LOCUS       sequence-33237-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 AAGY
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..37
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        19..37
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    154..288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(191..210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     245..264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg gtaccgggcc
       61 caatgcattg gcgccgcggc gcgccgcgaa ttaaaaaacc tcccacacct ccccctgaac
      121 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
      181 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      241 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcta cgtaatacga
      301 ctcactatag ttctagaggc tcgagaggcc ttgcggccgc ggccagattg gcctgtcgac
      361 gaattcctgc agcccgatgt ctctaagaca cactggaaca gcggatgctt ggggagccca
      421 tctgggtgag gaccttgtca agccactgca aaggcccatt caggtgcagc tcaatccagc
      481 agggggtact ggtcacagtc tgtctcctgt actccgctcc ccagcctttg acgaagctca
      541 tgcggatggt gcacattcgg gtcaactggt agacagcctc aaagccctgg ttgaccgact
      601 gggccaggag ggcagcgaac tcctggttgt tgaagatctt caggttgcat cctggtggga
      661 tcttgca
//
