LOCUS       sequence-33221-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI Flag Smad3 LC WT
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          100..232
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     215..234
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        277..295
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             313..336
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcggtagttt atcacagtta aattgctaac gcagtcagtg cttctgacac aacagtctcg
       61 aacttaagct gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga
      121 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc
      181 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac
      241 tcccagttca attacagctc ttaaggctag agtacttaat acgactcact ataggctagc
      301 ctcgagacca tggactataa ggatgacgat gacaaagaat tcacgcgtgg tacctctaga
      361 ccagttctac ctcctgtgtt ggtgccacgc cacacagaga tcccggccga gttcccccca
      421 ctggacgact acagccattc catccccgaa aacactaact tccccgcagg catcgagccc
      481 cagagcaata ttccagagac cccaccccct ggctacctga gtgaagatgg agaaaccagt
      541 gaccaccaga tgaaccacag catggacgca ggttctccaa acctatcccc gaatccgatg
      601 tccccagcac ataataactt ggacctgcag ccagttacct actgcgagcc ggccttctgg
      661 tgctccatct cctactacga gctgaaccag cgcgtcgggg agacattcca cgcctcgcag
      721 ccatccatga ctgtggatgg cttcaccgac ccctccaatt cggagcgctt ctgcctaggg
      781 ctgctctcca atgtcaacag gaatgcagca gtgga
//
