LOCUS       sequence-332216-       11137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A 
            fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Ciurkot K, Gorochowski TE, Roubos JA, Verwaal R
  TITLE     Efficient multiplexed gene regulation in Saccharomyces cerevisiae 
            using dCas12a.
  JOURNAL   Nucleic Acids Res. 2021 Jul 1. pii: 6312738. doi: 
            10.1093/nar/gkab529.
  PUBMED    34197613
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 1. pii: 6312738. doi: 10.1093/nar/gkab529."
COMMENT     Sequence Label: pCSN072
FEATURES             Location/Qualifiers
     source          1..11137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        207..621
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4331..4351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4541..4561
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4910..4943
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            4989..6345
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4989..5332
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5333..6142
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5400..5419)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6148..6345
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    6393..6426
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6494..6514)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6494..6512)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6533..6549)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6533..6549)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6546..6568)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6557..6573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6581..6587,6588..6605,6606..6611))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6626..6647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6764..6781)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6935..7523)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7015..7034)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7694..8485,8486..8554))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8317..8336
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8555..8659)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8696..9199
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9240..9258
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9296..9318)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9418..9437
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9458..9738
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9739..10413
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10516..10971)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10653..10674)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10865..10884
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11097..11119
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11111..11128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11112..11128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accttggctg ataatagcgt ataaacaatg
       61 catactttgt acgttcaaaa tacaatgcag tagatatatt tatgcatatt acatataata
      121 catatcacat aggaagcaac aggcgcgttg gacttttaat tttcgaggac cgcgaatcct
      181 tacatcacac ccaatccccc acaagtgatc ccccacacac catagcttca aaatgtttct
      241 actccttttt tactcttcca gattttctcg gactccgcgc atcgccgtac cacttcaaaa
      301 cacccaagca cagcatacta aatttcccct ctttcttcct ctagggtgtc gttaattacc
      361 cgtactaaag gtttggaaaa gaaaaaagac accgcctcgt ttctttttct tcgtcgaaaa
      421 aggcaataaa aatttttatc acgtttcttt ttcttgaaaa tttttttttt ttgatttttt
      481 tctctttcga tgacctccca ttgatattta agttaataaa cggtcttcaa tttctcaagt
      541 ttcagtttca tttttcttgt tctattacaa ctttttttac ttcttgctca ttagaaagaa
      601 agcatagcaa tctaatctaa gttttaatta caaaatgtct aagttggaaa aattcaccaa
      661 ctgttactct ttgtctaaga ctttgagatt caaggccatc ccagttggta agacccaaga
      721 aaacatcgac aacaagagac tattagttga agatgaaaag agagctgaag actacaaggg
      781 tgtcaagaaa ttgttggaca gatactactt gtcttttatc aacgacgttt tgcattccat
      841 caagctaaag aacttgaata actacatctc tttgttcaga aagaagacta gaactgaaaa
      901 ggaaaataag gaattggaaa acttggaaat caacttgaga aaggaaattg ctaaggcttt
      961 caagggtaat gaaggttaca agtctttatt caagaaagac atcattgaaa ccattttgcc
     1021 agaatttttg gatgataagg atgaaattgc tttggttaac tctttcaacg gtttcaccac
     1081 tgctttcact ggtttcttcg acaacagaga aaacatgttc tccgaggaag ctaaatccac
     1141 ttctattgct ttcagatgta tcaacgaaaa cttgacccgt tacatctcta acatggacat
     1201 ttttgaaaag gtcgacgcca tctttgacaa gcacgaagtc caagaaatca aggaaaagat
     1261 cttaaactcc gactacgatg tcgaagattt cttcgaaggt gaattcttca actttgtttt
     1321 aacccaagaa ggtatcgatg tctacaacgc cattatcggt ggttttgtca ctgaatctgg
     1381 tgaaaagatc aagggtttga acgaatacat taacttgtac aaccaaaaga ccaaacaaaa
     1441 attgccaaag ttcaagccat tgtacaagca agttttgtct gacagagaat ctttgtcttt
     1501 ttacggtgaa gggtacacct ctgacgaaga agtcttggaa gtcttcagaa acactttgaa
     1561 caagaactct gaaatcttct cctccatcaa gaagttagaa aagttgttca agaacttcga
     1621 tgaatactct tctgctggta tcttcgttaa gaacggtcca gccatctcta ccatttctaa
     1681 ggatatcttt ggtgaatgga acgtcattag agacaaatgg aacgctgaat acgatgacat
     1741 ccatttgaag aaaaaggctg ttgtcaccga aaagtacgaa gacgacagaa gaaaatcctt
     1801 caagaagatc ggttccttct ccttggaaca attacaagaa tacgccgatg ccgatttgtc
