LOCUS       sequence-332337-        1502 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9-NG-mediated genome editing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki 
            O, Ikawa M
  TITLE     Precise CAG repeat contraction in a Huntington's Disease mouse model
            is enabled by gene editing with SpCas9-NG.
  JOURNAL   Commun Biol. 2021 Jun 23;4(1):771. doi: 10.1038/s42003-021-02304-w.
  PUBMED    34163001
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-06-23"; volume: "4(1):771. doi"; pages: " 
            10.1038/s42003-021-02304-w"
COMMENT     Sequence Label: pX459-SpCas9-NG
FEATURES             Location/Qualifiers
     source          1..1502
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1147..1194
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             1210..1263
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(1264..1283)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
       61 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
      121 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
      181 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
      241 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
      301 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
      361 aaagagtcta tccggcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
      421 gaccctaaga agtacggcgg cttcgttagc cccaccgtgg cctattctgt gctggtggtg
      481 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
      541 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
      601 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
      661 gaaaacggcc ggaagagaat gctggcctct gcccggttcc tgcagaaggg aaacgaactg
      721 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
      781 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
      841 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
      901 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
      961 gagaatatca tccacctgtt taccctgacc aatctgggag cccctcgggc cttcaagtac
     1021 tttgacacca ccatcgaccg gaaggtgtac cggagcacca aagaggtgct ggacgccacc
     1081 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     1141 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggaattc
     1201 ggcagtggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     1261 ccaatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     1321 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     1381 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     1441 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     1501 ga
//
