LOCUS       sequence-33235-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI HA NEDD4L S382A S468A
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          98..230
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     213..232
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        275..293
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             314..340
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa
       61 cttaagctgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg ttacaagaca
      121 ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg
      181 ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag gtgtccactc
      241 ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat aggctagcct
      301 cgagaccatg gattatccgt atgatgttcc tgattatgca gaattcacgc gtggtacccc
      361 gggtgggtgg ctgcgcaggg ccctacctgg gcgggagcgg ctgcagagcc ctgtccacgc
      421 ggtgcctccc cagcacggca cctcccactc caggctgttg gttacctggc cgggagctgg
      481 cagagaccag gatttctcct cgccgccgtt gctgttgtta ggggaaacag accatctgca
      541 tctagacctg cctctctctc cgctccccac ttcagacgag ctcttcctgc ctggaatctg
      601 tgatccgtat gtgaaacttt cattgtacgt agcggatgag aatagagaac ttgctttggt
      661 ccagacaaaa acaattaaaa agacactgaa cccaaaatgg aatgaagaat tttatttcag
      721 ggtaaaccca tctaatcaca gactcctatt tgaagtattt gacgaaaata gactgacacg
      781 agacgacttc ctgggccagg tggacgtgcc ccttagtcac cttccgacag aagatccaac
      841 catggagcga ccctatacat ttaaggactt tctcctcaga
//
