LOCUS       sequence-332229-       11956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of AdmCas13d in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Li S, Li X, Xue W, Zhang L, Yang LZ, Cao SM, Lei YN, Liu CX, Guo SK,
            Shan L, Wu M, Tao X, Zhang JL, Gao X, Zhang J, Wei J, Li J, Yang L, 
            Chen LL
  TITLE     Screening for functional circular RNAs using the CRISPR-Cas13 
            system.
  JOURNAL   Nat Methods. 2021 Jan;18(1):51-59. doi: 10.1038/s41592-020-01011-4. 
            Epub 2020 Dec 7.
  PUBMED    33288960
REFERENCE   2  (bases 1 to 11956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-01011-4"; journalName: "Nat Methods"; date: 
            "2021-01"; volume: "18"; issue: "1"; pages: "51-59"
COMMENT     Sequence Label: p23-NES-AdmCas13d-msfGFP-NES-Flag
FEATURES             Location/Qualifiers
     source          1..11956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4073..4075,4076..4786)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4115..4136)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4723..4744
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4796..4822
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4847..4912
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4919..5486
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5080..5097)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5307..5326
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5506..6105
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5506..5525)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6002..6022
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6124..6712
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6177..6197)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6595..6606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6787..7020
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7139..7157)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7225..7329
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7330..7398,7399..8190)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7548..7567)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8361..8949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8850..8869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9103..9120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9360..9381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9396..9401,9402..9419,9420..9426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9434..9450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9439..9461
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9458..9474
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9458..9474
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9524..9543
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9735..10368
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10415..10540
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11037..11270
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11455..11499
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11792..11909
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccatgc tgtaccctga
     1201 gcggctgcgg cggatcctga ccgctagcaa caataagagg aagaccaagg caaaggcagc
     1261 aggcctgaag agcgtgttct ttgaccagaa gcaggccgtg ctgaccacat tcgccaaggg
     1321 caacaatagc cagatcgaga agaaggtggt gaactccgag gtgaaggatc tgagacagcc
     1381 ccctgccttc gacctggagc tgaaggagaa gaccttttac atctctggca agaacaatat
     1441 caacacaagc agggagaatc ctctggcctc tgccagcctg ccactgagca agaggcagcg
     1501 catccgggca gagcgcatca agagagccag ggaggagaac cgcccatacc acaatgtgaa
     1561 gcgggtgggc gaggacgatc tgagagccaa ggccgatctg gagaagcact acttcggcaa
     1621 ggagtattcc gacaacctga agatccagat catctataac atcctggata tcaataagat
     1681 catctctccc tacatcaacg acatcgtgta tagcatgaac aatctggcca ggaatgacga
     1741 gtacatcgat ggcaagatcg acgtgatcgg cagcctgagc tccaccacag attattctag
     1801 ctttatgtcc ccaaacaagg acctggagaa ggagaagaag ttctcttttc accgcgagaa
     1861 ttacaagaag ttcgtggagg cctccaagcc ctatatgagg tactatggca aggtgtttat
     1921 ccgcgatgtg aagaagtcta agctgagcac cggcaagggc gagaagatcg aagtgatgta
     1981 ccggtctgac gaggagatct tcacaatctt tcagatcctg agctatgtga gacagtccat
     2041 catgcacaac gatatcggca ataagtcctc tatcctggcc atcgagaagt accctgcccg
     2101 gttcgtgggc tttctgagcg acctgctgaa gaccaagaca aacgatgtga atagaatgtt
     2161 catcgacaac aattcccaga ccaacttttg ggtgctgttc tctatctttg gcctgcagga
     2221 tcacacaagc ggcgccgaca agatctgccg gaacttctac gattttgtga tcaaggccga
     2281 ctctaagaat ctgggcttca gcctgaagaa gatcagagag ctgatgctgg atctgccaaa
     2341 cgccaatatg ctgcgggatc accagtttga caccgtgaga tccaagttct acacactgct
     2401 ggacttcatc atctaccagc actatctgga ggagaagtct aggatcgaca atatggtgga
     2461 gaagctgcgc atgaccctga aggaggagga gaaggaggtg ctgtatgccg ccgaggccaa
     2521 gatcgtgtgg aacgccatcg gcgccaaagt gatcaacaag ctggtgccca tgatgaatgg
     2581 cgatgccctg aaggagatca agcggaagaa tcgcgaccgg aagctgcctc agtccgtgat
     2641 cgccacagtg caggtgaact ccgatgccaa cgtgttcagc ggcctgatct acttcctgac
     2701 cctgtttctg gacggcaagg agatcaacga gatggtgagc aatctgatca caaagtttga
     2761 gaacatcgat tccctgctgc acgtggacag ggagatctat aagtctgacg agaaggacct
     2821 ggatctggag atcgagaagc tggccctgtt ctttaagggc gtggtgcgcc ccaatgccaa
     2881 gaccgataca ggcgccggcg agatctccaa gtctttcagc atctttcaga gcgccgagcg
     2941 gatcatcgag gagctgaagt tcatcaagaa cgtgaccaga atggataatg agatcttccc
     3001 ttccgagggc gtgtttctgg acgcagcaaa cgtgctgggc gtgaggggcg acgatttcga
     3061 tttttccaac gagtttgtgg gcgacgatct gcactctgac gccaataaga agatcatcaa
     3121 caagatcaat ggcacaaagg aggaccgcaa cctgcggaat ttcatcatca acaatgtggt
     3181 gaagagccgg agatttcagt acatcgcccg ccacatgaac acccactatg tgaagcagct
     3241 ggccaacaat gagacactga acaggttcgt gctgaataag atgggcgacg ccaagatcat
     3301 caatcgctac tatgagtcca tctctggcaa cacccctaat atcgaggtgc ggtctcagat
     3361 cgattacctg gtgaagagac tgaggagctt ctcctttgag gacctgaacg atgtgaagca
     3421 gaaggtgaga ccaggcacca atgagagcat cgagaaggag aagaagaagg ccctggtggg
     3481 cctgtgcctg acaatccagt acctggtgta taagaacctg gtgaacatca atgcccggta
     3541 caccacagcc ttctattgtc tggagagaga ctctaagctg aagggcttcg gcgtggacgt
     3601 gtggcgggac tttgagagct acaccgccct gacaaaccac ttcatcaagg agggctatct
     3661 gcccgtgaga aaggccgaga tcctgagggc caatctgaag cacctggact gtgaggatgg
     3721 ctttaagtac tataggaacc aggtgaccca cctgaatgcc atccgcgtgg cctacaagta
     3781 tatcaacgag atcaagtccg tgcactctta cttcgccctg taccactata tcatgcagag
     3841 acacctgtat gactccctgc aggccaaggc caaggatagc tccggcttcg tgatcgacgc
     3901 cctgaagaag agctttgagc acaagatcta ctccaaggat ctgctgcacg tgctgcacag
     3961 ccctttcggc tacaacaccg ccaggtataa gaatctgtcc atcgaggccc tgtttgataa
     4021 gaatgagtcc cgcccagagg tgaaccccct gtctacaaat gacagtctag aggtgagcaa
     4081 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4141 cggccacaag ttcagcgtgc gcggcgaggg cgagggcgat gccaccaacg gcaagctgac
     4201 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4261 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc gccacgactt
     4321 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcagct tcaaggacga
     4381 cggcacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4441 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4501 caacttcaac agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
     4561 caacttcaag atccgccaca acgtggagga cggcagcgtg cagctcgccg accactacca
