LOCUS       sequence-33236-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 AAGY
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             124..147
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 catgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt cgaattcacc
      121 atggactaca aggacgacga tgacaagtcg tccatcctgc ctttcactcc cccgatcgtg
      181 aagcgcctgc tgggctggaa gaagggcgag cagaacgggc aggaggagaa atggtgcgag
      241 aaggcggtca agagcctggt caagaaactc aagaagacgg ggcagctgga cgagctggag
      301 aaggccatca ccacgcagaa cgtcaacacc aagtgcatca ccatccccag gtccctggat
      361 ggccggttgc aggtgtccca tcggaagggg ctccctcatg tcatctactg ccgcctgtgg
      421 cgatggccag acctgcacag ccaccacgag ctgcgggcca tggagctgtg tgagttcgcc
      481 ttcaatatga agaaggacga ggtctgcgtg aatccctacc actaccagag agtagagaca
      541 ccagttctac ctcctgtgtt ggtgccacgc cacacagaga tcccggccga gttcccccca
      601 ctggacgact acagccattc catccccgaa aacactaact tccccgcagg catcgagccc
      661 cagagcaata ttccagagac cccagccgct ggctacctga gtgaagatgg agaaaccagt
      721 gaccaccaga tgaaccacag catggacgca ggttctccaa acctatcccc gaatccgatg
      781 tccccagcac ataataactt ggacctgcag ccagttacct actgcgagcc ggccttctgg
      841 tgctccatct cctactacga gctgaaccag cg
//
