LOCUS       sequence-33230-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI HA NEDD4L DD
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          99..231
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     214..233
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        276..294
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             315..341
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cggtagttta tcacagttaa attgctaacg cagtcagtgc ttctgacaca acagtctcga
       61 acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac
      121 aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct
      181 gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact
      241 cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc
      301 tcgagaccat ggattatccg tatgatgttc ctgattatgc agaattcacg cgtggtaccc
      361 cgggtgggtg gctgcgcagg gccctacctg ggcgggagcg gctgcagagc cctgtccacg
      421 cggtgcctcc ccagcacggc acctcccact ccaggctgtt ggttacctgg ccgggagctg
      481 gcagagacca ggatttctcc tcgccgccgt tgctgttgtt aggggaaaca gaccatctgc
      541 atctagacct gcctctctct ccgctcccca cttcagacga gctcttcctg cctggaatct
      601 gtgatccgta tgtgaaactt tcattgtacg tagcggatga gaatagagaa cttgctttgg
      661 tccagacaaa aacaattaaa aagacactga acccaaaatg gaatgaagaa ttttatttca
      721 gggtaaaccc atctaatcac agactcctat ttgaagtatt tgacgaaaat agactgacac
      781 gagacgactt cctgggccag gtggacgtgc ccctta
//