     1861 cgttgtcgaa aaattgaagg aaattattat tcaaaaggtt gatgaaattt acaaagttta
     1921 cggttcctct gaaaagttat tcgatgctga tttcgtcttg gaaaagtctt tgaagaagaa
     1981 cgacgctgtt gtcgctatca tgaaggactt gttggactct gtcaaatctt tcgaaaacta
     2041 tatcaaggcc ttcttcggtg aaggtaagga aactaacaga gatgaatcct tctacggtga
     2101 ctttgtcttg gcttacgata ttttgttgaa ggttgaccac atctacgatg ccatcagaaa
     2161 ctacgttact caaaagccat actctaagga caaattcaag ttgtacttcc aaaacccaca
     2221 attcatgggt ggttgggata aggacaagga aactgactac agagctacca ttttgagata
     2281 cggttccaag tactacttgg ccatcatgga caagaagtac gccaagtgtt tgcaaaagat
     2341 tgacaaggac gatgtcaacg gtaactacga aaagattaac tacaagttgt tgccaggtcc
     2401 aaacaagatg ttgccaaagg ttttcttctc caaaaagtgg atggcttact acaacccatc
     2461 tgaagacatc caaaagatct acaagaacgg tactttcaaa aagggtgaca tgttcaactt
     2521 aaacgactgt cacaagttga tcgacttctt caaggactcc atctctagat acccaaaatg
     2581 gtccaacgct tacgatttca acttctctga aactgaaaaa tacaaggata ttgctggttt
     2641 ctaccgtgaa gtcgaggaac aaggttataa ggtttctttc gaatccgctt ctaagaaaga
     2701 agttgacaaa ttagtcgaag aaggtaagtt gtacatgttc caaatctaca acaaagattt
     2761 ctccgacaag tctcacggta ctccaaactt gcacaccatg tacttcaagt tgctattcga
     2821 tgaaaacaac cacggtcaaa tcagattgtc tggtggtgct gaattgttca tgagacgtgc
     2881 ttctctaaag aaggaagaat tagtcgtcca cccagctaac tctccaattg ccaacaagaa
     2941 cccagacaac cctaagaaga ccaccacttt gtcctacgac gtttacaagg acaagagatt
     3001 ctccgaagac caatacgaat tgcacattcc aattgctatc aacaagtgtc caaagaacat
     3061 cttcaagatc aacactgaag tcagagtttt gttaaagcac gatgacaacc cttacgttat
     3121 tggtatcgac cgtggtgaaa gaaatttgtt gtacattgtt gttgttgacg gtaagggtaa
     3181 catcgttgaa caatactcct tgaacgaaat catcaacaac ttcaacggta ttagaatcaa
     3241 gactgattac cactctttgt tggataagaa ggaaaaggaa cgttttgaag ctcgtcaaaa
     3301 ctggacctct attgaaaaca tcaaagaatt gaaggctggt tacatcagtc aagttgtcca
     3361 caagatctgt gaattggtcg agaagtacga tgccgttatt gccttggcag atttgaactc
     3421 tggttttaag aactctcgtg tcaaggttga aaagcaagtc taccaaaagt tcgaaaagat
     3481 gttaatcgac aaattgaact acatggttga caagaaatcc aacccatgtg ctaccggtgg
     3541 tgctttgaaa ggttaccaaa tcaccaacaa attcgaatct ttcaaatcta tgtccactca
     3601 aaacgggttc atcttctaca ttccagcttg gttgacctcc aagatcgacc catctaccgg
     3661 tttcgttaac ttgttgaaga ccaagtacac ttccattgct gattccaaga agttcatctc
     3721 ttctttcgac agaatcatgt acgttccaga agaagacttg ttcgaattcg ccttggacta
     3781 taagaacttc tccagaaccg atgctgacta cattaagaaa tggaaattgt actcctacgg
     3841 taacagaatc agaattttca gaaacccaaa gaaaaacaac gttttcgatt gggaagaagt
     3901 ttgtttgact tctgcctaca aggaattatt caacaaatac ggtatcaact accaacaagg
     3961 tgatatcaga gctttgttgt gtgaacaatc tgacaaggct ttctactctt ccttcatggc
     4021 tttgatgtcc ttgatgttgc aaatgagaaa ctccatcact ggtagaactg atgtcgactt
     4081 cctcatttct ccagttaaga attctgacgg tattttctac gactctagaa attacgaagc
     4141 tcaagaaaac gctattttgc caaagaacgc tgatgctaac ggtgcttaca atattgctag
     4201 aaaggttttg tgggctatcg gtcaattcaa gaaggctgaa gacgaaaagc tagacaaggt
     4261 caagattgct atttctaaca aggaatggtt ggaatacgct caaacctccg tcaagcactc
     4321 cagagctgat ccaaagaaga agagaaaggt aggttcttcc aagttgggtg gttctggtgg
     4381 ttccatggaa cgtgtcagaa tgatcaacgt tcaaagattg ttggaagctg ctgaattctt
     4441 ggaaagaaga gaaagagaat gtgaacacgg ttacgcctcc tctttcccat ccatgccatc
     4501 tccaagaggt agttccaagc ttggcggcag cggcggcagc cccaagaaaa agcgcaaggt
     4561 ataaaggagt taaaggcaaa gttttctttt ctagagccgt tcccacaaat aattatacgt
     4621 atatgcttct tttcgtttac tatatatcta tatttacaag cctttattca ctgatgcaat
     4681 ttgtttccaa atactttttt ggagatctca taactagata tcatgatggc gcaacttggc
     4741 gctatcttaa ttactctggc tgccaggccc gtgtagaggg ccgcaagacc ttctgtacgc
     4801 catatagtct ctaagaactt gaacaagttt ctagacctat tgccgccttt cggatcgcta
     4861 ttgttgcggc cgccagctga agcttcgtac gctgcaggtc gacgaattct accgttcgta
     4921 taatgtatgc tatacgaagt tatagatctg tttagcttgc ctcgtccccg ccgggtcacc
     4981 cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag
     5041 gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg
     5101 catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga
     5161 cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc
     5221 tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt
     5281 ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtaa
     5341 ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     5401 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
     5461 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     5521 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     5581 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggcaaaac
     5641 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     5701 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     5761 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     5821 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
     5881 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     5941 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     6001 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     6061 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     6121 gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt tttcaagaac
     6181 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
