LOCUS       sequence-33232-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI HA NEDD4
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          80..212
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     195..214
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        257..275
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             296..322
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aattgctaac gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagaagttg
       61 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
      121 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
      181 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
      241 ttaaggctag agtacttaat acgactcact ataggctagc ctcgagacca tggattatcc
      301 gtatgatgtt cctgattatg cagaattcac gcgtggtacc gcaacttgcg cggtggaggt
      361 gttcgggctc ctggaggacg aggaaaattc acgaattgtg agagtaagag ttatagccgg
      421 aataggcctt gccaagaagg atatattggg agctagtgat ccttacgtga gagtgacgtt
      481 atatgaccca atgaatggag ttcttacaag tgtgcaaaca aaaaccatta aaaagagttt
      541 gaatccaaag tggaatgaag aaatattatt cagagttcat cctcagcagc accggcttct
      601 ttttgaagtg tttgacgaaa accgattgac aagagatgat ttcctaggtc aagtggatgt
      661 tccactttat ccattaccga cagaaaatcc aagattggag agaccatata catttaagga
      721 ttttgttctt catccaagaa gtcacaaatc aagagttaaa ggttatctga gactaaaaat
      781 gacttattta cctaaaacca gtggctcaga agatgataat gcagaacagg ctgaggaatt
      841 agagcctggc tgggttgttt tggaccaacc agatgctgct tgccatttgc agcaacaaca
      901 agaacct
//
