LOCUS       sequence-332347-       10153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For integration of Sp.pCas9-dCas9 and 
            BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Kiattisewee C, Dong C, Fontana J, Sugianto W, Peralta-Yahya P, 
            Carothers JM, Zalatan JG
  TITLE     Portable bacterial CRISPR transcriptional activation enables 
            metabolic engineering in Pseudomonas putida.
  JOURNAL   Metab Eng. 2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 
            10.1016/j.ymben.2021.04.002.
  PUBMED    33930546
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 
            10.1016/j.ymben.2021.04.002."
COMMENT     Sequence Label: pPPC001
FEATURES             Location/Qualifiers
     source          1..10153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     584..600
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1135..1668)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1857..1862,1863..1879,1880..1885))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1926..1973
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_RNA        complement(2176..2254)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             join(2550..2576,2577..2579,2580..5066,5067..5069,
                     5070..6656)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      6680..6751
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6767..6794
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        6847..6881
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     RBS             6891..6902
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     mobile_element  7670..7835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            7994..8102
                     /note="incP origin of transfer"
     rep_origin      complement(8334..8922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8414..8433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9093..9884,9885..9953))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9716..9735
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9954..10058)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10126..10144
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
     1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
     1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacctggg
     1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
     1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1981 ttttgaagct aattcgatca tgcatgttac gaaatcatcc tgtggagctt agtaggttta
     2041 gcaagatggc agcgcctaaa tgtagaatga taaaaggatt aagagattaa tttccctaaa
     2101 aatgataaaa caagcgtttt gaaagcgctt gtttttttgg tttgcagtca gagtagaata
     2161 gaagtatcaa aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct
     2221 tattttaact tgctatgctg ttttgaatgg ttccaacaag attattttat aacttttata
     2281 acaaataatc aaggagaaat tcaaagaaat ttatcagcca taaaacaata cttaatacta
     2341 tagaatgata acaaaataaa ctacttttta aaagaatttt gtgttataat ctatttatta
     2401 ttaagtattg ggtaatattt tttgaagaga tattttgaaa aagaaaaatt aaagcatatt
     2461 aaactaattt cggaggtcat taaaactatt attgaaatca tcaaactcat tatggattta
     2521 atttaaactt tttattttag gaggcaaaaa tggataagaa atactcaata ggcttagcta
     2581 tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt ccgtctaaaa
     2641 agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt ataggggctc
     2701 ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca gctcgtagaa
     2761 ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca aatgagatgg
     2821 cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg gaagaagaca
     2881 agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct tatcatgaga
     2941 aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat aaagcggatt
     3001 tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat tttttgattg
     3061 agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag ttggtacaaa
     3121 cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat gctaaagcga
     3181 ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct cagctccccg
     3241 gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt ttgaccccta
     3301 attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca aaagatactt
     3361 acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct gatttgtttt
     3421 tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga gtaaatactg
     3481 aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa catcatcaag
     3541 acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat aaagaaatct
     3601 tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct agccaagaag
     3661 aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag gaattattgg
     3721 tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac ggctctattc
     3781 cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa gacttttatc
     3841 catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga attccttatt
     3901 atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg aagtctgaag
     3961 aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca gctcaatcat
     4021 ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta ctaccaaaac
     4081 atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc aaatatgtta
     4141 ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc attgttgatt
     4201 tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat tatttcaaaa
     4261 aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt aatgcttcat
     4321 taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg gataatgaag
     4381 aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa gatagggaga
     4441 tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg atgaaacagc
     4501 ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt aatggtatta
     4561 gggataagca atctggcaaa acaatattag attttttgaa atcagatggt tttgccaatc
     4621 gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac attcaaaaag
     4681 cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta gctggtagcc
     4741 ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg gtcaaagtaa
     4801 tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat cagacaactc
     4861 aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt atcaaagaat
     4921 taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa aatgaaaagc
     4981 tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa ttagatatta
     5041 atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt aaagacgatt
     5101 caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg gataacgttc
     5161 caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta aacgccaagt
     5221 taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt ttgagtgaac
     5281 ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc actaagcatg
     5341 tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat aaacttattc
     5401 gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga aaagatttcc
     5461 aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg tatctaaatg
     5521 ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag tttgtctatg
     5581 gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa gaaataggca
     5641 aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa acagaaatta
     5701 cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg gaaactggag
     5761 aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg tccatgcccc
     5821 aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag gagtcaattt
     5881 taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat ccaaaaaaat
     5941 atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct aaggtggaaa
     6001 aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca attatggaaa
     6061 gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat aaggaagtta
     6121 aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa aacggtcgta
     6181 aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct ctgccaagca
     6241 aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt agtccagaag
     6301 ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat gagattattg
     6361 agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta gataaagttc
     6421 ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa aatattattc
     6481 atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt gatacaacaa
     6541 ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt atccatcaat
     6601 ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt gactaactcg
     6661 agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     6721 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     6781 tgggcctttc tgcgtttata cctagagcgc ccggaagaga gtcaattcag ggtggtgaat
     6841 ctgcagttta cggctagctc agccctaggt attatgctag cgaattcatt aaagaggaga
     6901 aaggtaccat ggggcccgct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa
     6961 ctggcgacgt gactgtcgcc ccaagcaact tcgctaacgg gatcgctgaa tggatcagct
     7021 ctaactcgcg ttcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga
     7081 atcgcaaata caccatcaaa gtcgaggtgc ctaaaggcgc ctggcgttcg tacttaaata
     7141 tggaactaac cattccaatt ttcgccacga attccgactg cgagcttatt gttaaggcaa
     7201 tgcaaggtct cctaaaagat ggaaacccga ttccctcagc aatcgcagca aactccggca
     7261 tctacggtgg cggaggtagc atgtcccatc agaaaattat tcaggatctt atcgcatgga
     7321 ttgacgagca tattgaccag ccgcttaaca ttgatgtagt cgcaaaaaaa tcaggctatt
     7381 caaagtggta cttgcaacga atgttccgca cggtgacgca tcagacgctt ggcgattaca
     7441 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
     7501 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttcgcgc
     7561 ggcagtttga tcgcactccc gcggattatc gccaccgcct gtaagcggcc gccacgcaaa
     7621 aaaccccgct tcggcggggt tttttcgcct cgcgaaggcc ttgcaggcca accagataag
     7681 tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac
     7741 acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca
     7801 cccctcccag ttcccaacta ttttgtccgc ccacagcggg gcatttttct tcctgttatg
     7861 tttgggcgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7921 gctcttccgc ttcctcgctc actgacccgc tgcgctcggt cgttcggctg cggcgagcgg
     7981 tatcagagct tatcggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg
     8041 aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc
     8101 ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg
     8161 tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg
     8221 gttatgcagc ggaaagtata ccttaaggaa tcccctgata acgcaggaaa gaacatgtga
     8281 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8341 aggctccgcc cccctgacga gcatcacaaa aatcgacgct cgagtcagag gtggcgaaac
     8401 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8461 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8521 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8581 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8641 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8701 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8761 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8821 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8881 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8941 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9001 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9061 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9121 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9181 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9241 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9301 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9361 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9421 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9481 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9541 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9601 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9661 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9721 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9781 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9841 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9901 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9961 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10021 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10081 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    10141 aggccctttc gtc
//
