LOCUS       sequence-332336-        1572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9-NG-mediated genome editing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki 
            O, Ikawa M
  TITLE     Precise CAG repeat contraction in a Huntington's Disease mouse model
            is enabled by gene editing with SpCas9-NG.
  JOURNAL   Commun Biol. 2021 Jun 23;4(1):771. doi: 10.1038/s42003-021-02304-w.
  PUBMED    34163001
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-06-23"; volume: "4(1):771. doi"; pages: " 
            10.1038/s42003-021-02304-w"
COMMENT     Sequence Label: pX459-SpCas9-NG
FEATURES             Location/Qualifiers
     source          1..1572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc
       61 acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac
      121 gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc
      181 gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag
      241 cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac
      301 aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac
      361 ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag
      421 gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc
      481 attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc
      541 cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag
      601 ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc
      661 agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac
      721 ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg
      781 ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctgaagga cgactccatc
      841 gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc
      901 gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt
      961 acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat
     1021 aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca
     1081 cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa
     1141 gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt
     1201 tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc
     1261 gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac
     1321 tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct
     1381 accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg
     1441 gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc
     1501 gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg
     1561 aatatcgtga aa
//
