tcaagccatt gtacaagcaa gttttgtctg acagagaatc tttgtctttt
     1501 tacggtgaag ggtacacctc tgacgaagaa gtcttggaag tcttcagaaa cactttgaac
     1561 aagaactctg aaatcttctc ctccatcaag aagttagaaa agttgttcaa gaacttcgat
     1621 gaatactctt ctgctggtat cttcgttaag aacggtccag ccatctctac catttctaag
     1681 gatatctttg gtgaatggaa cgtcattaga gacaaatgga acgctgaata cgatgacatc
     1741 catttgaaga aaaaggctgt tgtcaccgaa aagtacgaag acgacagaag aaaatccttc
     1801 aagaagatcg gttccttctc cttggaacaa ttacaagaat acgccgatgc cgatttgtcc
     1861 gttgtcgaaa aattgaagga aattattatt caaaaggttg atgaaattta caaagtttac
     1921 ggttcctctg aaaagttatt cgatgctgat ttcgtcttgg aaaagtcttt gaagaagaac
     1981 gacgctgttg tcgctatcat gaaggacttg ttggactctg tcaaatcttt cgaaaactat
     2041 atcaaggcct tcttcggtga aggtaaggaa actaacagag atgaatcctt ctacggtgac
     2101 tttgtcttgg cttacgatat tttgttgaag gttgaccaca tctacgatgc catcagaaac
     2161 tacgttactc aaaagccata ctctaaggac aaattcaagt tgtacttcca aaacccacaa
     2221 ttcatgggtg gttgggataa ggacaaggaa actgactaca gagctaccat tttgagatac
     2281 ggttccaagt actacttggc catcatggac aagaagtacg ccaagtgttt gcaaaagatt
     2341 gacaaggacg atgtcaacgg taactacgaa aagattaact acaagttgtt gccaggtcca
     2401 aacaagatgt tgccaaaggt tttcttctcc aaaaagtgga tggcttacta caacccatct
     2461 gaagacatcc aaaagatcta caagaacggt actttcaaaa agggtgacat gttcaactta
     2521 aacgactgtc acaagttgat cgacttcttc aaggactcca tctctagata cccaaaatgg
     2581 tccaacgctt acgatttcaa cttctctgaa actgaaaaat acaaggatat tgctggtttc
     2641 taccgtgaag tcgaggaaca aggttataag gtttctttcg aatccgcttc taagaaagaa
     2701 gttgacaaat tagtcgaaga aggtaagttg tacatgttcc aaatctacaa caaagatttc
     2761 tccgacaagt ctcacggtac tccaaacttg cacaccatgt acttcaagtt gctattcgat
     2821 gaaaacaacc acggtcaaat cagattgtct ggtggtgctg aattgttcat gagacgtgct
     2881 tctctaaaga aggaagaatt agtcgtccac ccagctaact ctccaattgc caacaagaac
     2941 ccagacaacc ctaagaagac caccactttg tcctacgacg tttacaagga caagagattc
     3001 tccgaagacc aatacgaatt gcacattcca attgctatca acaagtgtcc aaagaacatc
     3061 ttcaagatca acactgaagt cagagttttg ttaaagcacg atgacaaccc ttacgttatt
     3121 ggtatcgacc gtggtgaaag aaatttgttg tacattgttg ttgttgacgg taagggtaac
     3181 atcgttgaac aatactcctt gaacgaaatc atcaacaact tcaacggtat tagaatcaag
     3241 actgattacc actctttgtt ggataagaag gaaaaggaac gttttgaagc tcgtcaaaac
     3301 tggacctcta ttgaaaacat caaagaattg aaggctggtt acatcagtca agttgtccac
     3361 aagatctgtg aattggtcga gaagtacgat gccgttattg ccttggcaga tttgaactct
     3421 ggttttaaga actctcgtgt caaggttgaa aagcaagtct accaaaagtt cgaaaagatg
     3481 ttaatcgaca aattgaacta catggttgac aagaaatcca acccatgtgc taccggtggt
     3541 gctttgaaag gttaccaaat caccaacaaa ttcgaatctt tcaaatctat gtccactcaa
     3601 aacgggttca tcttctacat tccagcttgg ttgacctcca agatcgaccc atctaccggt
     3661 ttcgttaact tgttgaagac caagtacact tccattgctg attccaagaa gttcatctct
     3721 tctttcgaca gaatcatgta cgttccagaa gaagacttgt tcgaattcgc cttggactat
     3781 aagaacttct ccagaaccga tgctgactac attaagaaat ggaaattgta ctcctacggt
     3841 aacagaatca gaattttcag aaacccaaag aaaaacaacg ttttcgattg ggaagaagtt
     3901 tgtttgactt ctgcctacaa ggaattattc aacaaatacg gtatcaacta ccaacaaggt
     3961 gatatcagag ctttgttgtg tgaacaatct gacaaggctt tctactcttc cttcatggct
     4021 ttgatgtcct tgatgttgca aatgagaaac tccatcactg gtagaactga tgtcgacttc
     4081 ctcatttctc cagttaagaa ttctgacggt attttctacg actctagaaa ttacgaagct
     4141 caagaaaacg ctattttgcc aaagaacgct gatgctaacg gtgcttacaa tattgctaga
     4201 aaggttttgt gggctatcgg tcaattcaag aaggctgaag acgaaaagct agacaaggtc
     4261 aagattgcta tttctaacaa ggaatggttg gaatacgctc aaacctccgt caagcactcc
     4321 agagctgatc caaagaagaa gagaaaggta atggaacgtg tcagaatgat caacgttcaa
     4381 agattgttgg aagctgctga attcttggaa agaagagaaa gagaatgtga acacggttac
     4441 gcctcctctt tcccatccat gccatctcca agataaagga gttaaaggca aagttttctt
     4501 ttctagagcc gttcccacaa ataattatac gtatatgctt cttttcgttt actatatatc
     4561 tatatttaca agcctttatt cactgatgca atttgtttcc aaatactttt ttggagatct
     4621 cataactaga tatcatgatg gcgcaacttg gcgctatctt aattactctg gctgccaggc
     4681 ccgtgtagag ggccgcaaga ccttctgtac gccatatagt ctctaagaac ttgaacaagt
     4741 ttctagacct attgccgcct ttcggatcgc tattgttgcg gccgccagct gaagcttcgt
     4801 acgctgcagg tcgacgaatt ctaccgttcg tataatgtat gctatacgaa gttatagatc
     4861 tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
     4921 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
     4981 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
     5041 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
