LOCUS       sequence-332207-       11104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A 
            fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Ciurkot K, Gorochowski TE, Roubos JA, Verwaal R
  TITLE     Efficient multiplexed gene regulation in Saccharomyces cerevisiae 
            using dCas12a.
  JOURNAL   Nucleic Acids Res. 2021 Jul 1. pii: 6312738. doi: 
            10.1093/nar/gkab529.
  PUBMED    34197613
REFERENCE   2  (bases 1 to 11104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 1. pii: 6312738. doi: 10.1093/nar/gkab529."
COMMENT     Sequence Label: pCSN071
FEATURES             Location/Qualifiers
     source          1..11104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        207..621
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4508..4528
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4877..4910
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            4956..6312
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4956..5299
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5300..6109
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5367..5386)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6115..6312
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    6360..6393
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6461..6481)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6461..6479)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6500..6516)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6500..6516)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6513..6535)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6524..6540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6548..6554,6555..6572,6573..6578))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6593..6614
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6731..6748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6902..7490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6982..7001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7661..8452,8453..8521))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8284..8303
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8522..8626)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8663..9166
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9207..9225
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9263..9285)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9385..9404
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9425..9705
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9706..10380
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10483..10938)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10620..10641)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10832..10851
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11064..11086
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11078..11095
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11079..11095
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accttggctg ataatagcgt ataaacaatg
       61 catactttgt acgttcaaaa tacaatgcag tagatatatt tatgcatatt acatataata
      121 catatcacat aggaagcaac aggcgcgttg gacttttaat tttcgaggac cgcgaatcct
      181 tacatcacac ccaatccccc acaagtgatc ccccacacac catagcttca aaatgtttct
      241 actccttttt tactcttcca gattttctcg gactccgcgc atcgccgtac cacttcaaaa
      301 cacccaagca cagcatacta aatttcccct ctttcttcct ctagggtgtc gttaattacc
      361 cgtactaaag gtttggaaaa gaaaaaagac accgcctcgt ttctttttct tcgtcgaaaa
      421 aggcaataaa aatttttatc acgtttcttt ttcttgaaaa tttttttttt ttgatttttt
      481 tctctttcga tgacctccca ttgatattta agttaataaa cggtcttcaa tttctcaagt
      541 ttcagtttca tttttcttgt tctattacaa ctttttttac ttcttgctca ttagaaagaa
      601 agcatagcaa tctaatctaa gttttaatta caaaatgtct aagttggaaa aattcaccaa
      661 ctgttactct ttgtctaaga ctttgagatt caaggccatc ccagttggta agacccaaga
      721 aaacatcgac aacaagagac tattagttga agatgaaaag agagctgaag actacaaggg
      781 tgtcaagaaa ttgttggaca gatactactt gtcttttatc aacgacgttt tgcattccat
      841 caagctaaag aacttgaata actacatctc tttgttcaga aagaagacta gaactgaaaa
      901 ggaaaataag gaattggaaa acttggaaat caacttgaga aaggaaattg ctaaggcttt
      961 caagggtaat gaaggttaca agtctttatt caagaaagac atcattgaaa ccattttgcc
     1021 agaatttttg gatgataagg atgaaattgc tttggttaac tctttcaacg gtttcaccac
     1081 tgctttcact ggtttcttcg acaacagaga aaacatgttc tccgaggaag ctaaatccac
     1141 ttctattgct ttcagatgta tcaacgaaaa cttgacccgt tacatctcta acatggacat
     1201 ttttgaaaag gtcgacgcca tctttgacaa gcacgaagtc caagaaatca aggaaaagat
     1261 cttaaactcc gactacgatg tcgaagattt cttcgaaggt gaattcttca actttgtttt
     1321 aacccaagaa ggtatcgatg tctacaacgc cattatcggt ggttttgtca ctgaatctgg
     1381 tgaaaagatc aagggtttga acgaatacat taacttgtac aaccaaaaga ccaaacaaaa
     1441 attgccaaag ttcaagccat tgtacaagca agttttgtct gacagagaat ctttgtcttt
     1501 ttacggtgaa gggtacacct ctgacgaaga agtcttggaa gtcttcagaa acactttgaa
     1561 caagaactct gaaatcttct cctccatcaa gaagttagaa aagttgttca agaacttcga
     1621 tgaatactct tctgctggta tcttcgttaa gaacggtcca gccatctcta ccatttctaa
     1681 ggatatcttt ggtgaatgga acgtcattag agacaaatgg aacgctgaat acgatgacat
     1741 ccatttgaag aaaaaggctg ttgtcaccga aaagtacgaa gacgacagaa gaaaatcctt
     1801 caagaagatc ggttccttct ccttggaaca attacaagaa tacgccgatg ccgatttgtc
