LOCUS       sequence-33225-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 EPSM V179T
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..51
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        33..51
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    168..302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(205..224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     259..278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
       61 ctgggtaccg ggcccaatgc attggcgccg cggcgcgccg cgaattaaaa aacctcccac
      121 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
      181 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
      241 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
      301 tctacgtaat acgactcact atagttctag actaagacac actggaacag cggatgcttg
      361 gggagcccat ctgggtgagg accttgtcaa gccactgcaa aggcccattc aggtgcagct
      421 caatccagca gggggtactg gtcacagtct gtctcctgta ctccgctccc cagcctttga
      481 cgaagctcat gcggatggtg cacattcggg tcaactggta gacagcctca aagccctggt
      541 tgaccgactg ggccaggagg gcagcgaact cctggttgtt gaagatcttc aggttgcatc
      601 ctggtgggat cttgcagacg gtggccgggt gccagccata gcgctggtta cagttgggag
      661 actggacaaa aatagcgctg tcactgaggc actctgcgaa gacctcccct ccgatgtagt
      721 agagccgcac gcctcttccg atgtgtctcc gtgtcagctc cactgctgca ttcctgttga
      781 cattggagag cagccctagg cagaagcgct ccgaattgga ggggtcggtg aagccatcca
      841 cagtcatgga tggctgcgag gcgtggaatg tctccccgac gcgctggttc agctcgtagt
      901 aggagatgga gcaccagaaa
//