     6241 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
     6301 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
     6361 taacgccgcc atccagtgtc gaaaacgagc tcataacttc gtataatgta tgctatacga
     6421 acggtagaat tcgatatcag atccactagt ggcctatgcg gccgccaccg cggtggagct
     6481 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     6541 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6601 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6661 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6721 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6781 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6841 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6901 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6961 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7021 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7081 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7141 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7201 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7261 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7321 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7381 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7441 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7501 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7561 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7621 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7681 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7741 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7801 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7861 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7921 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7981 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8041 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8101 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8161 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8221 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8281 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8341 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8401 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8461 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8521 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8581 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8641 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac
     8701 gtgctataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga
     8761 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct
     8821 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg
     8881 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat
     8941 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat
     9001 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca
     9061 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa
     9121 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt
     9181 aagttacagg caagcgatcc gtctaagaaa ccattattat catgacatta acctataaaa
     9241 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     9301 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9361 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9421 catcagagca gattgtactg agagtgcacc atagatcacg acattactat atatataata
     9481 taggaagcat ttaatagaac agcatcgtaa tatatgtgta ctttgcagtt atgacgccag
     9541 atggcagtag tggaagatat tctttattga aaaatagctt gtcaccttac gtacaatctt
     9601 gatccggagc ttttcttttt ttgccgatta agaattaatt cggtcgaaaa aagaaaagga
     9661 gagggccaag agggagggca ttggtgacta ttgagcacgt gagtatacgt gattaagcac
     9721 acaaaggcag cttggagtat gtctgttatt aatttcacag gtagttctgg tccattggtg
     9781 aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg ctctagattc cgatgctgac
     9841 ttgctgggta ttatatgtgt gcccaataga aagagaacaa ttgacccggt tattgcaagg
     9901 aaaatttcaa gtcttgtaaa agcatataaa aatagttcag gcactccgaa atacttggtt
     9961 ggcgtgtttc gtaatcaacc taaggaggat gttttggctc tggtcaatga ttacggcatt
    10021 gatatcgtcc aactgcatgg agatgagtcg tggcaagaat accaagagtt cctcggtttg
    10081 ccagttatta aaagactcgt atttccaaaa gactgcaaca tactactcag tgcagcttca
    10141 cagaaacctc attcgtttat tcccttgttt gattcagaag caggtgggac aggtgaactt
    10201 ttggattgga actcgatttc tgactgggtt ggaaggcaag agagccccga aagcttacat
    10261 tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag attaaatggc
    10321 gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga ctctaacaaa
    10381 atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta tttatttaag
    10441 tattgtttgt gcacttgccg atctatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    10501 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
    10561 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
    10621 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
    10681 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
    10741 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
    10801 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
    10861 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
    10921 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
    10981 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    11041 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    11101 gtcacgacgt tgtaaaacga cggccagtga gcgcgcg
//
