LOCUS       sequence-33223-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 T179V
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    102..236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(139..158)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     193..212
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accgggccca atgcattggc gccgcggcgc gccgcgaatt aaaaaacctc ccacacctcc
       61 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      121 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctacg
      241 taatacgact cactatagtt ctagaggctc gagaggcctt gcggccgcgg ccagattggc
      301 ctgtcgacga attcctgcag cccgatgtct ctaagacaca ctggaacagc ggatgcttgg
      361 ggagcccatc tgggtgagga ccttgtcaag ccactgcaaa ggcccattca ggtgcagctc
      421 aatccagcag ggggtactgg tcacagtctg tctcctgtac tccgctcccc agcctttgac
      481 gaagctcatg cggatggtgc acattcgggt caactggtag acagcctcaa agccctggtt
      541 gaccgactgg gccaggaggg cagcgaactc ctggttgttg aagatcttca ggttgcatcc
      601 tggtgggatc ttgcagacgg tggccgggtg ccagccatag cgctggttac agtt
//