     5101 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
     5161 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
     5221 catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga
     5281 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     5341 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg
     5401 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     5461 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     5521 tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga agaatatcct
     5581 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     5641 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     5701 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     5761 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
     5821 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     5881 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     5941 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     6001 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagta
     6061 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
     6121 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     6181 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     6241 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg tcgaaaacga
     6301 gctcataact tcgtataatg tatgctatac gaacggtaga attcgatatc agatccacta
     6361 gtggcctatg cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt
     6421 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7261 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7921 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8221 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8581 ccgaaaagtg ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat
     8641 tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat
     8701 agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt
     8761 tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga
     8821 agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc
     8881 tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat
     8941 tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt
     9001 actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat
     9061 tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtctaaga
     9121 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     9181 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     9241 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     9301 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     9361 ccatagatca cgacattact atatatataa tataggaagc atttaataga acagcatcgt
     9421 aatatatgtg tactttgcag ttatgacgcc agatggcagt agtggaagat attctttatt
     9481 gaaaaatagc ttgtcacctt acgtacaatc ttgatccgga gcttttcttt ttttgccgat
     9541 taagaattaa ttcggtcgaa aaaagaaaag gagagggcca agagggaggg cattggtgac
     9601 tattgagcac gtgagtatac gtgattaagc acacaaaggc agcttggagt atgtctgtta
     9661 ttaatttcac aggtagttct ggtccattgg tgaaagtttg cggcttgcag agcacagagg
     9721 ccgcagaatg tgctctagat tccgatgctg acttgctggg tattatatgt gtgcccaata
     9781 gaaagagaac aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata
     9841 aaaatagttc aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg
     9901 atgttttggc tctggtcaat gattacggca ttgatatcgt ccaactgcat ggagatgagt
     9961 cgtggcaaga ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa
    10021 aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt
    10081 ttgattcaga agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg
    10141 ttggaaggca agagagcccc gaaagcttac attttatgtt agctggtgga ctgacgccag
    10201 aaaatgttgg tgatgcgctt agattaaatg gcgttattgg tgttgatgta agcggaggtg
    10261 tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga
    10321 aataggttat tactgagtag tatttattta agtattgttt gtgcacttgc cgatctatgc
    10381 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt
    10441 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
    10501 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
    10561 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
    10621 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
    10681 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
    10741 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
    10801 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
    10861 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
    10921 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    10981 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    11041 gagcgcgcg
//
