LOCUS       sequence-33224-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 EPSM V179T
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     51..68
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             122..145
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
       61 cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattcg aattcaccat
      121 ggactacaag gacgacgatg acaagtcgtc catcctgcct ttcactcccc cgatcgtgaa
      181 gcgcctgctg ggctggaaga agggcgagca gaacgggcag gaggagaaat ggtgcgagaa
      241 ggcggtcaag agcctggtca agaaactcaa gaagacgggg cagctggacg agctggagaa
      301 ggccatcacc acgcagaacg tcaacaccaa gtgcatcacc atccccaggt ccctggatgg
      361 ccggttgcag gtgtcccatc ggaaggggct ccctcatgtc atctactgcc gcctgtggcg
      421 atggccagac ctgcacagcc accacgagct gcgggccatg gagctgtgtg agttcgcctt
      481 caatatgaag aaggacgagg tctgcgtgaa tccctaccac taccagagag tagagacacc
      541 agttctacct cctgtgttgg tgccacgcca cacagagatc ccggccgagt tccccccact
      601 ggacgactac agccattcca tccccgaaaa cactaacttc cccgcaggca tcgagcccca
      661 gagcaatatt ccagagaccc caccccctgg ctacctgagt gaagatggag aaaccagtga
      721 ccaccagatg aaccacagca tggacgcagg tgctccaaac ctagccccga atccgatggc
      781 cccagcacat aataacttgg acctgcagcc agttacctac tgcgagccgg ccttctggtg
      841 ctccatctcc tactacgagc tgaaccagcg cgtc
//
