LOCUS       sequence-332213-       10766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_TRE3GS-->GssGFP-P2A-NLSIFP
FEATURES             Location/Qualifiers
     source          1..10766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..39)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(24..40)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             130..363
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(388..406)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(389..406)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(696..715)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(929..948)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1048..1070
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1108..1126)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1194..1298
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1299..1367,1368..2159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1517..1536)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2330..2918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2819..2838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3072..3089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3159..3179)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3164..3493
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3344..3479
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4627..4692
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4822..4842
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5267..5401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5304..5323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5358..5377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5569..6202
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6249..6374
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6871..7104
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7289..7333
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7618..7735
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7814..8181
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    7822..7840
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7858..7876
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7894..7912
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7930..7948
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7966..7984
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8002..8020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8038..8056
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      8220..8229
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8277..8279,8280..8990)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             9006..9062
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9075..9095
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9099..9119
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9132..10094
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="ARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADG
                     HSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLDW
                     PAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQTV
                     RELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLRL
                     TADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGGQ
                     LWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     misc_feature    join(10198..10766,1..20)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10251..10271)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10669..10680)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg
       61 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
      121 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
      181 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      241 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      301 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      361 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
      421 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
      481 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
      541 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
      601 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
      661 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
      721 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
      781 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
      841 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
      901 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
      961 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     1021 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     1081 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     1141 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1201 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1261 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1321 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1381 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1441 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1501 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1561 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1621 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1681 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1741 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1801 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1861 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1921 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1981 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2041 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2101 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2161 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2221 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2281 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2341 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2401 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2461 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2521 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2581 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2641 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2701 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2761 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2821 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2881 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2941 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3001 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3061 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3121 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     3181 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3241 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3301 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3361 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3421 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3481 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     3541 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     3601 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     3661 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     3721 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     3781 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     3841 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     3901 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     3961 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     4021 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     4081 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     4141 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     4201 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     4261 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     4321 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     4381 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     4441 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     4501 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     4561 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     4621 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     4681 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     4741 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     4801 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     4861 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     4921 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     4981 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     5041 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     5101 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     5161 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     5221 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     5281 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     5401 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     5461 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     5521 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
     5581 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     5641 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     5701 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     5761 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     5821 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     5881 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     5941 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     6001 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6061 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6121 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6181 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     6241 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     6301 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     6361 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     6421 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     6481 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     6541 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     6601 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     6661 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     6721 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     6781 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     6841 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     6901 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     6961 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     7021 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     7081 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     7141 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     7201 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     7261 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     7321 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     7381 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     7441 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     7501 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     7561 cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca tccacaattt
     7621 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     7681 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     7741 ttattacagg gacagcagag atccagtttg gatcgataag cttgatatcg aattcccgtg
     7801 gaattatcac ctcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc
     7861 ctatcagtga tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa
     7921 ggagtttact ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata
     7981 gagaacgtat ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc
     8041 ctatcagtga tagagaacgt ataagctttg cttatgtaaa ccagggcgcc tataaaagag
     8101 tgctgatttt ttgagtaaac ttcaattcca caacactttt gtcttatacc aactttccgt
     8161 accacttcct accctcgtaa agaattcccg tatacaccgg tgcaattctc acgcgtgccg
     8221 ccaccatggg cgttaaagtc cttttcgccc tgatctgcat tgccgtcgca gaggccgttt
     8281 ctaagggtga agaattgttc accggagttg tgcccatcct ggttgagctg gacggagacg
     8341 ttaacggaca caagttctca gtgagcggcg aaggagaggg cgacgctacg tacggtaaac
     8401 tgacgttgaa gttcatctgt acaacaggaa agctgccagt gccctggcct acactcgtga
     8461 ccaccctcac ctacggagtc cagtgcttct cccggtaccc cgaccacatg aaacagcacg
     8521 atttcttcaa gtccgcgatg cctgagggct atgttcagga acgcaccata ttcttcaagg
     8581 acgacggtaa ctataaaacc agggctgaag ttaagttcga gggggacacg ttggtaaata
     8641 gaattgagtt gaaaggaatc gatttcaaag aagacggcaa tattttgggc cacaagctgg
     8701 agtacaacta caacagccac aacgtgtata ttatggctga taagcagaag aacggcatca
     8761 aggtgaactt caaaatccga cacaatatcg aggatggctc tgtgcaactg gccgaccatt
     8821 accagcaaaa cacgcctatc ggcgacggtc cggttttgct gccagataac cactacctct
     8881 ccacgcaatc agccctctcc aaggatccta acgaaaaaag agaccatatg gttctgcttg
     8941 aattcgtgac ggctgctggg atcaccctgg gaatggatga gctgtataag gccagcggca
     9001 gcggcgccac aaacttctct ctgctaaagc aagcaggtga tgttgaagaa aaccccgggc
     9061 ctcatatggt ggatccaaaa aagaagagaa aggtagatcc aaagaagaaa aggaaggttg
     9121 gcagcggctc cgctcgggac cctcaacctt tcttccctcc tctgtatctc ggaggaccag
     9181 agatcactac tgagaactgc gagcgggaac ctatacatat accaggttct attcaacctc
     9241 atggagcact gctcacagcc gatggacatt ctggcgaggt gctgcaagtt tcactcaacg
     9301 cagccacttt cctgggccag gaaccaactg ttctgcgggg acagaccctg gcagccctgc
     9361 tgccagacca gtggcctgct ctgcagactg ccctgcctcc aggctgccag gacgccctgc
     9421 agtatcgcgc caccctggat tggcccgccg ccggacacct gagcctgacc gtgcataggg
     9481 tggccgagct gctgattctg gaattcgagc ctactgaggc atgggactct attggacccc
     9541 acgctctgag aaatgcaatg ttcgctctgg agagtgctcc taatctccgg gcactggctg
     9601 aagtcgcaac ccaaacagtc cgggaactgt caggtttcga ccgggtgatg ctgtacaagt
     9661 ttgcaccaga cgcaacagga gaggttattg ccgaagcaag gcgcgagggc atgcaggctt
     9721 acctcgggca taggtttccc gcatccacca cccctgcaca agctagggcc ctctacacaa
     9781 gacacctgct ccggctgacc gcagacacca gggctgcagc agtgcccctc gaccccgtgc
     9841 tgaatcccca gacaaatgct cctacacctc tgggcggagc tgtcctcaga gctacatccc
     9901 caatgcacat gcagtacctg aggaatatgg gagtgggctc ctccctgagc gtcagcgtcg
     9961 tggtcggcgg ccagctgtgg ggactgattg tctgccacca tcagacaccc tacgtgctgc
    10021 cacccgatct gcggaccacc ctggagtatc tggggaggct cctgtccctg caggtgcaga
    10081 ggaaagaagc ctgattaatt aaggccgcaa ctcccacctg caacatgcgt gactgactga
    10141 ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct tatcgataat
    10201 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
    10261 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
    10321 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
    10381 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
    10441 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
    10501 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
    10561 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
    10621 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
    10681 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
    10741 cttcgccctc agacgagtcg gatctc
//
