LOCUS       sequence-33227-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI HA NEDD4 DD
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          94..226
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     209..228
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        271..289
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             310..336
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtttatcaca gttaaattgc taacgcagtc agtgcttctg acacaacagt ctcgaactta
       61 agctgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac aagacaggtt
      121 taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
      181 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt ccactcccag
      241 ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc tagcctcgag
      301 accatggatt atccgtatga tgttcctgat tatgcagaat tcacgcgtgg taccgcaact
      361 tgcgcggtgg aggtgttcgg gctcctggag gacgaggaaa attcacgaat tgtgagagta
      421 agagttatag ccggaatagg ccttgccaag aaggatatat tgggagctag tgatccttac
      481 gtgagagtga cgttatatga cccaatgaat ggagttctta caagtgtgca aacaaaaacc
      541 attaaaaaga gtttgaatcc aaagtggaat gaagaaatat tattcagagt tcatcctcag
      601 cagcaccggc ttctttttga agtgtttgac gaaaaccgat tgacaagaga tgatttccta
      661 ggtcaagtgg atgttccact ttatccatta ccgacaga
//
