LOCUS       sequence-332348-       11273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and 
            BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Kiattisewee C, Dong C, Fontana J, Sugianto W, Peralta-Yahya P, 
            Carothers JM, Zalatan JG
  TITLE     Portable bacterial CRISPR transcriptional activation enables 
            metabolic engineering in Pseudomonas putida.
  JOURNAL   Metab Eng. 2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 
            10.1016/j.ymben.2021.04.002.
  PUBMED    33930546
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 
            10.1016/j.ymben.2021.04.002."
COMMENT     Sequence Label: pPPC002
FEATURES             Location/Qualifiers
     source          1..11273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     584..600
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1135..1668)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1857..1862,1863..1879,1880..1885))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1926..1973
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        2183..2217
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
     RBS             2227..2238
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2245..2247,2248..2961)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2245..2958
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2275..2303)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2898..2918
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2994..3065
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3081..3108
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        complement(3290..3368)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             join(3664..3690,3691..3693,3694..6180,6181..6183,
                     6184..7770)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      7794..7865
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7881..7908
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        7961..7995
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     RBS             8005..8016
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     mobile_element  8790..8955
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            9114..9222
                     /note="incP origin of transfer"
     rep_origin      complement(9454..10042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9534..9553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10213..11004,11005..11073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10836..10855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11074..11178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11246..11264
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
     1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
     1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacctggg
     1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
     1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1981 ttttgaagct aattcgatca tgcatgctgc aggcctacgg tatccaccgg agacctatgg
     2041 cagcctccgg ccgccatagg acacctttgg ttgccaaggg tgacctatgg tgaccatggg
     2101 ccaccacggg cgacctcagg tatcctgcgg tgtcctgcgg ttaccaaagg cgtcctttgg
     2161 gttccaccgg atacctccgg acttgacagc tagctcagtc ctagggattg tgctagcgaa
     2221 ttcattaaag aggagaaagg taccatgagc aaaggagaag aacttttcac tggagttgtc
     2281 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt ccgtggagag
     2341 ggtgaaggtg atgctacaaa cggaaaactc acccttaaat ttatttgcac tactggaaaa
     2401 ctacctgttc cgtggccaac acttgtcact actctgacct atggtgttca atgcttttcc
     2461 cgttatccgg atcacatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
     2521 gtacaggaac gcactatatc tttcaaagat gacgggacct acaagacgcg tgctgaagtc
     2581 aagtttgaag gtgataccct tgttaatcgt atcgagttaa agggtattga ttttaaagaa
     2641 gatggaaaca ttcttggaca caaactcgag tacaacttta actcacacaa tgtatacatc
     2701 acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacgttgaa
     2761 gatggttccg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     2821 gtccttttac cagacaacca ttacctgtcg acacaatctg tcctttcgaa agatcccaac
     2881 gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc
     2941 atggatgagc tctacaaata aggatccaaa ctcgagtaag gatctccagg catcaaataa
     3001 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     3061 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tataaagctt
     3121 ttacgaaatc atcctgtgga gcttagtagg tttagcaaga tggcagcgcc taaatgtaga
     3181 atgataaaag gattaagaga ttaatttccc taaaaatgat aaaacaagcg ttttgaaagc
     3241 gcttgttttt ttggtttgca gtcagagtag aatagaagta tcaaaaaaag caccgactcg
     3301 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat gctgttttga
     3361 atggttccaa caagattatt ttataacttt tataacaaat aatcaaggag aaattcaaag
     3421 aaatttatca gccataaaac aatacttaat actatagaat gataacaaaa taaactactt
     3481 tttaaaagaa ttttgtgtta taatctattt attattaagt attgggtaat attttttgaa
     3541 gagatatttt gaaaaagaaa aattaaagca tattaaacta atttcggagg tcattaaaac
     3601 tattattgaa atcatcaaac tcattatgga tttaatttaa actttttatt ttaggaggca
     3661 aaaatggata agaaatactc aataggctta gctatcggca caaatagcgt cggatgggcg
     3721 gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac
     3781 cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg
     3841 gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt
     3901 tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat
     3961 cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt
     4021 ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga
     4081 aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg
     4141 catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt
     4201 gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac
     4261 cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca
     4321 agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg
     4381 aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca
     4441 gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg
     4501 gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct
