LOCUS       sequence-332236-         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the mammalian expression of mLOV2GIVe lit-mimicking mutation 
            I539E and the GEF-deficient mutation F1685A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Garcia-Marcos M, Parag-Sharma K, Marivin A, Maziarz M, Luebbers A, 
            Nguyen LT
  TITLE     Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a 
            rationally engineered modular protein.
  JOURNAL   Elife. 2020 Sep 16;9. pii: 60155. doi: 10.7554/eLife.60155.
  PUBMED    32936073
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-09-16"; volume: "9. pii: 60155. doi"; pages: " 
            10.7554/eLife.60155"
COMMENT     Sequence Label: pLIC-lyn11-myc-LOV2GIVe (L) (FA)
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..84
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggatgta taaaatcaaa agggaaagac agcggtaccg agctcggatc catggaacaa
       61 aaacttattt ctgaagaaga tctgggaatt ctgtacttcc aatccaatgc gttggctact
      121 acacttgaac gtattgagaa gaactttgtc attactgacc caaggttgcc agataatccc
      181 attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga agaaattttg
      241 ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt gagaaaaatt
      301 agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta tacaaagagt
      361 ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa gggagatgtc
      421 cagtacttta ttggggttca gaaggatgga actgagcatg tccgagatgc tgccgagaga
      481 gagggagtca tggagattaa gaaaactgca gaaaatgagg aagttgttac tctacaacag
      541 gctttggaag aaagcaataa gcttacctca gtacagataa agtcctcaag ttaa
//