     4621 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     4681 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     4741 cgtgaccgcc gccgggatca ctcacggcat ggacgagctg tacaagtacc ttcaactgcc
     4801 tccacttgaa agactgacac tgctagagca acgcgtgcag ctaagcgact acaaagacca
     4861 tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca agtaaggatc
     4921 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     4981 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5041 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5101 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5161 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5221 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5281 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5341 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5401 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5461 ttttcctttg aaaaacacga tgataaggat ccaccggagc ttaccatgac cgagtacaag
     5521 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5581 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5641 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5701 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5761 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5821 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5881 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5941 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6001 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6061 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc atcgatagat
     6121 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6181 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6241 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6301 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6361 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6421 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6481 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6541 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6601 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6661 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc
     6721 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6781 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6841 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6901 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6961 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7021 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7081 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc
     7141 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     7201 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7261 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7321 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7381 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7441 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7501 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7561 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7621 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7681 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7741 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7801 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7861 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7921 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7981 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8041 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8101 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8161 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8221 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8281 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8341 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8401 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8461 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8521 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8581 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8641 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8701 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8761 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8821 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8881 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8941 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9001 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9061 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9121 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9181 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     9241 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     9301 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9361 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9421 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
     9481 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9541 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     9601 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     9661 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     9721 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     9781 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     9841 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     9901 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     9961 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
    10021 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
    10081 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
    10141 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
    10201 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10261 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10321 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
    10381 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
    10441 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
    10501 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
    10561 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
    10621 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
    10681 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
    10741 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
    10801 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
    10861 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
    10921 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    10981 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    11041 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    11101 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    11161 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    11221 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    11281 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    11341 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    11401 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    11461 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    11521 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    11581 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    11641 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    11701 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
    11761 cgaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    11821 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    11881 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
    11941 tttggactag tcgtga
//