     4561 attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct
     4621 tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt
     4681 cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat
     4741 gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt
     4801 ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg
     4861 cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt gggtgagctg
     4921 catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag
     4981 attgaaaaaa tcttgacttt tcgaattcct tattatgttg gtccattggc gcgtggcaat
     5041 agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa
     5101 gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat
     5161 aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg
     5221 gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt
     5281 ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta
     5341 accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa
     5401 atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa
     5461 attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt
     5521 gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat
     5581 gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg
     5641 ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata
     5701 ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat
     5761 gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt
     5821 ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag
     5881 actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc
     5941 gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag
     6001 cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat
     6061 cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga
     6121 agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat
     6181 gccattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt
     6241 tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg
     6301 aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat
     6361 ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc
     6421 caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg
     6481 aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa
     6541 tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac
     6601 aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag
     6661 aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt
     6721 aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac
     6781 tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa
     6841 cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat
     6901 tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa
     6961 gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt
     7021 attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta
     7081 gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc
     7141 gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt
     7201 gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct
     7261 aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa
     7321 ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct
     7381 agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg
     7441 gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt
     7501 gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac
     7561 aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga
     7621 gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca
     7681 aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc
     7741 attgatttga gtcagctagg aggtgactaa ctcgagtaag gatctccagg catcaaataa
     7801 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     7861 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatacctaga
     7921 gcgcccggaa gagagtcaat tcagggtggt gaatctgcag tttacggcta gctcagccct
     7981 aggtattatg ctagcgaatt cattaaagag gagaaaggta ccatggggcc cgcttctaac
     8041 tttactcagt tcgttctcgt cgacaatggc ggaactggcg acgtgactgt cgccccaagc
     8101 aacttcgcta acgggatcgc tgaatggatc agctctaact cgcgttcaca ggcttacaaa
     8161 gtaacctgta gcgttcgtca gagctctgcg cagaatcgca aatacaccat caaagtcgag
     8221 gtgcctaaag gcgcctggcg ttcgtactta aatatggaac taaccattcc aattttcgcc
     8281 acgaattccg actgcgagct tattgttaag gcaatgcaag gtctcctaaa agatggaaac
     8341 ccgattccct cagcaatcgc agcaaactcc ggcatctacg gtggcggagg tagcatgtcc
     8401 catcagaaaa ttattcagga tcttatcgca tggattgacg agcatattga ccagccgctt
     8461 aacattgatg tagtcgcaaa aaaatcaggc tattcaaagt ggtacttgca acgaatgttc
     8521 cgcacggtga cgcatcagac gcttggcgat tacattcgcc aacgccgcct gttactggcc
     8581 gccgttgagt tgcgcaccac cgagcgtccg atttttgata tcgcaatgga cctgggttat
     8641 gtctcgcagc agaccttctc ccgcgttttc gcgcggcagt ttgatcgcac tcccgcggat
     8701 tatcgccacc gcctgtaagc ggccgccacg caaaaaaccc cgcttcggcg gggttttttc
     8761 gcaagcttct cgcgaaggcc ttgcaggcca accagataag tgaaatctag ttccaaacta
     8821 ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc catctatttt
     8881 gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta
     8941 ttttgtccgc ccacagcggg gcatttttct tcctgttatg tttgggcgct gcattaatga
     9001 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9061 actgacccgc tgcgctcggt cgttcggctg cggcgagcgg tatcagagct tatcggccag
     9121 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
     9181 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata
     9241 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat
     9301 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaagtata
     9361 ccttaaggaa tcccctgata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9481 gcatcacaaa aatcgacgct cgagtcagag gtggcgaaac ccgacaggac tataaagata
     9541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     9901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    11041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    11101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    11161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    11221 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