     1861 cgttgtcgaa aaattgaagg aaattattat tcaaaaggtt gatgaaattt acaaagttta
     1921 cggttcctct gaaaagttat tcgatgctga tttcgtcttg gaaaagtctt tgaagaagaa
     1981 cgacgctgtt gtcgctatca tgaaggactt gttggactct gtcaaatctt tcgaaaacta
     2041 tatcaaggcc ttcttcggtg aaggtaagga aactaacaga gatgaatcct tctacggtga
     2101 ctttgtcttg gcttacgata ttttgttgaa ggttgaccac atctacgatg ccatcagaaa
     2161 ctacgttact caaaagccat actctaagga caaattcaag ttgtacttcc aaaacccaca
     2221 attcatgggt ggttgggata aggacaagga aactgactac agagctacca ttttgagata
     2281 cggttccaag tactacttgg ccatcatgga caagaagtac gccaagtgtt tgcaaaagat
     2341 tgacaaggac gatgtcaacg gtaactacga aaagattaac tacaagttgt tgccaggtcc
     2401 aaacaagatg ttgccaaagg ttttcttctc caaaaagtgg atggcttact acaacccatc
     2461 tgaagacatc caaaagatct acaagaacgg tactttcaaa aagggtgaca tgttcaactt
     2521 aaacgactgt cacaagttga tcgacttctt caaggactcc atctctagat acccaaaatg
     2581 gtccaacgct tacgatttca acttctctga aactgaaaaa tacaaggata ttgctggttt
     2641 ctaccgtgaa gtcgaggaac aaggttataa ggtttctttc gaatccgctt ctaagaaaga
     2701 agttgacaaa ttagtcgaag aaggtaagtt gtacatgttc caaatctaca acaaagattt
     2761 ctccgacaag tctcacggta ctccaaactt gcacaccatg tacttcaagt tgctattcga
     2821 tgaaaacaac cacggtcaaa tcagattgtc tggtggtgct gaattgttca tgagacgtgc
     2881 ttctctaaag aaggaagaat tagtcgtcca cccagctaac tctccaattg ccaacaagaa
     2941 cccagacaac cctaagaaga ccaccacttt gtcctacgac gtttacaagg acaagagatt
     3001 ctccgaagac caatacgaat tgcacattcc aattgctatc aacaagtgtc caaagaacat
     3061 cttcaagatc aacactgaag tcagagtttt gttaaagcac gatgacaacc cttacgttat
     3121 tggtatcgac cgtggtgaaa gaaatttgtt gtacattgtt gttgttgacg gtaagggtaa
     3181 catcgttgaa caatactcct tgaacgaaat catcaacaac ttcaacggta ttagaatcaa
     3241 gactgattac cactctttgt tggataagaa ggaaaaggaa cgttttgaag ctcgtcaaaa
     3301 ctggacctct attgaaaaca tcaaagaatt gaaggctggt tacatcagtc aagttgtcca
     3361 caagatctgt gaattggtcg agaagtacga tgccgttatt gccttggcag atttgaactc
     3421 tggttttaag aactctcgtg tcaaggttga aaagcaagtc taccaaaagt tcgaaaagat
     3481 gttaatcgac aaattgaact acatggttga caagaaatcc aacccatgtg ctaccggtgg
     3541 tgctttgaaa ggttaccaaa tcaccaacaa attcgaatct ttcaaatcta tgtccactca
     3601 aaacgggttc atcttctaca ttccagcttg gttgacctcc aagatcgacc catctaccgg
     3661 tttcgttaac ttgttgaaga ccaagtacac ttccattgct gattccaaga agttcatctc
     3721 ttctttcgac agaatcatgt acgttccaga agaagacttg ttcgaattcg ccttggacta
     3781 taagaacttc tccagaaccg atgctgacta cattaagaaa tggaaattgt actcctacgg
     3841 taacagaatc agaattttca gaaacccaaa gaaaaacaac gttttcgatt gggaagaagt
     3901 ttgtttgact tctgcctaca aggaattatt caacaaatac ggtatcaact accaacaagg
     3961 tgatatcaga gctttgttgt gtgaacaatc tgacaaggct ttctactctt ccttcatggc
     4021 tttgatgtcc ttgatgttgc aaatgagaaa ctccatcact ggtagaactg atgtcgactt
     4081 cctcatttct ccagttaaga attctgacgg tattttctac gactctagaa attacgaagc
     4141 tcaagaaaac gctattttgc caaagaacgc tgatgctaac ggtgcttaca atattgctag
     4201 aaaggttttg tgggctatcg gtcaattcaa gaaggctgaa gacgaaaagc tagacaaggt
     4261 caagattgct atttctaaca aggaatggtt ggaatacgct caaacctccg tcaagcacgg
     4321 ttcttccaag ttgggtggtt ctggtggttc catggaacgt gtcagaatga tcaacgttca
     4381 aagattgttg gaagctgctg aattcttgga aagaagagaa agagaatgtg aacacggtta
     4441 cgcctcctct ttcccatcca tgccatctcc aagaggtagt tccaagcttg gcggcagcgg
     4501 cggcagccca aagaagaaga gaaaggtata aaggagttaa aggcaaagtt ttcttttcta
     4561 gagccgttcc cacaaataat tatacgtata tgcttctttt cgtttactat atatctatat
     4621 ttacaagcct ttattcactg atgcaatttg tttccaaata cttttttgga gatctcataa
     4681 ctagatatca tgatggcgca acttggcgct atcttaatta ctctggctgc caggcccgtg
     4741 tagagggccg caagaccttc tgtacgccat atagtctcta agaacttgaa caagtttcta
     4801 gacctattgc cgcctttcgg atcgctattg ttgcggccgc cagctgaagc ttcgtacgct
     4861 gcaggtcgac gaattctacc gttcgtataa tgtatgctat acgaagttat agatctgttt
     4921 agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc
     4981 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
     5041 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
     5101 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
     5161 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
     5221 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
     5281 catccgaaca taaacaacca tgggtaagga aaagactcac gtttcgaggc cgcgattaaa
     5341 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     5401 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     5461 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     5521 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     5581 actcaccact gcgatccccg gcaaaacagc attccaggta ttagaagaat atcctgattc
     5641 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     5701 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     5761 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     5821 acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca
     5881 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     5941 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     6001 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     6061 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagtactgac
     6121 aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt
     6181 gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc
     6241 ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg
     6301 gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa aacgagctca
     6361 taacttcgta taatgtatgc tatacgaacg gtagaattcg atatcagatc cactagtggc
     6421 ctatgcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg
     6481 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6541 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6601 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6661 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6721 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6781 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6841 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6901 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6961 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7021 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7081 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7141 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7201 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7261 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7321 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7381 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7441 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7501 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7561 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7621 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7681 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7741 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7801 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7861 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7921 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7981 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8041 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8101 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8161 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8221 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8281 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8341 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8401 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8461 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8521 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8581 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8641 aagtgccacc tgggtccttt tcatcacgtg ctataaaaat aattataatt taaatttttt
     8701 aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt
     8761 ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct
     8821 tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaagacc
     8881 acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt
     8941 aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt gaaatttttt
     9001 aaacctttgt ttattttttt ttcttcattc cgtaactctt ctaccttctt tatttacttt
     9061 ctaaaatcca aatacaaaac ataaaaataa ataaacacag agtaaattcc caaattattc
     9121 catcattaaa agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc taagaaacca
     9181 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     9241 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     9301 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     9361 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     9421 gatcacgaca ttactatata tataatatag gaagcattta atagaacagc atcgtaatat
     9481 atgtgtactt tgcagttatg acgccagatg gcagtagtgg aagatattct ttattgaaaa
     9541 atagcttgtc accttacgta caatcttgat ccggagcttt tctttttttg ccgattaaga
     9601 attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg
     9661 agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat
     9721 ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca
     9781 gaatgtgctc tagattccga tgctgacttg ctgggtatta tatgtgtgcc caatagaaag
     9841 agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat
     9901 agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa ggaggatgtt
     9961 ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga tgagtcgtgg
    10021 caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt tccaaaagac
    10081 tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc cttgtttgat
    10141 tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga ctgggttgga
    10201 aggcaagaga gccccgaaag cttacatttt atgttagctg gtggactgac gccagaaaat
    10261 gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag
    10321 acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag
    10381 gttattactg agtagtattt atttaagtat tgtttgtgca cttgccgatc tatgcggtgt
    10441 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata
    10501 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
    10561 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
    10621 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
    10681 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
    10741 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
    10801 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
    10861 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
    10921 cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    10981 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    11041 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg
    11101 cgcg
//
